Microbiology Commons^™

Adaptation To Growth At Low Ph By Clostridium Sporogenes, Claudia Dee Crosthwait

Masters Theses

The ability of Clostridium sprogenes to adapt to growth at low pH was studied in tomato serum prepared by enzymatic hydrolysis of canned tomatoes and tomato juice. The lowest pH at whicn C. sporogenes could grow before adaptation in tomato serum, Trypticase Peptone Glucose Yeast Extract (TPGY) and fluid thioglycollate broths was pH 5.4.

Tomato serum and TPGY or fluid thioglycollate broth adjusted to varying pH levels were inoculated with C. sporogenes from tubes with growth at the lower pH values. Subsequent transfers of C. sporogenes grown in tomato serum were made to tomato serum of equal and lower pH …