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Pharmacology, Toxicology and Environmental Health Commons™

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Environmental Health

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2018

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Articles 1 - 30 of 49

Full-Text Articles in Pharmacology, Toxicology and Environmental Health

Resveratrol Attenuates Allergic Asthma And Associated Inflammation In The Lungs Through Regulation Of Mirna-34a That Targets Foxp3 In Mice, Esraah Alharris, Hasan Alghetaa, Ratanesh K. Seth, Saurabh Chatterjee, Narendra P. Singh, Mitzi Nagarkatti, Prakash Nagarkatti Dec 2018

Resveratrol Attenuates Allergic Asthma And Associated Inflammation In The Lungs Through Regulation Of Mirna-34a That Targets Foxp3 In Mice, Esraah Alharris, Hasan Alghetaa, Ratanesh K. Seth, Saurabh Chatterjee, Narendra P. Singh, Mitzi Nagarkatti, Prakash Nagarkatti

Faculty Publications

Asthma is a chronic inflammatory disease of airways mediated by T-helper 2 (Th2) cells involving complex signaling pathways. Although resveratrol has previously been shown to attenuate allergic asthma, the role of miRNA in this process has not been studied. We investigated the effect of resveratrol on ovalbumin-induced experimental allergic asthma in mice. To that end, BALB/c mice were immunized with ovalbumin (OVA) intraperitoneally followed by oral gavage of vehicle (OVA-veh) or resveratrol (100 mg/kg body) (OVA-res). On day 7, the experimental groups received intranasal challenge of OVA followed by 7 days of additional oral gavage of vehicle or resveratrol. At …

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Macromolecular-Clustered Facial Amphiphilic Antimicrobials, Md Anisur Rahman, Marpe Bam, Edgar Luat, Moumita Sharmin Jui, Mitra S. Ganewatta, Tinom Shokfai, Mitzi Nagarkatti, Alan W. Decho Dec 2018

Macromolecular-Clustered Facial Amphiphilic Antimicrobials, Md Anisur Rahman, Marpe Bam, Edgar Luat, Moumita Sharmin Jui, Mitra S. Ganewatta, Tinom Shokfai, Mitzi Nagarkatti, Alan W. Decho

Faculty Publications

Bacterial infections and antibiotic resistance, particularly by Gram-negative pathogens, have become a global healthcare crisis. We report the design of a class of cationic antimicrobial polymers that cluster local facial amphiphilicity from repeating units to enhance interactions with bacterial membranes without requiring a globally conformational arrangement associated with highly unfavorable entropic loss. This concept of macromolecular architectures is demonstrated with a series of multicyclic natural product-based cationic polymers. We have shown that cholic acid derivatives with three charged head groups are more potent and selective than lithocholic and deoxycholic counterparts, particularly against Gram-negative bacteria. This is ascribed to the formation …

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Watersheds In Planetary Health Research And Action, Aaron Jenkins, Anthony Capon, Joel Negin, Ben Marais, Tania Sorrell, Margot Parkes, Pierre Horwitz Dec 2018

Watersheds In Planetary Health Research And Action, Aaron Jenkins, Anthony Capon, Joel Negin, Ben Marais, Tania Sorrell, Margot Parkes, Pierre Horwitz

Research outputs 2014 to 2021

Watersheds (also known as water catchments and river basins) are recognised in contemporary science as important natural systems in which to investigate the complex socioecological foundations of health. A watershed is the spatially bound geophysical unit within which surface and shallow groundwater drain to a single collecting stream or river (see appendix). Watersheds are physical and abstract systems: they are open and hydrologically permeable, yet can be represented as functionally distinct. Collectively, watersheds comprise a complex hierarchical network, and thus exemplify the upstream and downstream nature of ecosystems. Watersheds include the social actors, relationships, and institutions located within their boundaries. …

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Aptamer-Based Assay For Detection Of Ochratoxin A, Amanda Nicole Bartley Nov 2018

