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Full-Text Articles in Microbiology
Microbial Communities Hosted By Carnivorous Pitcher Plants: Diversity, Recruitment, Functions And Succession In Sarracenia Purpurea Microbiomes, Jacob Jeffrey Grothjan
Microbial Communities Hosted By Carnivorous Pitcher Plants: Diversity, Recruitment, Functions And Succession In Sarracenia Purpurea Microbiomes, Jacob Jeffrey Grothjan
Theses and Dissertations
The pitcher plant Sarracenia purpurea supplements nutrient acquisition through carnivory, capturing insect prey which are digested by a food web community of eukaryotes and bacteria. Analysis of both bacterial and eukaryotic diversity, and an understanding of bacterial recruitment into pitchers and succession of bacterial and eukaryotic communities over time have not been well explored. This thesis presents three studies designed to address these gaps using field sampling and manipulative greenhouse experiments. Study I compared bacterial and eukaryotic composition and diversity of pitcher communities within and between populations of plants in two distinct wetland habitats. Genetic sequence analysis revealed an underappreciated …
The Study Of The Phytopathogen Dickeya Dadantii 3937 Cpx Signaling On The Regulation Of Virulence And Antimicrobial Resistance, Daqing Jiang
The Study Of The Phytopathogen Dickeya Dadantii 3937 Cpx Signaling On The Regulation Of Virulence And Antimicrobial Resistance, Daqing Jiang
Theses and Dissertations
Bacteria respond to environmental cues through a variety of mechanisms. Two-component systems (TCSs) are a conserved mechanism used by bacteria to accurately respond and sense environmental changes. The monitoring of envelope perturbance is linked to TCS CpxA/CpxR in animal-infecting pathogens. The study of the TCS response regulator (RR) CpxR is largely unexplored in phytopathogens. This work focuses on the genetic linkage between cpxR and T3SS expression regulation. We identified the multiple roles of CpxR on several T3SS regulators and its participation in the bacterial second messenger (c-di-GMP) signaling cascade. Moreover, a compound library screening revealed a novel Cpx inducer CHIR-090, …
Roles Of Secreted Proteins And Iron Utilization Proteins In Virulence Of The Fish Pathogen Flavobacterium Columnare, Rachel Conrad
Roles Of Secreted Proteins And Iron Utilization Proteins In Virulence Of The Fish Pathogen Flavobacterium Columnare, Rachel Conrad
Theses and Dissertations
The Gram-negative bacterium Flavobacterium columnare is the causative agent of columnaris disease and is responsible for significant economic losses in aquaculture. Little is known regarding the virulence factors produced by F. columnare, and control measures are limited. Like many members of the phylum Bacteroidetes, F. columnare uses the type IX secretion system (T9SS) to secrete enzymes, adhesins, and proteins involved in gliding motility. When a core component of this system was deleted in the wild type strain, the resulting mutant was avirulent in zebrafish, rainbow trout, and channel catfish infection studies. This suggests that the individual secreted proteins may function …