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Articles 1 - 6 of 6
Full-Text Articles in Microbiology
Fungal Solid-State Fermentation And Various Methods Of Enhancement In Cellulase Production, Li Wan Yoon, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Fungal Solid-State Fermentation And Various Methods Of Enhancement In Cellulase Production, Li Wan Yoon, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Teck Nam Ang Dr.
Cellulase serves vast applications in the industries of biofuel, pulp and paper, detergent and textile. With the presence of its three components i.e. endoglucanase, exoglucanase and β-glucosidase, the enzyme can effectively depolymerize the cellulose chains in lignocellulosic substrate to produce smaller sugar units that consists of cellobiase and glucose. Fungi are the most suitable cellase producers attributing to its ability to produce a complete cellulase system. Solid state fermentation (SSF) by fungi is a preferable production route for cellulase as it imposes lower cost and enables the production of cellulase with higher titre. This article gives an overview on the …
An Efficient Biotreatment Process For Polyvinyl Alcohol Containing Textile Wastewater, Sandip S. Magdum, Gauri P. Minde, Upendra S. Adhyapak, Kalyanraman V.
An Efficient Biotreatment Process For Polyvinyl Alcohol Containing Textile Wastewater, Sandip S. Magdum, Gauri P. Minde, Upendra S. Adhyapak, Kalyanraman V.
Sandip S. Magdum
The aim of this work was to optimize the biodegradation of polyvinyl alcohol (PVA) containing actual textile wastewater for a sustainable treatment solution. The isolated microbial consortia of effective PVA degrader namely Candida Sp. and Pseudomonas Sp., which were responsible for symbiotic degradation of chemical oxidation demand (COD) and PVA from desizing wastewater. In the process optimization, the maximum aeration was essential to achieve a high degradation rate, where as stirring enhances further degradation and foam control. Batch experiments concluded with the need of 16 lpm/l and 150 rpm of air and stirring speed respectively for high rate of COD …
Development Of A Novel Inoculum Preparation Method For Solid-State Fermentation - Cellophane Film Culture (Cfc) Technique, Teck Nam Ang, Gek Cheng Ngoh, Adeline Seak May Chua
Development Of A Novel Inoculum Preparation Method For Solid-State Fermentation - Cellophane Film Culture (Cfc) Technique, Teck Nam Ang, Gek Cheng Ngoh, Adeline Seak May Chua
Teck Nam Ang Dr.
This study reports a user-friendly technique in the preparation of fungal inoculum intended for solid-state fermentation (SSF) – Cellophane Film Culture (CFC) technique. This technique uses cellophane film-overlaid agar plates to facilitate the separation of fungal biomass. The findings showed that inoculum of P. sajor-caju produced is viable, and it was confirmed by the presence of laccase enzyme activity in SSF of rice husk. The correlation between fungal dry and wet weights (r2 = 0.9329) provides an accurate estimation of fungal dry weight from its wet weight during inoculum preparation. Besides, this technique does not require a strict sterile handling …
Applied And Mechanistic Studies Of Microbial 17beta-Estradiol Degradation, Zhongtian Li
Applied And Mechanistic Studies Of Microbial 17beta-Estradiol Degradation, Zhongtian Li
Z Li
The presence of natural estrogens, a class of endocrine disrupting compounds, in water has caused increasing concerns over their adverse impacts on the health of aquatic eco-systems and human beings. In this study, adsorption characteristics of two natural estrogens, 17β-estradiol (E2) and estrone (E1), on granular activated carbon (GAC) were investigated in isotherm tests and in a GAC column. The GAC column was then converted to a biologically active carbon (BAC) column and the removal efficiency of E2 and its primary biodegradation intermediate E1 were monitored. During BAC operation, the impacts of various reactor operation parameters, such as the carbon …
Proteomic Analysis Of 17Β-Estradiol Degradation By Stenotrophomonas Maltophilia, Zhongtian Li
Proteomic Analysis Of 17Β-Estradiol Degradation By Stenotrophomonas Maltophilia, Zhongtian Li
Z Li
Microbial degradation plays a critical role in determining the environmental fate of steroid hormones, such as 17β-estradiol (E2). The molecular mechanisms governing the microbial transformation of E2 and its primary degradation intermediate, estrone (E1), are largely unknown. The objective of this study was to identify metabolism pathways that might be involved in microbial estrogen degradation. To achieve the objective, Stenotrophomonas maltophilia strain ZL1 was used as a model estrogen degrading bacterium and its protein expression level during E2/E1 degradation was studied using quantitative proteomics. During an E2 degradation experiment, strain ZL1 first converted E2 to E1 stoichiometrically. At 16 h …
A Quantitative Method For Fungal Ligninolytic Enzyme, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
A Quantitative Method For Fungal Ligninolytic Enzyme, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Teck Nam Ang Dr.
Screening is conducted to select a fungus with desired characteristics intended for various applications, e.g. bioremediation and enzyme production. A qualitative method was used in this study for screening of fungal ligninolytic enzymes activities. The fungal ligninolytic activity was correlated with its growth from the screenings to identify a suitable fungus for solid substrate fermentation. Four strains of fungi, namely Phanerochaete chrysosporium, Pycnoporus sanguineus, Phlebia radiata and Pleurotus sajor-caju, were screened for their ligninolytic enzymes activities using guaiacol and Remazol Brilliant Blue-R (RBB-R) as screening reagents. The screenings were conducted at both the optimal growth temperature of each fungus and …