Microbiology Commons^™

Impacts Of Swine Manure Application And Alternative N-Management Practices On Productivity, Sustainability And Water Quality, Rameshwar S. Kanwar, Douglas Karlen, Cynthia A. Cambardella, Thomas Moorman, Thomas R. Steinheimer, Thomas S. Colvin

Douglas L Karlen

In the fourth year of this ongoing project, the effects of nine N-management practices under different tillage and cropping systems were evaluated. Forty experimental plots equipped with individual sumps and subsurface drainage metering and monitoring devices were used for the study. Overall results indicate that manure application rates and methods can be successfully managed for corn-soybean systems without damaging the water quality if the appropriate amount of N from swine manure can be applied.

Effect Of Tillage, Crop Rotation And Innovative Nitrogen And Pesticide Management Practices On Productivity, Sustainability And Water Quality, Rameshwar S. Kanwar, Douglas Karlen, Cynthia A. Cambardella, Thomas B. Moorman, T. R. Steinheimer, Thomas S. Colvin

Douglas L Karlen

Better nitrogen (N) management practices can improve nitrogen uptake and possibly reduce nitrogen leaching to groundwater. More efficient herbicide use can decrease or eliminate the herbicide leaching losses to water sources. In this project, the effects of seven N management practices on water quality were evaluated after collecting data from 40 experimental plots. Lower rates of N application and strip and hay cropping systems help produce lower concentrations of NO3-N in the shallow groundwater in comparison with the higher rate of N application. Also, banding of herbicides has resulted in lower herbicide losses to shallow groundwater. The use of the …

Method Assessment For Microalgae Quantification In Wastewater Treatment And Biofuel Production, Kristen S. Harrison

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

There is growing interest in the use of microalgae for wastewater treatment and biofuel production. However, there is not yet a reliable, user-friendly method of quantifying the biomass and lipid production of a large sample of algae. This study aims to establish and validate new methods for green microalgae quantification using the Microplate reader and the Coulter Counter. Chlorella vulgaris was chosen as the ‘ideal’ algal species for wastewater treatment and biofuel production due to its structure, lipid production, and pollution removal ability. Three traditional quantification methods -- microscope cell count, lipid separation, and chlorophyll extraction -- were compared to …

Production And Harvest Of Microalgae In Wastewater Raceways With Resource Recycling, Alexander Colin Roberts

Master's Theses

Microalgae can be grown on municipal wastewater media to both treat the wastewater and produce feedstock for algae biofuel production. However the reliability of treatment must be demonstrated, as well as high areal algae productivity on recycled wastewater media and efficient sedimentation harvesting. This processes was studied at pilot scale in the present research.

A pilot facility was operated with nine CO₂-supplemented raceway ponds, each with a 33-m² surface area and a 0.3-m depth, continuously from March 6, 2013 through September 24, 2014. The ponds were operated as three sets of triplicates with two sets continuously fed …

Promoting Extracellular Matrix Crosslinking In Synthetic Hydrogels, Marcos M. Manganare

Masters Theses

The extracellular matrix (ECM) provides mechanical and biochemical support to tissues and cells. It is crucial for cell attachment, differentiation, and migration, as well as for ailment-associated processes such as angiogenesis, metastases and cancer development. An approach to study these phenomena is through emulation of the ECM by synthetic gels constructed of natural polymers, such as collagen and fibronectin, or simple but tunable materials such as poly(ethylene glycol) (PEG) crosslinked with short peptide sequences susceptible to digestion by metalloproteases and cell-binding domains. Our lab uses PEG gels to study cell behavior in three dimensions (3D). Although this system fosters cell …

Pathogen Removal In Natural Wastewater Treatment And Resource Recovery Systems: Solutions For Small Cities In An Urbanizing World, Matthew Eric Verbyla

USF Tampa Graduate Theses and Dissertations

Sanitation, renewable energy, and food security are among the most pressing global development needs of the century, especially for small cities with rapid population growth. Currently, 53% of the world’s population either lacks access to improved sanitation or discharges fecal waste to the environment without treatment. Furthermore, 80% of food consumed in developing regions is produced by 500 million small farms, and while many of them are still rain-fed, irrigated agriculture is increasing. The post-2015 Sustainable Development Goals, recently adopted by the United Nations, include targets to address the water-energy-food nexus. Wastewater reuse in agriculture can be an important solution …

Seeps And Springs At A Platteville “Observatory” On The River Bluffs, Bj Bonin, Greg Brick, Julia R. Steenberg

Sinkhole Conference 2015

Residential building construction along the Mississippi River bluffs in the 1970s created a unique enclosed outcrop of the Late Ordovician Platteville Formation at Lilydale, Minnesota. This outcrop was examined in early 2013 after a newly-formed spring flooded an elevator shaft the previous year, drawing attention to the foundation conditions. The Lexington Riverside property is a six story condominium complex constructed within the top of the bluff. A two-level underground parking garage was built into the bluff. Bedrock was mechanically excavated to accommodate the construction of the building, creating an unweathered rock surface. The space between the structure and the excavated …

