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Full-Text Articles in Microbiology
Genomic Organization Of Infectious Salmon Anemia Virus, Trent Rector
Genomic Organization Of Infectious Salmon Anemia Virus, Trent Rector
Electronic Theses and Dissertations
Infectious salmon anemia virus (ISAV) is an emerging pathogen of farmed Atlantic salmon (Salmo salar). The development of an effective ISA virus vaccine is a high priority for salmon producers in the U.S. and elsewhere. The process of developing a recombinant vaccine requires complete genetic characterization of the virus. Toward this end we have cloned, sequenced and determined the organization of the eight segments of single-stranded RNA from ISA virus isolate CCBB. The virus was grown in cell culture and purified by density gradient ultracentrifugation. Viral RNA was isolated from purified ISAV and used in the construction of two different …
Polycyclic Aromatic Hydrocarbon Degradation By Novel Bacteria Isolated From Burrow Sediments Of Marine Benthic Macrofauna, Wai Ki Chung
Polycyclic Aromatic Hydrocarbon Degradation By Novel Bacteria Isolated From Burrow Sediments Of Marine Benthic Macrofauna, Wai Ki Chung
Electronic Theses and Dissertations
Polycyclic aromatic hydrocarbons (PAH) are significant environmental pollutants and represent a severe health threat. Many cosmopolitan areas including coastal environments are heavily impacted by PAH. This research investigates the isolation of PAH-degrading bacteria from burrow sediment of marine benthic macrofauna and their potential in bioremediation. Macrofaunal burrow sediment is biogeochemically distinct from bulk sediment and has high microbial activities. Samples were collected from a local uncontaminated cove and PAH degradation potential was measured by incubating sediment slurries with exogenous PAH. Burrow sediments from two polychaetes and a mollusc showed significantly higher PAH degradation potential than the bulk sediment. The degradation …