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Full-Text Articles in Other Life Sciences

Attack And Defense Movements Involved In The Interaction Of Spodoptera Frugiperda And Helicoverpa Zea (Lepidoptera: Noctuidae), José P. F. Bentivenha, Edson L.L. Baldin, Débora G. Montezano, Thomas E. Hunt, Silvana L. Paula-Moraes Jan 2017

Attack And Defense Movements Involved In The Interaction Of Spodoptera Frugiperda And Helicoverpa Zea (Lepidoptera: Noctuidae), José P. F. Bentivenha, Edson L.L. Baldin, Débora G. Montezano, Thomas E. Hunt, Silvana L. Paula-Moraes

Department of Entomology: Faculty Publications

The fall armyworm, Spodoptera frugiperda (J. E. Smith) and the corn earworm, Helicoverpa zea (Boddie) are among the main pests of maize. Both species exhibit cannibalistic behavior and quite often share the same feeding guild in maize (maize ears), which can result in several interspecific and intraspecific interactions. Paired interaction scenarios of intraspecific and interspecific larvae were assessed in arenas in the presence and absence of food to characterize movements resulting from interactions of these insects. There was a difference in the frequency of behavioral movements in all the interactions, except for S. frugiperda in the presence of food. Head …


Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Marce D. Lorenzen, Richard W. Beeman, John E. Foster, Blair D. Siegfried Jan 2010

Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Marce D. Lorenzen, Richard W. Beeman, John E. Foster, Blair D. Siegfried

Department of Entomology: Faculty Publications

To test the efficacy of RNA interference (RNAi) as a method for target-site screening in Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model, Tribolium castaneum. Here the cloning of the D. v. vergifera orthologs of laccase 2 (DvvLac2) and chitin synthase 2 (DvvCHS2) is reported. Injection of DvvLac2-specific doublestranded RNA resulted in prevention of post-molt cuticular tanning, while injection of DvvCHS2- specific dsRNA reduced chitin levels in midguts. Silencing of both DvvLac2 and DvvCHS2 was confirmed by RT-PCR and quantitative RT-PCR. As in T. …


Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Richard W. Beeman, Marce D. Lorenzen, John E. Foster, Blair D. Siegfried Jan 2010

Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Richard W. Beeman, Marce D. Lorenzen, John E. Foster, Blair D. Siegfried

Department of Entomology: Faculty Publications

To test the efficacy of RNA interference (RNAi) as a method for target-site screening in Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model, Tribolium castaneum. Here the cloning of the D. v. vergifera orthologs of laccase 2 (DvvLac2) and chitin synthase 2 (DvvCHS2) is reported. Injection of DvvLac2-specific doublestranded RNA resulted in prevention of post-molt cuticular tanning, while injection of DvvCHS2- specific dsRNA reduced chitin levels in midguts. Silencing of both DvvLac2 and DvvCHS2 was confirmed by RT-PCR and quantitative RT-PCR. As in T. …