Other Life Sciences Commons^™

Comparison Of A One-Step Real-Time Rt-Pcr And A Nested Real-Time Rt-Pcr For A Genogroup Ii Norovirus Reveals Differences In Sensitivity Depending Upon Assay Design And Visualization, Clyde S. Manuel, Cassandra Suther, Matthew D. Moore, Lee-Ann Jaykus

Food Science Department Faculty Publication Series

Human norovirus (NoV) is the leading cause of acute viral gastroenteritis and a major source of foodborne illness. Detection of NoV in food and environmental samples is typically performed using molecular techniques, including real-time reverse transcription polymerase chain reaction (RT-PCR) and less frequently, nested real-time PCR. In this study, we conducted a controlled comparison of two published NoV detection assays: a broadly reactive one-step real-time RT-PCR and a two-step nested real-time PCR assay. A 20% human fecal suspension containing a genogroup II human NoV was serially diluted, genome extracted, and subjected to amplification using the two assays compared via PCR …