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Articles 1 - 17 of 17
Full-Text Articles in Microbiology
Quantatative Analysis Of Microbial Abundance Within Arctic Fjord Sediments Assessed Through Direct Counting, Alex Taylor Swystun
Quantatative Analysis Of Microbial Abundance Within Arctic Fjord Sediments Assessed Through Direct Counting, Alex Taylor Swystun
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Microbes found in the marine sediments are responsible for the production of nearly half of the carbon dioxide found in the atmosphere (Arrigo, 2005). The fjords of Svalbard (79°N) are not considered typical marine sediments because high iron content influences unique subsurface redox chemistry. Radiotracer studies have shown that these sediments contain active bacterial sulfate-reducing communities (Finke et al., 2016). In addition to bacteria, archaeal cells within these sediments have been in aggregates encompassed by sulfate-reducing bacteria (Ravenschlag et al., 2001). These anaerobic organisms participate in mediating environmental biogeochemical cycles, including the oxidation of methane (Ravenschlag et al., 2001) and …
Evidence For The Priming Effect In Single Strain And Simplified Communities Of Estuarine Bacteria, Abigail Amina Edwards
Evidence For The Priming Effect In Single Strain And Simplified Communities Of Estuarine Bacteria, Abigail Amina Edwards
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Through their transformation of naturally occurring organic matter, coastal marine bacteria play an essential role in carbon cycling. A phenomenon termed the priming effect (PE) occurs when microbial communities remineralize recalcitrant organic matter faster in the presence of labile organic matter and may be prevalent in coastal systems. To understand how microbial community members interact to induce PE, it is essential to first understand the mechanisms underlying PE in single strains and simplified bacterial communities. The effect to which different concentrations and sources of labile carbon stimulated the production of bacterial biomass from riverine organic matter by two marine bacteria …
Bioinformatic And Experimental Approaches For Deeper Metaproteomic Characterization Of Complex Environmental Samples, Ramsunder Mahadevan Iyer
Bioinformatic And Experimental Approaches For Deeper Metaproteomic Characterization Of Complex Environmental Samples, Ramsunder Mahadevan Iyer
Doctoral Dissertations
The coupling of high performance multi-dimensional liquid chromatography and tandem mass spectrometry for characterization of microbial proteins from complex environmental samples has paved the way for a new era in scientific discovery. The field of metaproteomics, which is the study of protein suite of all the organisms in a biological system, has taken a tremendous leap with the introduction of high-throughput proteomics. However, with corresponding increase in sample complexity, novel challenges have been raised with respect to efficient peptide separation via chromatography and bioinformatic analysis of the resulting high throughput data. In this dissertation, various aspects of metaproteomic characterization, including …
Investigating The Regulation Of Indole-3-Acetic Acid Production By The Plant Associated Microbe Pantoea Sp. Yr343, Kasey Noel Estenson
Investigating The Regulation Of Indole-3-Acetic Acid Production By The Plant Associated Microbe Pantoea Sp. Yr343, Kasey Noel Estenson
Doctoral Dissertations
The auxin indole-3-acetic acid (IAA) plays a central role in plant growth and development and many plant-associated microbes produce IAA. Several IAA biosynthetic pathways have been identified in microbes which use the precursor tryptophan. Pantoea sp. YR343, which was isolated from the Populus deltoides rhizosphere, is a robust plant root colonizer that produces IAA. Using genomic and metabolomics analyses, we predicted that the indole-3-pyruvate (IPA) pathway is the major pathway in Pantoea sp. YR343 for IAA production. To better understand IAA biosynthesis and the effects of IAA exposure on cell physiology, we performed proteomics on Pantoea sp. YR343 grown in …
Design And Synthesis Of Analogs Of Myo-Inositol, Serine, And Cysteine To Enable Chemical Biology Studies, Tanei J. Ricks
Design And Synthesis Of Analogs Of Myo-Inositol, Serine, And Cysteine To Enable Chemical Biology Studies, Tanei J. Ricks
Doctoral Dissertations
Phosphorylated myo-inositol compounds including inositol phosphates (InsPs) as well as the phosphatidylinositol polyphosphate lipids (PIPns) are critical biomolecules that regulate many of the most important biological processes and pathways. They are aberrant in many disease states due to their regulatory function. The same is true of the phospholipid phosphatidylserine (PS) which can serve as a marker to begin apoptosis. However, the full scope of activities of these structures is not clear, particularly since techniques that enable global detection and analysis of the production of these compounds spatially and temporally are lacking. With all of these obstacles in …
