Microbiology Commons^™

Seroprevalence And Genetic Characterization Of Toxoplasma Gondii In Three Species Of Pet Birds In China, Wei Cong, Meng Qing-Feng, Hui-Qun Song, Dong-Hui Zhou, Si-Yang Huang, Ai-Dong Qian, Chunlei Su, Xing-Quan Zhu

Microbiology Publications and Other Works

Background

Toxoplasmosis, caused by the protozoan parasite Toxoplasma gondii, is one of the most common zoonosis worldwide, affecting a wide range of warm-blooded mammals and birds worldwide. However, no information on T. gondii infection in pet birds in China is available. Therefore, this study was performed to determine the prevalence of T. gondii infection in pet birds in Gansu province, China.

Methods

A total of 687 blood samples were collected from pet birds (Carduelis spinus, Alauda gulgula, Cocothraustes migratorlus) in three representative administrative regions in Gansu province, northwest China between August 2011 and September 2012 T. gondii antibodies …

Genetic Characterization Of Toxoplasma Gondii From Qinghai Vole, Plateau Pika And Tibetan Ground-Tit On The Qinghai-Tibet Plateau, China, Xiao-Xuan Zhang, Zhong-Zi Lou, Si-Yang Huang, Dong-Hui Zhou, Wan-Zhong Jia, Chunlei Su, Xing-Quan Zhu

Microbiology Publications and Other Works

Background

The distribution of genetic diversity of Toxoplasma gondii in wildlife is of interest to understand the transmission of this parasite in the environment. Limited information on T. gondii genotypes has been reported in wildlife in China. The objective of this study was to carry out the genetic characterization of T. gondii isolates from wild animals on the Qinghai-Tibet Plateau.

Methods

Using PCR and multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology, we detected genetic diversity of T. gondii isolates from Qinghai vole, Plateau pika and Tibetan ground-tit in these regions.

Results

In total, 183 brain tissues of different …

Genetic Characterization Of Toxoplasma Gondii From Pigs From Different Localities In China By Pcr-Rflp, Hai-Hai Jiang, Si-Yang Huang, Dong-Hui Zhou, Xiao-Xuan Zhang, Chunlei Su, Shun-Zhou Deng, Xing-Quan Zhu

Microbiology Publications and Other Works

Background

Toxoplasma gondii is a widely prevalent protozoan parasite that causes serious toxoplasmosis in humans and animals. The present study aimed to determine the genetic diversity of T. gondii isolates from pigs in Jiangxi, Sichuan, Guangdong Provinces and Chongqing Municipality in China using multilocous polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology.

Methods

A total of 38 DNA samples were extracted from hilar lymph nodes of pigs with suspected toxoplasmosis, and were detected for the presence of T. gondii by semi-nested PCR of B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci, namely, …

Changes In The Proteomic Profiles Of Mouse Brain After Infection With Cyst-Forming Toxoplasma Gondii, Dong-Hui Zhou, Fu-Rong Zhao, Si-Yang Huang, Min-Jun Xu, Hui-Qun Song, Chunlei Su, Xing-Quan Zhu

Microbiology Publications and Other Works

Background

Toxoplasma gondii is an opportunistic pathogenic protozoan parasite, which infects approximately one third of the human population worldwide, causing opportunistic zoonotic toxoplasmosis. The predilection of T. gondii for the central nervous system (CNS) causes behavioral disorders and fatal necrotizing encephalitis and thus constitutes a major threat especially to AIDS patients.

Methods

In the present study, we explored the proteomic profiles of brain tissues of the specific pathogen-free (SPF) Kunming mice at 7 d, 14 d and 21 d after infection with cysts of the Toxoplasma gondii Prugniaud (PRU) strain (Genotype II), by two-dimensional gel electrophoresis (2-DE) combined with MALDI-TOF/TOF …