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Full-Text Articles in Microbiology
Β-Lactamase: Evolution Of Extended Spectrum Enzymes, Priscilla Shine
Β-Lactamase: Evolution Of Extended Spectrum Enzymes, Priscilla Shine
Theses
β-Lactam antibiotics are commonly used antimicrobial agents in clinical medicine. Because of their frequent use it is perhaps not surprising that resistance to these agents has increased over the past few decades, thus compromising the therapeutic advantages offered by these agents. The most common cause of resistance to β-lactam-based antibiotics has been the evolution of a group of enzymes known as β-lactamases, which act by inactivating the drug through structural modification. Plasmid-encoded TEM β-lactamases are examples of these hydrolytic enzymes and are associated with the amoxicillin resistance reported in over 50% of all E. coli isolates.
More recently β-lactamase enzymes …
The Investigation Of Rna Viruses Using The Atomic Force Microscope, Michelle Moloney
The Investigation Of Rna Viruses Using The Atomic Force Microscope, Michelle Moloney
Theses
The subject of viral entry into, and subsequent progeny virus egress, from cultured cells, has been extensively studied using numerous scientific techniques in fields ranging from cell culture to biochemistry to microscopy. The atomic force microscope (AFM) is a novel, developing instrument with unprecedented capabilities and this study was undertaken to observe enveloped Semliki Forest virus (SFV) and non-enveloped Theiler's Murine Encephalomyelitis virus (TMEV) entry into and egress from cultured cells using the AFM. Virus particle structure of both enveloped and non-enveloped viruses was also directly examined.
Cytopathic effect studies revealed that TMEV's are unable to produce progeny viruses in …