Systematics And Biogeography Of The Cortinarius Violaceus Group And Sequestrate Evolution In Cortinarius (Agaricales), Emma Harrower
Systematics And Biogeography Of The Cortinarius Violaceus Group And Sequestrate Evolution In Cortinarius (Agaricales), Emma Harrower
Doctoral Dissertations
Phylogenetics is a powerful tool used for illuminating the diversity of life on Earth, their evolution and their ecology. I created a multi-gene phylogenetic tree of Cortinarius section Cortinarius and uncovered five previously overlooked species, increasing the number of species in the section from seven to twelve. All members of the clade possess both cheilocystidia and pleurocystidia and possess a pigment known as (R)-39,49-dihydroxybphenylalanine. Ancestral state reconstruction estimated that the ancestral host was most likely an angiosperm, switching hosts when encountering novel host species in new lands, and only C. violaceus associating with the Pinaceae in North America. Biogeographic analysis …
Hybrid Recombineering: Crispr/Cas9 Mutagenesis Of Murine Cytomegalovirus Bac, Cody Sain
Hybrid Recombineering: Crispr/Cas9 Mutagenesis Of Murine Cytomegalovirus Bac, Cody Sain
Haslam Scholars Projects
Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that can asymptomatically infect immunocompetent hosts with little-to-no impact. Conversely, HCMV can trigger life-threatening disease in immunocompromised hosts such as neonates, organ transplant recipients, and people living with AIDS. HCMV is a unique pathogen in that it can hijack the immune system for its benefit and disseminates throughout the body using host immune cells. Previous studies suggest that innate immune cells are responsible for viral dissemination. However, adequate methods for investigating the spread of CMV within a murine model remain incomplete. We implicate the innate immune cell the neutrophil as a major cell …
Molecular Classification And Uv Tolerance Of Pigmented Antarctic Extremophiles, Aaron M. Perry
Molecular Classification And Uv Tolerance Of Pigmented Antarctic Extremophiles, Aaron M. Perry
Chancellor’s Honors Program Projects
No abstract provided.
Complete Denitrification By The Non-Denitrifier Anaeromyxobacter Dehalogenans: The Role Of Coupled Biotic-Abiotic Reactions, Jenny Rae Onley
Complete Denitrification By The Non-Denitrifier Anaeromyxobacter Dehalogenans: The Role Of Coupled Biotic-Abiotic Reactions, Jenny Rae Onley
Doctoral Dissertations
Nitrous oxide (N2O) is a potent greenhouse gas and ozone-depleting substance produced by many different pathways in the nitrogen cycle, including nitrification, denitrification, and chemodenitrification. Abiotic sources of N2O such as the chemical reaction between nitrite (NO2-) and ferrous ion (Fe[II]) are generally neglected in studies of N turnover in soils. Abiotic controls containing nitrate (NO3-) and ferric iron (Fe[III]) fail to capture potential reactions between intermediates of N cycle pathways (e.g., NO2- as an intermediate in NO3 - reduction to ammonium [NH4+] or …
Detection, Diversity, And Evolution Of Fungal Nitric Oxide Reductases (P450nor), Steven Adam Higgins
Detection, Diversity, And Evolution Of Fungal Nitric Oxide Reductases (P450nor), Steven Adam Higgins
Doctoral Dissertations
Nitrous oxide (N2O) is a gas responsible for significant ozone layer depletion and contributes to greenhouse effects in Earth’s atmosphere. N2O is primarily generated by denitrification, whereby nitrate (NO3-) or nitrite (NO2-) is converted to gaseous N2O or N2. Teragram quantities of N2O are emitted annually from agricultural soils treated with nitrogenous fertilizers due to the activity of soil microbiota. Although bacteria and fungi harbor genes permitting denitrification, fungi lack NosZ, an enzyme responsible for reducing N2O into inert N2 gas. Historically, scientists have linked fungi …
Managing Exoelectrogenic Microbial Community Development Through Bioprocess Control For Conversion Of Biomass-Derived Streams, Alex James Lewis
Managing Exoelectrogenic Microbial Community Development Through Bioprocess Control For Conversion Of Biomass-Derived Streams, Alex James Lewis
Doctoral Dissertations
Bioelectrochemical systems are an emerging technology capable of utilizing aqueous waste streams generated during biomass conversion of lignocellulosic feedstocks to produce valuable co-products and thus, have potential to be integrated into biorefineries. In a microbial electrolysis cell, organic compounds are converted to electrons, protons, and CO2 by fermentative and exoelectrogenic bacteria in the anode compartment. By having the ability to extract electrons from waste streams, these systems can treat water while also producing hydrogen, and thus can improve the efficiency of biomass to fuel production by minimizing external hydrogen requirement and enabling water recycle. The overall goal of this …
Evidence For The Priming Effect In Single Strain And Simplified Communities Of Estuarine Bacteria, Abigail Amina Edwards
Evidence For The Priming Effect In Single Strain And Simplified Communities Of Estuarine Bacteria, Abigail Amina Edwards
Chancellor’s Honors Program Projects
No abstract provided.
