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Examination Of Methylation Sites For Forensic Age Determination From Semen, Christian Renwick, Sarah J. Seashols-Williams

Undergraduate Research Posters

Methylation Sensitive High-Resolution Melt (MS-HRM) is based on quantitating the melt curve from an experimental sample against a standard of known methylation levels. Whereas most applications of age prediction using methylation markers are based upon pyrosequencing or SNaPshot technologies, these analysis methods are both cost and instrumentation prohibitive. This study sought to use to the varied methylation status of the ELOVL2 and FHL2 alleles, both having known correlation with age (Hamano et. al.), in a labor and time efficient manner to develop an age prediction model. A non-linear regression and standard curve was compiled from the methylation status in a …