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Full-Text Articles in Microbiology
Characterization Of Fusobacterium Isolates From The Respiratory Tract Of White-Tailed Deer (Odocoileus Virginianus ), Amit Kumar, Dvm, Ms, Phd
Characterization Of Fusobacterium Isolates From The Respiratory Tract Of White-Tailed Deer (Odocoileus Virginianus ), Amit Kumar, Dvm, Ms, Phd
Amit Kumar, DVM, MS, PhD
A total of 23 clinical isolates of Fusobacterium spp. were recovered at necropsy over a 2-year period from the respiratory tract of white-tailed deer (Odocoileus virginianus). Isolates were identified as Fusobacterium varium (18/23),Fusobacterium necrophorum subsp. funduliforme (3/23), and Fusobacterium necrophorum subsp. necrophorum (2/23). Usingpolymerase chain reaction–based detection of virulence genes, all F. necrophorum isolates were positive for the promoter region of the leukotoxin operon and the hemagglutinin-related protein gene, while all F. varium isolates were negative. The presence of the leukotoxin gene in F. necrophorum isolates and the absence of this gene in F. varium isolates were confirmed by Southern …
Adhesion Of Fusobacterium Necrophorum To Bovine Endothelial Cells Is Mediated By Outer Membrane Proteins, Amit Kumar
Adhesion Of Fusobacterium Necrophorum To Bovine Endothelial Cells Is Mediated By Outer Membrane Proteins, Amit Kumar
Amit Kumar, DVM, MS, PhD
Fusobacterium necrophorum, a Gram-negative anaerobe, is frequently associated with suppurative and necrotic infections of animals and humans. The organism is a major bovine pathogen, and in cattle, the common fusobacterial infections are hepatic abscesses, foot rot, and necrotic laryngitis. The species comprises two subspecies: F. necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme. Bacterial adhesion to the host cell surface is a critical initial step in the pathogenesis, and outer membrane proteins (OMP) play an important role in adhesion and establishment of certain Gram-negative bacterial infections. The means by which F. necrophorum attaches to epithelial or endothelial cells has not …
Production Of Nitrogen-Based Platform Chemical: Cyanophycin Biosynthesis Using Recombinant Escherichia Coli And Renewable Media Substitutes, Amit Kumar
Amit Kumar, DVM, MS, PhD
BACKGROUND:Cyanophycin (CGP) is a sustainable polymer that can be converted to a derivative with reduced arginine content, or to completely biodegradable poly-aspartic acid, which can substitute for non-biodegradable polyacrylates. In nature, it is produced by most cyanobacteria; however, these microbes are not suitable for large-scale production due to slow growth and low polymer content. RESULTS: Cyanophycin synthetase gene (cphA) from Anabaena variabilis ATCC 29413 was PCR amplified and cloned into Escherichiacoli.Differentrenewablemediacomponents,suchassoybeanmeal,potatowastesandcorn-derivedzein hydrolysate, were evaluated for their feasibility for CGP production at shake flask level. The optimized conditions were then tested in a 7 L bioreactor; a maximum cell weight of …
Characterization Of Fusobacterium Necrophorum Isolated From Llama And Alpaca, Amit Kumar
Characterization Of Fusobacterium Necrophorum Isolated From Llama And Alpaca, Amit Kumar
Amit Kumar, DVM, MS, PhD
Fusobacterium necrophorum, a Gram-negative, anaerobic bacterium, is an opportunistic animal and human pathogen that causes a variety of infections termed necrobacillosis. There are 2 subspecies of F. necrophorum (subsp. necrophorum and subsp. funduliforme) that differ morphologically and biochemically and in virulence. Leukotoxin, a secreted protein, is considered to be the major virulence factor. In camelids, F. necrophorum causes a variety of infections, generally involving the lips, tongue, pharynx, interdigital spaces, foot pad, larynx, mandible, or maxillary bones. The objective of the current study was to characterize the presumptive Fusobacterium isolates from a variety of necrotic infections in llama (Lama glama) …
Outer Membrane Proteins Of Fusobacterium Necrophorum, Amit Kumar
Outer Membrane Proteins Of Fusobacterium Necrophorum, Amit Kumar
Amit Kumar, DVM, MS, PhD
Fusobacterium necrophorum, classified into subsp. necrophorum (Fnn) and subsp. funduliforme (Fnf), is frequently associated with necrotic infections of animals and humans. The outer membrane proteins (OMP) of many Gram negative bacteria play an important role in bacterial adhesion and establishment of infection. The OMP profile of F. necrophorum has not been well characterized. We analyzed OMP of bovine strains of Fnn and Fnf and human strains of F. necrophorum. Electrophoretic separations of extracted OMP of Fnn and Fnf strains of cattle showed a total of 19 and 20 protein bands, respectively. The most prominent protein band was 40 kDa in …
Composition And Methods For Detecting, Treating, And Protecting Against Fusobacterium Infection. United States Patent Application Pct/Us2012/030576 Filed March 26, 2012, Amit Kumar, Dvm, Ms, Phd
Composition And Methods For Detecting, Treating, And Protecting Against Fusobacterium Infection. United States Patent Application Pct/Us2012/030576 Filed March 26, 2012, Amit Kumar, Dvm, Ms, Phd
Amit Kumar, DVM, MS, PhD
A protein that helps Fusobacterium necrophorum to attach to eukaryotic host cells has been identified. The protein has been shown to have potential to be a vaccine candidate in in vitro and in vivo experiments.
Catecholamines Increase Conjugative Gene Transfer Between Enteric Bacteria., Amit Kumar
Catecholamines Increase Conjugative Gene Transfer Between Enteric Bacteria., Amit Kumar
Amit Kumar, DVM, MS, PhD
The ability of pathogenic bacteria to sense and respond to periods of host stress is critical to their lifestyle. Adrenaline and norepinephrine are catecholamines that mediate acute host stress in vertebrates and invertebrates. Catecholamines are also used as environmental cues to enhance growth, motility and virulence of bacterial pathogens via specific binding receptors. Incidence of multidrug resistant and highly virulent bacterial pathogens is on the rise, and majority of the genes for antimicrobial resistance (AMR) and virulence are carried on horizontally transferable genetic elements. Conjugation machinery offers an efficient method for acquisition of AMR and virulence genes, which may be …