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Bacteriophages | Staphylococcus | Bacteria -- Adhesion | Udder -- Diseases | Dairy cattle -- Diseases | Udder -- Microbiology | Anti-infective agents | Drug Resistance
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Full-Text Articles in Microbiology
Purification And Applications Of A Bacteriophage-Derived Enzyme Chapk Against Problematic Staphylococci., Niamh Marie O'Shea
Purification And Applications Of A Bacteriophage-Derived Enzyme Chapk Against Problematic Staphylococci., Niamh Marie O'Shea
Theses
The bacteriophage K-derived peptidase CHAPk (Cysteine/Histidine-dependant amidohydrolase peptidase) can be produced from E. coli XL 1-Blue. Standard operating procedures for the production, purification and quality assessment of the peptidase were developed and set out in detail in this thesis. CHAPk exhibits strong antistaphylococcal properties and the purified enzyme was evaluated for its potential to eliminate S. aureus from the bovine udder and from human skin. As a teat dip CHAPk reduced the levels of S. aureus by three to five log cycles on both animal and human surfaces. CHAPk activity in milk was evaluated using challenge assays against the bovine …