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University of Windsor

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Distribution And Relative Abundance Of Reintroduced Atlantic Salmon, Salmo Salar, In Lake Ontario Tributaries Using Environmental Dna, Nabeelah Lulat Jan 2023

Distribution And Relative Abundance Of Reintroduced Atlantic Salmon, Salmo Salar, In Lake Ontario Tributaries Using Environmental Dna, Nabeelah Lulat

Electronic Theses and Dissertations

Plans to reintroduce Atlantic salmon in Lake Ontario tributaries consists of stocking hatchery-reared fish yearly which will help to achieve a self-sustaining population. The issue with reintroduction remains in understanding the distribution of fishes after stocking. Environmental DNA (eDNA) provides a sensitive approach for monitoring that can offer inferences into fish distribution. I determined the distribution of stocked Atlantic salmon downstream from stocking sites using qRT-PCR and metabarcoding. I found that Atlantic salmon eDNA detection was more sensitive using qRT-PCR (51%) versus metabarcoding HTS (18.3%). However, metabarcoding provided data on fish community assemblages, which can help to monitor ecological interactions. …