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Intrerconversion Of Replicative Forms Of Sindbis Virus Rna And The Electrophoresis Of Viral Rna, Jonathan D. Martin

Doctoral Dissertations

Three species of double-strand (ds) RNA, designated RF I, RF II, and RF III in order of decreasing size, were produced by RNase treatment of extracts of chicken embryo cells infected for 6 h with Sindbis virus. These species were identifiable with those reported by Simmons and Strauss (28). At a low level of enzyme (0.001 µg/ml) the major species obtained was RF I. When the enzyme concentration was increased 10-, 100, and 1000-fold, there was a progressive increase in the proportions of RF's II and III and a concurrent decrease in the proportion of RF I. Only RF I …