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Full-Text Articles in Neuroscience and Neurobiology
Rapid Golgi Stain For Dendritic Spine Visualization In Hippocampus And Prefrontal Cortex, Maya Frankfurt, Rachel E. Bowman
Rapid Golgi Stain For Dendritic Spine Visualization In Hippocampus And Prefrontal Cortex, Maya Frankfurt, Rachel E. Bowman
Psychology Faculty Publications
Golgi impregnation, using the Golgi staining kit with minor adaptations, is used to impregnate dendritic spines in the rat hippocampus and medial prefrontal cortex. This technique is a marked improvement over previous methods of Golgi impregnation because the premixed chemicals are safer to use, neurons are consistently well impregnated, there is far less background debris, and for a given region, there are extremely small deviations in spine density between experiments. Moreover, brains can be accumulated after a certain point and kept frozen until further processing. Using this method any brain region of interest can be studied. Once stained and cover …
Neonatal Isolation Stress Inhibits Pre-Weaning Weight Gain And Mild-Stressor Induced Locomotor Activity In Early Adolescent Male And Female Rats, Peter Villavecchia, Mindy Miserendino
Neonatal Isolation Stress Inhibits Pre-Weaning Weight Gain And Mild-Stressor Induced Locomotor Activity In Early Adolescent Male And Female Rats, Peter Villavecchia, Mindy Miserendino
Psychology Faculty Publications
The present study looked at the effects of neonatal isolation, an early life stress experience, in male and female early adolescent rats, an age which is underrepresented in the early stress literature. Four stress-sensitive indices were assessed: weight gain during the pre-weaning period, open field activity, and locomotor activity in response to two mild stressors: exposure to a novel environment, and a single IP saline injection. Rats in the neonatal isolation condition were removed from dam and littermates on postnatal days 2-9 in accord with the procedure used by Kehoe et al. (1995); behavioral testing occurred on PN25-PN30 during …
Adolescent Bisphenol-A Exposure Decreases Dendritic Spine Density: Role Of Sex And Age, Rachel E. Bowman, Victoria N. Luine, Hameda Khandaker, Joseph J. Villafane, Maya Frankfurt
Adolescent Bisphenol-A Exposure Decreases Dendritic Spine Density: Role Of Sex And Age, Rachel E. Bowman, Victoria N. Luine, Hameda Khandaker, Joseph J. Villafane, Maya Frankfurt
Psychology Faculty Publications
Bisphenol-A (BPA), a common environmental endocrine disruptor, modulates estrogenic, androgenic, and anti-androgenic effects throughout the lifespan. We recently showed that low dose BPA exposure during adolescence increases anxiety and impairs spatial memory independent of sex. In the current study, six week old Sprague Dawley rats (n=24 males, n=24 females) received daily subcutaneous injections (40 µg/kg bodyweight) of BPA or vehicle for one week. Serum corticosterone levels in response to a 1 h restraint stress and spine density were examined at age 7 (cohort 1) and 11 (cohort 2) weeks. Adolescent BPA exposure did not alter stress dependent corticosterone responses but …
Sexually Dimorphic Effects Of Prenatal Stress On Cognition, Hormonal Responses, And Central Neurotransmitters, Rachel E. Bowman, Neil J. Maclusky, Yessenia Sarmiento, Maya Frankfurt, Marisa Gordon, Victoria N. Luine
Sexually Dimorphic Effects Of Prenatal Stress On Cognition, Hormonal Responses, And Central Neurotransmitters, Rachel E. Bowman, Neil J. Maclusky, Yessenia Sarmiento, Maya Frankfurt, Marisa Gordon, Victoria N. Luine
Psychology Faculty Publications
Exposure to stress during gestation results in physiological and behavioral alterations that persist into adulthood. This study examined the effects of prenatal stress on the postnatal expression of sexually differentiated cognitive, hormonal, and neurochemical profiles in male and female rats. Pregnant dams were subjected to restraint stress three times daily for 45 min during d 14-21 of pregnancy. The offspring of control and prenatally stressed dams were tested for anxiety-related and cognitive behaviors, stress and gonadal steroid hormone levels, as well as monoamines and metabolite levels in selected brain regions. Postnatal testosterone levels (measured at 1 and 5 d) did …