Characterization Of Antimicrobial Susceptibility Profile Of Biofield Treated Multidrug-Resistant Klebsiella Oxytoca, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Label-Free Surface-Enhanced Raman Spectroscopy-Linked Immunosensor Assay (Slisa) For Environmental Surveillance, Vinay Bhardwaj

FIU Electronic Theses and Dissertations

The contamination of the environment, accidental or intentional, in particular with chemical toxins such as industrial chemicals and chemical warfare agents has increased public fear. There is a critical requirement for the continuous detection of toxins present at very low levels in the environment. Indeed, some ultra-sensitive analytical techniques already exist, for example chromatography and mass spectroscopy, which are approved by the US Environmental Protection Agency for the detection of toxins. However, these techniques are limited to the detection of known toxins. Cellular expression of genomic and proteomic biomarkers in response to toxins allows monitoring of known as well as …

The Contribution Of Syntrophic Fatty-Acid Degrading Microbial Communities To Anaerobic Digester Function And Stability, Prince Peter Mathai

Dissertations (1934 -)

Anaerobic digestion (AD), the conversion of complex organic matter to methane, occurs through a series of reactions mediated by different guilds of microorganisms. AD process imbalances, such as organic overload or high organic loading rates (OLR), can result in the accumulation of volatile fatty acids (VFA) e.g., propionate, which must be degraded to maintain stable reactor function. VFAs are metabolized by syntrophic fatty-acid degrading bacteria (SFAB) in association with methanogenic archaea (collectively, syntrophic microbial communities, SMC). Despite their indispensable role in AD, little is known about the ecology of SFAB, especially under stressed conditions. To facilitate ecological studies, four quantitative …

A Novel Synthetic Yeast For Enzymatic Biodigester Pretreatment, Tianyu Tan, Mark S. Aronson, Arren Liu, Jill H. Osterhus, Melissa Robins, Suraj Mohan, Erich Leazer, Bowman Clark, Alexa Petrucciani, Katherine Lowery, James Welch, Casey Martin, Helena Lysandrou, Michael E. Scharf, Jenna Rickus

The Summer Undergraduate Research Fellowship (SURF) Symposium

Lignin, a complex organic polymer, is a major roadblock to the efficiency of biofuel conversion as it both physically blocks carbohydrate substrates and poisons biomass degrading enzymes, even if broken down to monomer units. A pretreatment process is often applied to separate the lignin from biomass prior to biofuel conversion. However, contemporary methods of pretreatment require large amounts of energy, which may be economically uncompelling or unfeasible. Taking inspiration from several genes that have been isolated from termites and fungi which translate to enzymes that degrade lignin, we want to establish a novel “enzymatic pretreatment” system where microbes secrete these …

Field Scale Application Of Nanoscale Zero Valent Iron: Mobility, Contaminant Degradation, And Impact On Microbial Communities, Chris M.D. Kocur

Electronic Thesis and Dissertation Repository

This thesis began by verifying that nanoscale zero valent iron (nZVI) synthesis methods could be scaled up and implemented at the field scale in a safe manner. This led to successful demonstration of nZVI injection and mobility under constant head gravity injection into a contaminated utility corridor in Sarnia, Ontario. Where field studies have fallen short in the past was linking the somewhat qualitative field geochemical parameters to other evidence of nZVI transport. Definitive nZVI detection was elusive in previous field studies due to the highly reactive nature of the particles caused by their high surface area. nZVI was detected …

Simulation And Validation Of Radio Frequency Heating Of Shell Eggs, Soon Kiat Lau

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Finite element models were developed with the purpose of finding an optimal radio frequency (RF) heating setup for pasteurizing shell eggs. Material properties of the yolk, albumen, and shell were measured and fitted into equations that were used as inputs for the model. When the egg was heated by itself, heating tend to be focused at the air cell to result in a “coagulation ring.” The focused heating near the air cell of the egg prevented satisfactory pasteurization of the egg, but deeper analysis of the simulation results offered a new perspective on how non-uniform RF heating could occur in …

Effect Of Biofield Treatment On Structural And Morphological Properties Of Silicon Carbide, Mahendra Kumar Trivedi, Gopal Nayak, Rama Mohan Tallapragada, Shrikant Patil, Omprakash Latiyal, Snehasis Jana

Mahendra Kumar Trivedi

Phenotypic And Biotypic Characterization Of Klebsiella Oxytoca: An Impact Of Biofield Treatment, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana

Mahendra Kumar Trivedi

Deletion Of Nfnab In Thermoanaerobacterium Saccharolyticum And Its Effect On Metabolism, Jonathan Lo, Tianyong Zheng, Daniel G. Olson, Natalie Ruppertsberger, Shital Tripathi, Adam Guss, Lee Lynd

Dartmouth Scholarship

NfnAB catalyzes the reversible transfer of electrons from reduced ferredoxin and NADH to 2 NADP⁺. The NfnAB complex has been hypothesized to be the main enzyme for ferredoxin oxidization in strains of Thermoanaerobacterium saccharolyticum engineered for increased ethanol production. NfnAB complex activity was detectable in crude cell extracts of T. saccharolyticum. Activity was also detected using activity staining of native PAGE gels. The nfnAB gene was deleted in different strains of T. saccharolyticum to determine its effect on end product formation. In wild-type T. saccharolyticum, deletion of nfnAB resulted in a 46% increase in H₂ formation but …