Aptamer-Based Assay For Detection Of Ochratoxin A, Amanda Nicole Bartley

FIU Electronic Theses and Dissertations

Ochratoxin A (OTA) is a potent mycotoxin found in a wide range of agricultural products that has been linked to mitochondrial damage and renal disease. The standard methods for OTA analysis currently rely on the use of high-performance liquid chromatography (HPLC) coupled to fluorescence detection or mass spectrometry. Toward a highthroughput analysis of OTA, a single-stranded DNA aptamer, modified with a fluorophore, coupled to a complementary sequence, modified with a FRET-based quencher that dissociates in the presence of the target toxin, is proposed. In order to integrate “target trapping,” aptamer immobilization methods were explored to mediate interference issues. Assays were …

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A Qualitative Assessment Of Disability Friendly Water And Sanitation Facilities In Primary Schools, Rumphi, Malawi, Harlod Zaunda, Rochelle H. Holm, Ambumulire Itimu-Phiri, Mphatso Malota, Sian White Nov 2018

A Qualitative Assessment Of Disability Friendly Water And Sanitation Facilities In Primary Schools, Rumphi, Malawi, Harlod Zaunda, Rochelle H. Holm, Ambumulire Itimu-Phiri, Mphatso Malota, Sian White

Faculty Scholarship

Students with disabilities commonly face barriers when accessing water and using sanitation and hygiene facilities at school. International frameworks have prompted governments to enact local policies that enshrine these rights, guarantee equitable access to education and mandate inclusive infrastructure. This research was designed to explore whether Malawi has translated good policies into practice. Data were gathered in Rumphi district, Malawi, through structured field observations in ten schools and interviews with students with a disability (n = 23), teachers (n = 11) and government stakeholders (n = 2). No school had facilities that fully meet the needs of students with disabilities, …

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Addressing Environmental Gentrification: Improving Environmental Health For Children And Youth Without Displacement, Tania Schusler, Amy Krings Nov 2018

Addressing Environmental Gentrification: Improving Environmental Health For Children And Youth Without Displacement, Tania Schusler, Amy Krings

Social Work: School of Social Work Faculty Publications and Other Works

Historically, low income communities and communities of color have borne the brunt of environmental pollution with limited access to environmental amenities. This has arisen, in part, due to marginalized communities’ lack of access and influence in environmental decision making, as well as legacies of racial and ethnic housing segregation (Cole & Foster, 2001). Environmental justice concerns include the disproportionate placement and inequitable regulation of polluting facilities in areas populated by people who are poor and/or racial minorities (Bryant, 1995: Bullard, 1993; Mohai & Bryant, 1992; Lavelle & Coyle, 1992). The inequitable distribution of environmental pollution is especially problematic for children’s …

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Rationale, Design And Methods Of "Set The Rules": A Tailored Peer-To-Peer Health Information Intervention, Jennifer R. Warren, Brandi M. White Oct 2018

Rationale, Design And Methods Of "Set The Rules": A Tailored Peer-To-Peer Health Information Intervention, Jennifer R. Warren, Brandi M. White

Health and Clinical Sciences Faculty Publications

Ensuring equitable access to health information is one strategy to promote health equity for underserved communities, especially for low-income African Americans (AAs). Childcare centers are one viable site to deliver health information to address this disparity. This paper describes the methods used in a community-based participatory research project with a childcare facility that aimed to reduce environmental tobacco smoke (ETS) exposure among low-income AA children. Through collaboration and multiple data collection methods, partners identified communication strategies to overcome informational barriers. These initial findings indicated a peer-to-peer health information intervention, entitled “Set the Rules”, as the best strategy to increase awareness. …

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La Asociación Entre La Contaminación Del Agua Y La Salud En Los Niños Menores De Cinco Años En Villa Garrote, Partido De Tigre / The Association Between Water Pollution And Health In Children Under Five In Villa Garrote, Tigre Party, Morgan Whyte Oct 2018

