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Autoradiographic Localization Of Exogenously Supplied Amino Acids After Uptake By The Polychaete, Glycera Dibranchiata Ehlers, Paul Chien, Michael Rice

Michael A Rice

Localization of amino acid transport sites within the integument of Glycera dibranchiata Ehlers was carried out by freeze-section and freeze-dry histological techniques. The worms were exposed for one hour to the non-metabolizable amino acid analog c(14)-cycloleucine in seawater. Freeze-sectioning and freeze drying of tissue samples allows the localization of water soluble compounds by autoradiography. Amino acid uptake is largely across the parapodial gill surfaces and the epidermis. The gills become rapidly labelled with c(14)-cycloleucine to a greater extent than the epidermal cell layers. Coelomocytes within the gills become radiolabeled in one hour. It is suggested that the rapid labelling of …