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Full-Text Articles in Pharmacology, Toxicology and Environmental Health
Diversity Of Bacteriophage In Burkholderia Species, Abigail Price
Diversity Of Bacteriophage In Burkholderia Species, Abigail Price
Honors Projects
Bacteriophages are viruses that infect and replicate within bacteria and offer the potential of a therapeutic alternative to chronic infections that do not respond to antibiotic-based therapies. B. vietnamiensis is one of a number of Burkholderia species involved with chronic drug resistant infections in the lungs of individuals with compromised respiratory systems, as found in those with chronic obstructive pulmonary disease and, most especially, are of particular significance in patients with cystic fibrosis. The diversity of the Burkholderia species is explored by using online databases and looking at bacteriophage or phage-encoding viruses found in B. vietnamiensis. The open reading frames …
Determining The Effects Of Imidacloprid On Non-Target Soil Organisms In Hemlock Stands, Braley Burke
Determining The Effects Of Imidacloprid On Non-Target Soil Organisms In Hemlock Stands, Braley Burke
Graduate Theses, Dissertations, and Problem Reports
The hemlock woolly adelgid (HWA), Adelges tsugae Annand (Hemiptera: Adelgidae), is an invasive insect that is causing mortality of eastern hemlock trees, Tsuga canadensis, and Carolina hemlock trees, Tsuga caroliniana, across the eastern United States. To protect these ecologically important tree species, a neonicotinoid insecticide, imidacloprid, is commonly used. Imidacloprid is an effective treatment and can remain effective against HWA for four to six years but long-term (≥ one year after application) non-target effects of imidacloprid on forest ecosystems are not well-studied. This study examined terrestrial non-target effects of imidacloprid in hemlock stands with different treatment histories to …
Development, Validation, And Application Of A Novel Method To Separate And Quantify Silver Intracellular Uptake In Pseudomonas Aeruginosa, Mohammed A. Othman
Development, Validation, And Application Of A Novel Method To Separate And Quantify Silver Intracellular Uptake In Pseudomonas Aeruginosa, Mohammed A. Othman
Theses and Dissertations
Silver nanoparticles (AgNPs) have attracted tremendous attention as a potential broadspectrum antimicrobial agent to overcome multidrug resistant (MDR) infections. However, a comprehensive understanding to AgNPs bactericidal mechanism of action and the relative role of particulate versus ionic Ag in AgNPs antibacterial activity is lacking but essential for their optimization for potential medical applications. Therefore, a novel method to separate and quantify Ag internalization in P. aeruginosa was developed and validated through multimethod approach. The methods used were optical density at 600 nm (OD600), LIVE/DEAD staining, transmission electron microscopy (TEM), and sorbed Ag liberation assays. After optimizing the method, it was …
Estimation Of Aerosolized Antibiotic Resistant Bacteria Concentrations From Wastewater Treatment Facilities Using Spatially-Based Dispersion Modeling, Renee Nichole Dickman
Estimation Of Aerosolized Antibiotic Resistant Bacteria Concentrations From Wastewater Treatment Facilities Using Spatially-Based Dispersion Modeling, Renee Nichole Dickman
Theses and Dissertations
Pathogenic bacteria can develop resistance to antibiotics to which they were previously sensitive, resulting in increased morbidity and mortality worldwide [1]. The increase in drug resistance in bacteria is an emerging public health concern. The activated sludge tanks of wastewater treatment plants are known sources of antibiotic resistant bacteria. These resistant bacteria can become aerosolized and disperse downwind. Using previously sampled aerosol concentrations and meteorological data, a Gaussian dispersion model was developed to estimate the concentration downwind from a selected wastewater treatment facility. The emission rate was calculated to be 7,941 CFU/m²/sec ± 2,149 CFU/m²/sec. This value was used in …
Heavy Metal Toxicity In Bioremediation: Microbial Cultures And Microscopy, Jason B. Goodbody
Heavy Metal Toxicity In Bioremediation: Microbial Cultures And Microscopy, Jason B. Goodbody
Theses and Dissertations
This research employed a variety of microscopy and spread plating techniques to observe the effects of heavy metal treatments on a toluene-selected bacterial population. Microbial colonies were cultured on spread plates and the resulting numbers were compared to respiration data. The mechanisms of reproduction were demonstrated to be more sensitive to metal treatments than were the mechanisms of respiration. Phase contrast, Gram stain, fluorescent microscopy, were used to compare and document a wide variety of bacteria resulting from different metal treatments as well as from environmental changes within the source bioreactor. The removal of sensitive bacteria and the selection of …