La Asociación Entre La Contaminación Del Agua Y La Salud En Los Niños Menores De Cinco Años En Villa Garrote, Partido De Tigre / The Association Between Water Pollution And Health In Children Under Five In Villa Garrote, Tigre Party, Morgan Whyte

Independent Study Project (ISP) Collection

La intersección entre el medio ambiente y la salud es un tema central en el estudio de la salud pública porque el acceso a un medio ambiente seguro es esencial para los resultados positivos en la salud. Este tema es particularmente relevante al considerar a Villa Garrote, una pequeña villa situada en el río Reconquista en el municipio de Tigre. Para abordar este tema dentro de esta villa, esta investigación pretende explorar la asociación entre la contaminación del agua y la salud de los niños. Además, los objetivos específicos de este estudio incluyen: investigar los principales elementos que contaminan el …

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Autonomous Cars, Electric And Hybrid Cars, And Ridesharing: Perceptions Vs. Reality, Christopher W. Eni, Charlie J. Clarke, Danielle A. Wyatt Oct 2018

Autonomous Cars, Electric And Hybrid Cars, And Ridesharing: Perceptions Vs. Reality, Christopher W. Eni, Charlie J. Clarke, Danielle A. Wyatt

Student Publications

Autonomous Cars, Electric and Hybrid Cars, and Ridesharing are all important new technologies in today's society that can have potentially large impacts on the environment in the future. This study was conducted to determine the differences in perceptions of Gettysburg College students regarding Autonomous Cars, Electric and Hybrid Cars, and Ridesharing and the reality of these topics in the real world. This paper also compares the perceptions of Environmental Studies majors/minors to the perceptions of other majors at Gettysburg College. The primary research was conducted by analyzing questions that were a part of a survey consisting of 16 questions which …

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Perceptions And Physical Realities Of Flood Risk In Cerro Punta, Chiriquí, Panamá, Julia Stanganelli Oct 2018

Perceptions And Physical Realities Of Flood Risk In Cerro Punta, Chiriquí, Panamá, Julia Stanganelli

Independent Study Project (ISP) Collection

Flooding is an issue of increasing global importance. With climate change and land-use alteration affecting hydrological cycles globally, certain regions of the world are experiencing more frequent or severe flooding. In the Chiriquí Province of Panamá, the town of Cerro Punta has been subject to several severe flooding events in the last few decades. This study aimed to understand perceptions of flood risk amongst residents of Cerro Punta, as well as evaluate physical flood risk in the area. Residents were interviewed regarding their perceptions of flooding as an increasing issue, possible causes, vulnerable areas and populations, and preparedness for future …

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Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (Rdx) Reduction By Granular Zero-Valent Iron In Continuous Flow Reactor, Amalia Terracciano, Jie Ge, Agamemnon Koutsospyros, Xiaoguang Meng, Benjamin Smolinski, Per Arienti Oct 2018

Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (Rdx) Reduction By Granular Zero-Valent Iron In Continuous Flow Reactor, Amalia Terracciano, Jie Ge, Agamemnon Koutsospyros, Xiaoguang Meng, Benjamin Smolinski, Per Arienti

Civil Engineering Faculty Publications

Wastewater streams containing hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX) are subject to regulatory discharge regulations that require processing through industrial waste treatment. Thus, the development of easy-to-apply technologies for the treatment of RDX-laden wastewater streams is imperative. In the present study, the reduction of RDX by granular zero valent iron (GZVI) in batch and column experiments was investigated. Preliminary batch tests conducted under both oxic and anoxic conditions showed that after 3.0 hours of reaction with GZVI, RDX was mainly converted to formaldehyde (CH2O), nitrate (NO3 - ), and ammonium (NH4 + ). Column filtration tests …

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He Effects Of Climate Change On Native Icelandic Plants: A Morphometric Analysis, Maia Jones Oct 2018

He Effects Of Climate Change On Native Icelandic Plants: A Morphometric Analysis, Maia Jones