Cofactor Specificity Of The Bifunctional Alcohol And Aldehyde Dehydrogenase (Adhe) In Wild-Type And Mutant Clostridium Thermocellum And Thermoanaerobacterium Saccharolyticum, Tianyong Zheng, Daniel G. Olson, Liang Tian, Yannick J. Bomble, Michael Himmel, Jonathan Lo, Shuen Hon, A. Joe Shaw, Johannes P. Van Dijken, Lee Lynd

Dartmouth Scholarship

Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are thermophilic bacteria that have been engineered to produce ethanol from the cellulose and hemicellulose fractions of biomass, respectively. Although engineered strains of T. saccharolyticum produce ethanol with a yield of 90% of the theoretical maximum, engineered strains of C. thermocellum produce ethanol at lower yields (∼50% of the theoretical maximum). In the course of engineering these strains, a number of mutations have been discovered in their adhE genes, which encode both alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) enzymes. To understand the effects of these mutations, the adhE genes from six strains of C. …

Applications Of Microbial Desalination And Photocatalytic Disinfection For The Removal Of Contaminants In Drinking Water, Kristen Shirlee-Ann Brastad

Theses and Dissertations

Trends in drinking water treatment in recent years have been moving toward the use of membrane separation in order to reduce contaminants in water. There are many forms of membrane separation technology such as ultrafiltration, nanofiltration, microfiltration, reverse osmosis, and extruded ion exchange membranes. These membranes have many different applications and may be used to remove many materials from water such as salts, viruses and bacteria, selectively remove cations or anions, or remove organics.

Microbial desalination cells (MDCs) are an emerging concept which use bioelectric potential produced from organics via microbial metabolism to accomplish desalination. MDCs consist of three compartments, …

The Bifunctional Alcohol And Aldehyde Dehydrogenase Gene, Adhe, Is Necessary For Ethanol Production In Clostridium Thermocellum And Thermoanaerobacterium Saccharolyticum, Jonathan Lo, Tianyong Zheng, Shuen Hon, Daniel G. Olson, Lee Lynd

Dartmouth Scholarship

Thermoanaerobacterium saccharolyticum and Clostridium thermocellum are anaerobic thermophilic bacteria being investigated for their ability to produce biofuels from plant biomass. The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is present in these bacteria and has been known to be important for ethanol formation in other anaerobic alcohol producers. This study explores the inactivation of the adhE gene in C. thermocellum and T. saccharolyticum. Deletion of adhE reduced ethanol production by >95% in both T. saccharolyticum and C. thermocellum, confirming that adhE is necessary for ethanol formation in both organisms. In both adhE deletion strains, fermentation products shifted from ethanol …

Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt

Department of Civil and Environmental Engineering: Faculty Publications

The use of wastewater for irrigation may introduce antimicrobials and human pathogens into the food supply through vegetative uptake. The objective of this study was to investigate the uptake of three antimicrobials and Salmonella in two lettuce cultivars. After repeated subirrigation with synthetic wastewater, lettuce leaves and soil were collected at three sequential harvests. The internalization frequency of Salmonella in lettuce was low. A soil horizon-influenced Salmonella concentration gradient was determined with concentrations in bottom soil 2 log CFU/g higher than in top soil. Lincomycin and sulfamethoxazole were recovered from lettuce leaves at concentrations as high as 822 ng/g and …

Exploratory Study Of Seasonal Indoor Bioaerosols And The Associated Health Outcomes In Low-Income Communities In El Paso, Texas, 2014, Eric Martinez

Open Access Theses & Dissertations

Introduction: Structural degradation, moisture levels, and building failures can influence poor indoor air quality and create a good environment for bioaerosols. People spend much of their time indoors and can potentially be exposed to these bioaerosols. Several studies have correlated exposures to high concentrations of bioaerosols to various health problems, severe allergies, asthma, or other respiratory illness. Objectives: The objectives of this study are to describe bioaerosol concentrations, meteorological factors, and health outcomes during the summer and fall seasons; characterize bioaerosols (bacteria and fungi) collected in low-income communities; compare bioaerosol concentrations by season; compare meteorological factors to bioaerosol concentrations by …

Integrated Nanoscale Imaging And Spatial Recognition Of Biomolecules On Surfaces, Congzhou Wang

Theses and Dissertations

Biomolecules on cell surfaces play critical roles in diverse biological and physiological processes. However, conventional bulk scale techniques are unable to clarify the density and distribution of specific biomolecules in situ on single, living cell surfaces at the micro or nanoscale. In this work, a single cell analysis technique based on Atomic Force Microscopy (AFM) is developed to spatially identify biomolecules and characterize nanomechanical properties on single cell surfaces. The unique advantage of these AFM-based techniques lies in the ability to operate in situ (in a non-destructive fashion) and in real time, under physiological conditions or controlled micro-environments.

First, AFM-based …