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Full-Text Articles in Microbiology
Hsv-1 Replication In Different Raw 264.7 And J774.1 Macrophage Phenotypes And Macrophage Viability Following Hsv-1 Infection, Yousef Nifaj Alanazi
Hsv-1 Replication In Different Raw 264.7 And J774.1 Macrophage Phenotypes And Macrophage Viability Following Hsv-1 Infection, Yousef Nifaj Alanazi
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HSV-1 is a ubiquitous virus capable of causing lifelong latent infection. The virus contains a large double strand DNA genome covered by an icosahedral capsid. HSV-1 is a cytolytic virus which can cause a lethal infection. The virus possesses critical protein ICP0 which is capable of interfering with host cell signaling and eventually prevent cell apoptosis. Innate immunity plays a crucial role in defense against HSV-1 infection and macrophage plays a significant role in the innate immune system. Macrophage cells can alter their behavior depending upon certain stimuli and tissue environments. Naive macrophage (M0) cells can be polarized to a …
Virus Production And Cell Viability Of Hsv-1-Infected Murine Keratinocytes (Hel-30) Co-Cultured With Murine Macrophages (Raw 264.7), Barry Graffagna
Virus Production And Cell Viability Of Hsv-1-Infected Murine Keratinocytes (Hel-30) Co-Cultured With Murine Macrophages (Raw 264.7), Barry Graffagna
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Keratinocytes are the most abundant type of cell in the outer layer of skin, the epidermis, and provide barrier against pathogens from invading. However, Herpes Simplex Virus Type 1 (HSV-1) targets these keratinocytes for infection, and later infects neurons to establish lifelong latency. The keratinocytes stimulate the innate immune system to engage and to destroy the virus. Among the cells of the innate immune system to respond to the viral invasion is the macrophage. In this study, RAW 264.7 macrophage and HEL-30 keratinocyte monolayers were challenged in vitro with HSV-1 at a multiplicity of infection (MOI) of 0.1 to investigate …