Independent Study Project (ISP) Collection

This study seeks to evaluate the morphology of three native Icelandic plant species, Alchemilla alpina, Arabidopsis petraea, and Silene uniflora, in order to determine if changing climatic conditions have had an effect on Icelandic vegetation during the twentieth century. I measured herbarium specimens collected during a time period before 1950, and during the period from the late 1960s to the present. Using traditional morphometrics methods, I searched for changes in morphological traits such as plant height, leaf length to width ratio, and leaf size, then performed two-tailed t-tests to analyze the results of the analyses. I found significant changes …

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Abundance, Density, And Influencing Indicators Of Solid Waste Accumulation And Mitigation Solutions Along Coastlines On Isla Colón In Bocas Del Toro Province, Panamá, Andy Rhodes Oct 2018

Abundance, Density, And Influencing Indicators Of Solid Waste Accumulation And Mitigation Solutions Along Coastlines On Isla Colón In Bocas Del Toro Province, Panamá, Andy Rhodes

Independent Study Project (ISP) Collection

Solid waste accumulation is a significant issue in Latin America where poor infrastructure and management systems are present and with the exponential tourism growth in this region, the abundance of solid waste is also growing. This increase affects environmental, economic, and social factors such as pollution, a decline in tourism, and an increase in negative health effects. This study on Isla Colón, in the Province of Bocas del Toro, in western Panamá sought results on the abundance of solid waste along roadsides and beaches in the most populous and touristic locations. Approximately 2,160 meters squared were studied in Playa Bluff, …

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Three Land Use Proposals For Geldinganes Framed By The City Of Reykjavik’S Municipal Plan And Climate Neutrality Goals, Emma Hokoda Oct 2018

Three Land Use Proposals For Geldinganes Framed By The City Of Reykjavik’S Municipal Plan And Climate Neutrality Goals, Emma Hokoda

Independent Study Project (ISP) Collection

With global CO2 levels rising every year, climate change is becoming a larger, looming threat. Cities around the world have an important role in reducing the negative impacts of climate change through strategic and sustainable urban planning. The purpose of this study is to conduct an introductory analysis of Geldinganes island and its potential and capacity for land development that will support and expand upon the city of Reykjavik’s 2010-2030 municipal plan and 2040 climate neutrality goals. Within this study three proposals for Geldinganes are considered, the current proposal and two new proposals. The lowest development impact proposal for Geldinganes …

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Macro Implications Of Microplastics: A Comparative Study Of Microplastic Distribution In Bahía Almirante, Bocas Del Toro, Panama, Bonnie Feldberg Oct 2018

Macro Implications Of Microplastics: A Comparative Study Of Microplastic Distribution In Bahía Almirante, Bocas Del Toro, Panama, Bonnie Feldberg

Independent Study Project (ISP) Collection

Plastic is one of the most demanded materials in the modern world as it is durable and long lasting. However, that which makes it so commercially appealing also makes it environmentally degrading. Anthropogenic waste and specifically microplastics have been identified in natural habitats globally, with particular interest placed on marine ecosystems. This research aims to add to this pool by comparing microplastic concentrations in beach, mangrove, and shallow ocean sediment in Bahía Almirante, Bocas del Toro, Panama. Sediment samples were collected from each habitat type, processed, and then analyzed to obtain the number of microplastic particles per gram of dry …

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It's About Communities: The Commitment To Promoting A Culturally Competent Environmental Health Workforce, Clint Pinion Jr., Leslie D. Mitchell, Jason W. Marion Sep 2018

It's About Communities: The Commitment To Promoting A Culturally Competent Environmental Health Workforce, Clint Pinion Jr., Leslie D. Mitchell, Jason W. Marion

EKU Faculty and Staff Scholarship

Environmental health and public health are profoundly local. The Association of Environmental Health Academic Programs (AEHAP) firmly agrees and for this reason, it is important to have local environmental health experts who know the pulse of their communities. AEHAP believes in supporting the advanced scientific education of environmental health in these communities through people from these communities. Accordingly, AEHAP has sought to promote and support accredited environmental health programs among a diverse cross-section of the U.S. higher education landscape. AEHAP’s students are diverse in many ways, including socioeconomically, racially, ethnically, and culturally. The value of this approach enhances the overall …