Ecological And Evolutionary Dynamics Of Plant-Soil Feedbacks: Influences On Evolution And Range Dynamics, Michael E. Van Nuland
Ecological And Evolutionary Dynamics Of Plant-Soil Feedbacks: Influences On Evolution And Range Dynamics, Michael E. Van Nuland
Doctoral Dissertations
Plants interact with, modify, and are affected by their soil environments. Though plant-soil interactions are well known to be important and active regulators of ecosystem function and community structure, much less is known about how these interactions affect plant evolution. The primary goal of my dissertation was to examine plant-soil interactions under a range of ecological and evolutionary contexts to better understand patterns of biodiversity, ecosystem function, and whole system responses to environmental change. Taking such an eco-evolutionary perspective allows for a holistic understanding of the causes and consequences of complex abiotic and biotic interactions that link ecosystem ecology and …
The Microbial Ecology Of Bacterial Lignocellulosic Degradation In The Ocean, Hannah Laing Yee Woo
The Microbial Ecology Of Bacterial Lignocellulosic Degradation In The Ocean, Hannah Laing Yee Woo
Doctoral Dissertations
The overarching theme of my dissertation is to study the role of bacteria in lignocellulose degradation. In recent years, more research has investigated the biodegradability of lignocellulose for biofuel production. The components of the lignocellulosic plant cell wall are considered intrinsically recalcitrant due to their structure. However, we hypothesize that these components are not intrinsically recalcitrant but their biodegradation is contingent on the environmental conditions, particularly the bacterial diversity. We believe bacteria will become especially important in lignocellulose degradation in conditions that are unfavorable for white-rot fungi. Therefore, we investigated the potential for lignin degradation by bacteria in the ocean …
Heat Inactivation Kinetics Of Tulane Virus And Aichi Virus, Sukriti Ailavadi
Heat Inactivation Kinetics Of Tulane Virus And Aichi Virus, Sukriti Ailavadi
Masters Theses
Human noroviruses (HNoVs) and Aichi virus (AiV) causes significant number of gastrointestinal diseases worldwide. Tulane virus (TV), a cultivable HNoV surrogate, is used to determine control measures against HNoV. The objectives of this study were to determine the heat inactivation kinetics of TV and AiV in cell-culture media and TV in spinach using the first-order and Weibull models. TV and AiV in cell-culture media at ~7 log PFU/ml in 2-ml glass vials were heated at 50-58°C [degree Celsius] up to 10 min in a circulating water-bath. Surviving infectious viruses were enumerated by standard plaque assays using confluent host cells in …
Characterization Of The Chea2 Chemotaxis Operon In Azospirillum Brasilense, Erin Lutz
Characterization Of The Chea2 Chemotaxis Operon In Azospirillum Brasilense, Erin Lutz
Chancellor’s Honors Program Projects
No abstract provided.
A Bug’S Life: Integration Of Anaerobic Digestion And Bioelectrochemical Systems For Enhanced Energy Recovery From Wastewater Solids And Other Waste Substrates, Jeff Ryan Beegle
A Bug’S Life: Integration Of Anaerobic Digestion And Bioelectrochemical Systems For Enhanced Energy Recovery From Wastewater Solids And Other Waste Substrates, Jeff Ryan Beegle
Masters Theses
Organic waste streams, like domestic wastewater and municipal solid waste, have the potential to be used as feedstocks for biotechnology processes to produce high value products and energy. This thesis investigated the technological, economical, and environmental potential for integrated anaerobic digestion (AD) and bioelectrochemical system (BES) platforms as they were theoretically and physically evaluated for energy recovery from domestic wastewater. The first chapter of this thesis compared the theoretical energy efficiencies of converting waste directly into electricity, using AD and BES alone and in various combinations. This chapter reviewed the experimentally demonstrated energy efficiencies reported in the literature with comparisons …