Microbiology Commons^™

Systemic And Mucosal Infection Program Protective Memory Cd8 T Cells In The Vaginal Mucosa., Thandi M. Onami, P. K. Suvas, H. M. Dech, J. Zeng

Microbiology Publications and Other Works

Whether mucosal immunization is required for optimal protective CD8 T cell memory at mucosal surfaces is controversial. In this study, using an adoptive transfer system, we compare the efficacy of two routes of acute lymphocytic choriomeningitis viral infection on the generation, maintenance, and localization of Ag-specific CD8 T cells in tissues, including the vaginal mucosa. Surprisingly, at day 8, i.p. infection results in higher numbers of Ag-specific CD8 T cells in the vaginal mucosa and iliac lymph node, as well as 2-3x more Ag-specific CD8 T cells that coexpress both IFN-gamma and TNF-alpha in comparison to the intranasal route of …

Construction Of Bacteriophage-Based Bioluminescent Bioreporters For Staphylococcus Aureus And Salmonella Monitoring, Aysu Ozen

Doctoral Dissertations

Construction of two recombinant luxI bacteriophage-based bioluminescent bioreporters was undertaken to develop detection and monitoring systems for Staphylococcus aureus and Salmonella spp. These systems take advantage of the high specificity of bacteriophage for their hosts and the Vibrio fischeri lux operon responsible for quorum sensing bioluminescence. The detection system is composed of two elements, a recombinant phage with luxI which is specific for the target pathogen, and an acylhomoserine lactone (AHL)-inducible bioreporter cell line carrying the reporter lux genes. The goal of this study was to construct Salmonella- and S. aureus-specific recombinant phages which contain luxI.

The …

The Role Of Soluble Fibrin In Lymphocyte And Lak Cell Adherence To And Migration Across Vascular Endothelial Cells: Implications For Immunotherapy And Cancer, Brandy Lee Weidow

Masters Theses

Although conventional therapies for metastatic cancers have made significant progress in recent years, they are relatively nonspecific and have many deleterious side-effects. Recently, novel therapies, including adoptive cellular immune therapies have had sporadic, but spectacular success in cancers such as malignant melanoma and renal cell carcinoma: tumors in which an immune response has been demonstrated. However, other physiological mechanisms, such as blood coagulation inhibit the immune response against cancers. Our previous work has shown that one of these coagulation proteins, soluble fibrin (sFn), inhibits unstimulated and activated lymphocyte adherence to tumor cells by blocking leukocyte integrin (CD11a/CD18) binding to tumor …

Biogeography And Genetic Diversity Of Toxin Producing Cyanobacteria In A Laurentian Great Lake, Johanna Maaria Rinta-Kanto

Doctoral Dissertations

The North American Great Lakes are a vital source on a global scale, as they hold ~18 % of the potable water resources on our planet. Cyanobacteria of the genus Microcystis are commonly found in fresh water environments around the world, and since the mid-1990s also in Lake Erie. The reasons for the success for these potentially toxic cyanobacteria in Lake Erie are not completely understood. In this study we have applied modern molecular tools to analyze field samples to provide an insight into the genotypic composition and diversity of the Microcystis community in the past and present day Lake …

Scanning Probe Microscopy: Applications For Genomic Research, David Post Allison

Doctoral Dissertations

The inauguration of the International Human Genome Initiative in the later part of the 1980’s coincided with the development of scanning probe microscopy (SPM). SPM was a good fit as one of the new technologies that might be implemented to sequence or map DNA and perhaps make a major contribution toward the goal of sequencing the entire human genome. Although the scanning tunneling microscope (STM) was invented in 1982 [Binnig 1982] and the atomic force microscope (AFM) in 1986 [Binnig 1986], it was not until 1987 that the first STM became commercially available; the AFM became available in 1989.

Our …

The Microscope (2007-2008), Department Of Microbiology

The Microscope

No abstract provided.