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Influenza Virus Infectivity Is Retained In Aerosols And Droplets Independent Of Relative Humidity, Karen A. Kormuth, Kaisen Lin, Aaron J. Prussin, Eric P. Vejerano, Andrea J. Tiwari, Steve S. Cox, Micheal M. Myerburg, Seema S. Lakdawala, Linsey C. Marr Sep 2018

Influenza Virus Infectivity Is Retained In Aerosols And Droplets Independent Of Relative Humidity, Karen A. Kormuth, Kaisen Lin, Aaron J. Prussin, Eric P. Vejerano, Andrea J. Tiwari, Steve S. Cox, Micheal M. Myerburg, Seema S. Lakdawala, Linsey C. Marr

Faculty Publications

Pandemic and seasonal influenza viruses can be transmitted through aerosols and droplets, in which viruses must remain stable and infectious across a wide range of environmental conditions. Using humidity-controlled chambers, we studied the impact of relative humidity on the stability of 2009 pandemic influenza A(H1N1) virus in suspended aerosols and stationary droplets. Contrary to the prevailing paradigm that humidity modulates the stability of respiratory viruses in aerosols, we found that viruses supplemented with material from the apical surface of differentiated primary human airway epithelial cells remained equally infectious for 1 hour at all relative humidities tested. This sustained infectivity was …

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Macrophages But Not Astrocytes Harbor Hiv Dna In The Brains Of Hiv-1-Infected Aviremic Individuals On Suppressive Antiretroviral Therapy, Allen Ko, Guobin Kang, Julian B. Hattler, Hadiza I. Galadima, Junfeng Zhang, Qingsheng Li, Woong-Ki Kim Sep 2018

Macrophages But Not Astrocytes Harbor Hiv Dna In The Brains Of Hiv-1-Infected Aviremic Individuals On Suppressive Antiretroviral Therapy, Allen Ko, Guobin Kang, Julian B. Hattler, Hadiza I. Galadima, Junfeng Zhang, Qingsheng Li, Woong-Ki Kim

Community & Environmental Health Faculty Publications

The question of whether the human brain is an anatomical site of persistent HIV-1 infection during suppressive antiretroviral therapy (ART) is critical, but remains unanswered. The presence of virus in the brains of HIV patients whose viral load is effectively suppressed would demonstrate not only the potential for CNS to act as an anatomical HIV reservoir, but also the urgent need to understand the factors contributing to persistent HIV behind the blood-brain barrier. Here, we investigated for the first time the presence of cells harboring HIV DNA and RNA in the brains from subjects with undetectable plasma viral load and …

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Cross Photoreaction Of Glyoxylic And Pyruvic Acids In Model Aqueous Aerosol, Sha-Sha Xia, Alexis J. Eugene, Marcelo I. Guzman Jul 2018

Cross Photoreaction Of Glyoxylic And Pyruvic Acids In Model Aqueous Aerosol, Sha-Sha Xia, Alexis J. Eugene, Marcelo I. Guzman

Chemistry Faculty Publications

Aerosols of variable composition, size, and shape are associated with public health concerns as well as with light-particle interactions that play a role in the energy balance of the atmosphere. Photochemical reactions of 2-oxocarboxylic acids in the aqueous phase are now known to contribute to the total secondary organic aerosol (SOA) budget. This work explores the cross reaction of glyoxylic acid (GA) and pyruvic acid (PA) in water, the two most abundant 2-oxocarboxylic acids in the atmosphere, under solar irradiation and dark thermal aging steps. During irradiation, PA and GA are excited and initiate proton-coupled electron transfer or hydrogen abstraction …

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Aerosol Microdroplets Exhibit A Stable Ph Gradient, Haoran Wei, Eric P. Vejerano, Weinan Leng, Qishen Haung, Marjorie R. Willner, Linsey C. Marr, Peter J. Vikesland Jul 2018

