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Development Of A Bacteriophage-Based Portable Biosensor For The Detection Of Shiga-Toxin Producing Escherichia Coli (Stec) Strains In Food And Environmental Matrices, Irwin A. Quintela
Development Of A Bacteriophage-Based Portable Biosensor For The Detection Of Shiga-Toxin Producing Escherichia Coli (Stec) Strains In Food And Environmental Matrices, Irwin A. Quintela
Electronic Theses and Dissertations
A fast and reliable on-site foodborne pathogens screening can reduce the incidence of foodborne illnesses, hospitalizations and economic loss. It can also circumvent conventional laboratory-based tests with minimal sample treatments and shorter turnaround time. Rapid detection of biological hazards has been largely dependent on immunological agents (ie antibodies). Antibodies are expensive to manufacture and experience cross-reactivity, instability with shorter shelf life. Our aim was to improve the screening process of Shiga-toxin producing Escherichia coli (STEC) strains in food and environmental matrices by developing a novel, inexpensive handheld bacteriophage-based amperometric biosensor that can directly detect live STEC cells.This biosensor development began …