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Full-Text Articles in Microbiology
The Full Genome Sequence Of An Antarctic Microbe Constructed Using A Rapid, Portable Sequencer And A Hybrid Assembly, Bruce Wyatt Boles
The Full Genome Sequence Of An Antarctic Microbe Constructed Using A Rapid, Portable Sequencer And A Hybrid Assembly, Bruce Wyatt Boles
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Microbial genomes contain combinations of nucleotides that code for genes which subsequently determines the function of the cell. This information provides significant insight into the unique niche of microbes from extreme environments. Here we use two sequencing technologies, the Oxford Nanopore MinION with accompanying software and Illumina HiSeq, to generate a hybrid genome for a Shewanella strain isolated from an Antarctic glacier. Bioinformatic programs, Albacore and SPAdes, allowed us to decrease the time of genome assembly while also obtaining a large quantity of information related to the Shewanella sp. Our combined approach yielded a high quality genome assembly 5.3 …
What’S For Dinner? Different Carbon Compounds Influence Host Metabolism In A Model Roseobacter-Roseophage System, Kaylee Rae Jacobs
What’S For Dinner? Different Carbon Compounds Influence Host Metabolism In A Model Roseobacter-Roseophage System, Kaylee Rae Jacobs
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Viruses that infect bacteria (temperate phages) engage in complex interactions with their hosts. These phages can have two life cycles: lytic and lysogenic. For the latter, the phage can integrate its genome into that of its host and harmlessly replicate alongside with it. Conversely, upon induction, these integrated viruses can excise from their host genome and initiate the lytic cycle. A current paradigm is that phage induction is in response to host cell stress. However, in the absence of stressors, a low level of induction in a population occurs. This poorly understood phenomenon is referred to as spontaneous prophage induction …
Microbe Hunters: Searching For Anammox Bacteria In The Tennessee Aquarium, Claire Elbon
Microbe Hunters: Searching For Anammox Bacteria In The Tennessee Aquarium, Claire Elbon
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Ammonium and nitrite are toxic metabolic waste products generated by aquatic macroorganisms. They are of particular concern in closed systems, such as commercial aquaria. Typically, biological filtration systems are employed to regulate levels of toxic N species as they are more cost-efficient compared to water removal and replacement. Microbial communities that reside in these systems play vital roles in transformation of toxic N species. Commonly, nitrite and ammonium are converted into nitrate via nitrification. However, even nitrate is toxic at higher concentrations. Bacteria belonging to the phylum Planctomycetes can transform ammonium and nitrite to N2 via anaerobic ammonium oxidation (anammox). …
Ultramicrobacteria Genome Database Project, Abdullah A. Salim, Tien Tran, Andrew Putt, Terry C. Hazen
Ultramicrobacteria Genome Database Project, Abdullah A. Salim, Tien Tran, Andrew Putt, Terry C. Hazen
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Ultramicrobacteria (UMB) are a largely uncultured, globally abundant, and metabolically active group of bacteria. UMB have cell diameters ≤0.3μm, cell volumes ≤0.1 μm3, and small streamlined genomes. Recent findings indicate that UMB aid in bioremediation and nutrient cycling, but future investigations and comprehension of current findings are skewed by highly variable nomenclature and a lack of databases for functional, genomic, geochemical, or spatial data specific to candidate UMB. We aim to develop a user-friendly open-access database of various UMB candidates linked to an open-access online map where researchers can gather genomic, spatial, and geochemical data. Our comprehensive review of literature …