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Quantitative Pcr Assays For Detection Of Five Arctic Fish Species: Lota Lota, Cottus Cognatus, Salvelinus Alpinus, Salvelinus Malma, And Thymallus Arcticus From Environmental Dna, Torrey W. Rodgers, John R. Olson, Stephen L. Klobucar, Karen E. Mock
Quantitative Pcr Assays For Detection Of Five Arctic Fish Species: Lota Lota, Cottus Cognatus, Salvelinus Alpinus, Salvelinus Malma, And Thymallus Arcticus From Environmental Dna, Torrey W. Rodgers, John R. Olson, Stephen L. Klobucar, Karen E. Mock
Ecology Center Publications
The North Slope of Alaska contains arctic fish populations that are important for subsistence of local human populations, and are under threat from natural resource extraction and climate change. We designed and evaluated four quantitative PCR assays for the detection of environmental DNA from five Alaskan fish species present on the North Slope of Alaska: burbot (Lota lota), arctic char (Salvelinus alpinus), Dolly Varden (Salvelinus malma), arctic grayling (Thymallus arcticus), and slimy sculpin (Cottus cognatus). All assays were designed and tested for species specificity and sensitivity, and all assays detected …