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Microbiology Commons

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Full-Text Articles in Microbiology

Predicting The Next Pandemic: Utilizing Wastewater-Based Epidemiology As A Novel Technique For Detecting Mpox Dna, Austin Tang, Michael Moshi, Hayley Baker, Ching-Lan Chang Dec 2022

Predicting The Next Pandemic: Utilizing Wastewater-Based Epidemiology As A Novel Technique For Detecting Mpox Dna, Austin Tang, Michael Moshi, Hayley Baker, Ching-Lan Chang

Undergraduate Research Symposium Lightning Talks

Mpox cases are trending downward in Las Vegas, aligning with national trends. The study demonstrates that it is possible to extract mpox DNA directly from wastewater and quantify the genetic material using qPCR. This research indicates that WBE can be utilized to give a relative abundance of virual nucleic acids in wastewater, giving opportunities for early-warning systems and other public health interventions. Mpox remains a global threat to public health, continued surveillance of MPXV using wastewater is necessary to ensure that we can detect unusual spike in viral DNA level. Mpox detected in the wastewater samples will be sequences to …


Quantification Of Sars-Cov-2 Prevalence Using Wastewater Surveillance, Moonis Ghani, Hayley Baker, Nabih Ghani, Anthony Harrington, Ching-Lan Chang Dec 2022

Quantification Of Sars-Cov-2 Prevalence Using Wastewater Surveillance, Moonis Ghani, Hayley Baker, Nabih Ghani, Anthony Harrington, Ching-Lan Chang

Undergraduate Research Symposium Lightning Talks

SARS-CoV-2 RNA was successfully detected in the wastewater samples. PMMoV concentrations varied little, meaning it was a valid control. The diversity of SARS-CoV-2 concentrations indicated varying levels of infection within different parts of the community. PCR and WGS can help determine viral load and identify variants.


Calcite Spherulite Formations And Their Controls In Halophilic Bacterial Cultures, Zakaria Jibrin Dec 2022

Calcite Spherulite Formations And Their Controls In Halophilic Bacterial Cultures, Zakaria Jibrin

UNLV Theses, Dissertations, Professional Papers, and Capstones

This study arose from an accidental discovery of calcite spherulites forming on colonies of various halophilic bacteria grown on solid Luria-Bertani medium made containing water from a brine pool in Death Valley, their natural habitat. Further studies with a large spherulite forming bacteria, Pseudoalteromonas haloplanktis, showed that calcite precipitation conditions were the consequence of bacteria using amino acids as a source of carbon and energy, a metabolism that produces NH3 and CO2 waste products. NH3 raises the pH of the medium, turning CO2 into CO32-. Calcium in the natural brine combines with CO32-. The resulting spherulitic calcite precipitation is nano-acicular …


Microbial Contamination On Ambulance Surfaces: A Systematic Literature Review, Adam Obenza, Patricia Cruz, Mark Buttner, D. Woodard Apr 2022

Microbial Contamination On Ambulance Surfaces: A Systematic Literature Review, Adam Obenza, Patricia Cruz, Mark Buttner, D. Woodard

Environmental & Occupational Health Faculty Publications

Healthcare-associated infections (HAIs) are infections that patients acquire while receiving medical treatment in a healthcare facility. During ambulatory transport, the patient may be exposed to pathogens transmitted from emergency medical service (EMS) personnel or EMS surfaces. The aim of this study was to determine whether organisms commonly associated with HAIs have been detected on surfaces in the patient-care compartment of ambulances. Five electronic databases – PubMed, Scopus, Web of Science, Embase and Google Scholar were used to search for articles using inclusion and exclusion criteria following the PRISMA checklist. Inclusion criteria consisted of articles published in English, between 2009 and …