Microbiology Commons^™

Early Events Of Lethal Action By Tobramycin In Pseudomonas Aeruginosa, Jane Elizabeth Raulston

Doctoral Dissertations

The immediate activities of the aminoglycoside antibiotic, tobramycin, were investigated in Pseudomonas aeruginosa PAO1. The influence of carbon growth substrate and the antibiotic exposure environment in the magnitude of activity were examined. Lethality by 8 ug/ml tobramycin occurred rapidly (1 to 3 minutes). The release of specific cellular component into the supernatant was associated with lethality. This material was initially detected as an increase in UV-absorbance. Magnesium in the reaction mixture provided protection against lethality and leakage, but did not reverse lethal damage after a 3 minute tobramycin treatment. Also, uptake of ³H-tobramycin was reduced in the presence of …