Aerosol Microdroplets Exhibit A Stable Ph Gradient, Haoran Wei, Eric P. Vejerano, Weinan Leng, Qishen Haung, Marjorie R. Willner, Linsey C. Marr, Peter J. Vikesland

Faculty Publications

Suspended aqueous aerosol droplets (<50 μm) are microreactors for many important atmospheric reactions. In droplets and other aquatic environments, pH is arguably the key parameter dictating chemical and biological processes. The nature of the droplet air/ water interface has the potential to significantly alter droplet pH relative to bulk water. Historically, it has been challenging to measure the pH of individual droplets because of their inaccessibility to conventional pH probes. In this study, we scanned droplets containing 4-mercaptobenzoic acid–functionalized gold nanoparticle pH nanoprobes by 2D and 3D laser confocal Raman microscopy. Using surface-enhanced Raman scattering, we acquired the pH distribution inside approximately 20-μm-diameter phosphate-buffered aerosol droplets and found that the pH in the core of a droplet is higher than that of bulk solution by up to 3.6 pH units. This finding suggests the accumulation of protons at the air/water interface and is consistent with recent thermodynamic model results. The existence of this pH shift was corroborated by the observation that a catalytic reaction that occurs only under basic conditions (i.e., dimerization of 4-aminothiophenol to produce dimercaptoazobenzene) occurs within the high pH core of a droplet, but not in bulk solution. Our nanoparticle probe enables pH quantification through the cross-section of an aerosol droplet, revealing a spatial gradient that has implications for acid-base–catalyzed atmospheric chemistry.

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High Circulatory Leptin Mediated Nox-2-Peroxynitrite-Mir21 Axis Activate Mesangial Cells And Promotes Renal Inflammatory Pathology In Nonalcoholic Fatty Liver Disease, Firas Alhasson, Ratanesh K. Seth, Sutapa Sarkar, Diana A. Kimono, Muayad S. Albadrani, Diptadip Dattaroy, Varun Chandrashekaran, Geoffry Scott, Samir Raychoudhury, Mitzi Nagarkatti, Prakash Nagarkatti, Anna Mae Diehl, Saurabh Chatterjee Jul 2018

High Circulatory Leptin Mediated Nox-2-Peroxynitrite-Mir21 Axis Activate Mesangial Cells And Promotes Renal Inflammatory Pathology In Nonalcoholic Fatty Liver Disease, Firas Alhasson, Ratanesh K. Seth, Sutapa Sarkar, Diana A. Kimono, Muayad S. Albadrani, Diptadip Dattaroy, Varun Chandrashekaran, Geoffry Scott, Samir Raychoudhury, Mitzi Nagarkatti, Prakash Nagarkatti, Anna Mae Diehl, Saurabh Chatterjee

Faculty Publications

High circulatory insulin and leptin followed by underlying inflammation are often ascribed to the ectopic manifestations in non-alcoholic fatty liver disease (NAFLD) but the exact molecular pathways remain unclear. We have shown previously that CYP2E1-mediated oxidative stress and circulating leptin in NAFLD is associated with renal disease severity. Extending the studies, we hypothesized that high circulatory leptin in NAFLD causes renal mesangial cell activation and tubular inflammation via a NOX2 dependent pathway that upregulates proinflammatory miR21. High-fat diet (60% kcal) was used to induce fatty liver phenotype with parallel insulin and leptin resistance. The kidneys were probed for mesangial cell …

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Variation In Airborne Pollen Concentrations Among Five Monitoring Locations In A Desert Urban Environment, Tanviben Y. Patel, Mark P. Buttner, David Rivas, Chad L. Cross, Dennis A. Bazylinski, Joram S. Seggev Jun 2018

Variation In Airborne Pollen Concentrations Among Five Monitoring Locations In A Desert Urban Environment, Tanviben Y. Patel, Mark P. Buttner, David Rivas, Chad L. Cross, Dennis A. Bazylinski, Joram S. Seggev

Environmental & Occupational Health Faculty Publications

The urbanization of the Las Vegas Valley has transformed this part of the Mohave Desert into a green oasis by introducing many non-native plant species, some of which are allergenic. Typically, one monitoring station is established per city to obtain pollen counts for an entire metropolitan area. However, variations in pollen concentrations could occur among different microenvironments. The objective of this study is to measure and compare pollen concentrations in five locations in Las Vegas to determine if there are significant differences between microenvironments within the city. Air samples were collected from five sites across the Las Vegas Valley over …

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Developing Ocean Color Algorithm Using Moderate Resolution Imaging Spectroradiometer (Modis) Sensor For Shallow Coastal Water Bodies, Mohd Manzar Abbas Jun 2018

Developing Ocean Color Algorithm Using Moderate Resolution Imaging Spectroradiometer (Modis) Sensor For Shallow Coastal Water Bodies, Mohd Manzar Abbas

FIU Electronic Theses and Dissertations

This study analyses the spatial and temporal variability of chlorophyll-a in Chesapeake Bay; assesses the performance of Ocean Color 3M (OC3M) algorithm; and develops a novel algorithm to estimate chlorophyll-a for coastal shallow water. The OC3M algorithm yields an accurate estimate of chlorophyll-a concentration for deep ocean water (RMSE=0.016), but it failed to perform well in the coastal water system (RMSE=23.17) of Chesapeake Bay. A novel algorithm was developed which utilizes green and red bands of the Moderate Resolution Imaging Spectroradiometer (MODIS) sensor. The novel algorithm derived the chlorophyll-a concentration more accurately in Chesapeake Bay (RMSE=4.20) than the OC3M algorithm. …

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Cloning And Characterization Of A Pyrethroid Pesticide Decomposing Esterase Gene, Est3385, From Rhodopseudomonas Palustris Psb-S, Xiangwen Luo, Deyong Zhang, Xuguo Zhou, Jiao Du, Songbai Zhang, Yong Liu May 2018

Cloning And Characterization Of A Pyrethroid Pesticide Decomposing Esterase Gene, Est3385, From Rhodopseudomonas Palustris Psb-S, Xiangwen Luo, Deyong Zhang, Xuguo Zhou, Jiao Du, Songbai Zhang, Yong Liu

Entomology Faculty Publications

Full length open reading frame of pyrethroid detoxification gene, Est3385, contains 963 nucleotides. This gene was identified and cloned based on the genome sequence of Rhodopseudomonas palustris PSB-S available at the GneBank. The predicted amino acid sequence of Est3385 shared moderate identities (30–46%) with the known homologous esterases. Phylogenetic analysis revealed that Est3385 was a member in the esterase family I. Recombinant Est3385 was heterologous expressed in E. coli, purified and characterized for its substrate specificity, kinetics and stability under various conditions. The optimal temperature and pH for Est3385 were 35 °C and 6.0, respectively. This enzyme could …

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Development And Application Of Aquatic Toxicology Studies For The Assessment Of Impacts Due To Chemical Stressors Using Non-Standard Indigenous Organisms, Abraham Jeffrey Smith Apr 2018

Development And Application Of Aquatic Toxicology Studies For The Assessment Of Impacts Due To Chemical Stressors Using Non-Standard Indigenous Organisms, Abraham Jeffrey Smith

FIU Electronic Theses and Dissertations

Research in the multidisciplinary science of ecotoxicology is crucial to assess injuries to ecosystem resources from chemical spills or other stressors used to support environmental decision-making. Established guidelines recommend the use of non-standard native species in toxicity investigations. This work focused on the use of native species for aquatic toxicity assessment to make more relevant conclusions on the potential for adverse biological effects to occur as a result to single chemical exposures or exposures to a complex mixture like oil. We apply these studies to investigate petroleum product impacts from the Deepwater Horizon incident and concerns for metal toxicity in …

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Nitrogen Contamination Of Groundwater In Proximity To A Closed Landfill And Active Agricultural Fields, Brooke Daniel Apr 2018

Nitrogen Contamination Of Groundwater In Proximity To A Closed Landfill And Active Agricultural Fields, Brooke Daniel

Student Writing

Carcinogenic compounds in drinking water are becoming a significant health problem. Increased use of artificial fertilizers, disposal of wastes, and land use are the main factors responsible for increased nitrate levels in groundwater over the last 30 years. This study examined the correlation between nitrate levels and proximity to a closed, inactive garbage dump located in Middlesex, Virginia. This study also examined the correlation between nitrate levels and proximity to agricultural fields. Twenty-five sampling sites were identified on the GIS tax maps of Middlesex County, Virginia. Fifteen sites were identified within a two kilometer radius of the VPPSA landfill, also …

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Barriers To The Elimination Of Plastic In Single Use Beverage Containers In Byron Bay, Nsw, Katherine Senechal Apr 2018

Barriers To The Elimination Of Plastic In Single Use Beverage Containers In Byron Bay, Nsw, Katherine Senechal

Independent Study Project (ISP) Collection

Plastic debris is a global threat to the natural environment. The accumulation of plastic debris has resulted in the destruction of marine habitat as well as a threat to marine wildlife. Single use plastics, including plastics associated with single use food and beverage containers are contributing to a considerable amount of debris and other litter in the world’s oceans. As awareness of this issue spreads, both large corporations and small businesses are beginning to transition to plastic alternatives for their single use beverage containers. In Byron Bay, Australia, an area of increasing environmental awareness, businesses are beginning to reduce single …

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How Does Foreign Direct Investment (Fdi) Affect Environmental Pollution, Ming Wie Khoo, Michael Davidsson Apr 2018

How Does Foreign Direct Investment (Fdi) Affect Environmental Pollution, Ming Wie Khoo, Michael Davidsson

Paper Presentations

The Research paper I am presenting is to find out the effect of FDI towards environmental pollution. Headlines of New York Times on June 15, 2011 found high level of lead concentration in 233 adults and 99 children which could lead to brain, kidney, liver, nerves, stomach damage and could lead to death. The purpose of my study is to validate three hypotheses which is the pollution haven hypothesis, endowments factor hypothesis, and chain effect hypothesis made by Soilita, 2005.The dependent variable would be environmental pollution while the independent variable of the study would be bird, fish, and plant species …

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Water Capacity Of Paramo Soils, Rafael Robert Apr 2018

Water Capacity Of Paramo Soils, Rafael Robert

Independent Study Project (ISP) Collection

The pȧramo ecosystems in the high Ecuadorian Andes are grasslands characterized by challenging abiotic factors such as low temperatures and high solar radiation. Another key feature of pȧramos is the humidity and water availability in the ecosystem. Pȧramos are very important to the hydrology of the inter-Andean valley because they are water reserves that distribute fresh water to lower elevations. These hydrologic properties are made possible by the porous volcanic ash soils formed by the volcanic arc along the mountain range. This study is focused in the region of Chimborazo approximately 20 km from Riobamba the capitol city of the …

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An Inordinate Fondness For Beetles: A Study Of Insect Species Diversity And Abundance In Mazumbai Forest Reserve Versus Nearby Agricultural Areas, Emma Weisner Apr 2018

An Inordinate Fondness For Beetles: A Study Of Insect Species Diversity And Abundance In Mazumbai Forest Reserve Versus Nearby Agricultural Areas, Emma Weisner

Independent Study Project (ISP) Collection

This study investigates how human disturbance of ecosystems alters insect diversity and abundance, specifically exploring how insect communities inside Mazumbai Forest Reserve in Tanzania differ from insect communities in agricultural areas near the reserve. Following methods of previous studies on the effect of disturbance on insect populations (Bellamy et al. 2018; McLaughlin & Mineau 1995; Perry et al. 2016), this research utilizes pitfall traps and yellow bowl traps in multiple locations throughout the two study areas to catch insects, which are then identified to their specific order. The collected data support the hypothesis that insect order diversity and abundance vary …

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