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Full-Text Articles in Microbiology
Downregulation Of Microrna Eca-Mir-128 In Seminal Exosomes And Enhanced Expression Of Cxcl16 In The Stallion Reproductive Tract Are Associated With Long-Term Persistence Of Equine Arteritis Virus, Mariano Carossino, Pouya Dini, Theodore S. Kalbfleisch, Alan T. Loynachan, Igor F. Canisso, Kathleen M. Shuck, Peter J. Timoney, R. Frank Cook, Udeni B. R. Balasuriya
Downregulation Of Microrna Eca-Mir-128 In Seminal Exosomes And Enhanced Expression Of Cxcl16 In The Stallion Reproductive Tract Are Associated With Long-Term Persistence Of Equine Arteritis Virus, Mariano Carossino, Pouya Dini, Theodore S. Kalbfleisch, Alan T. Loynachan, Igor F. Canisso, Kathleen M. Shuck, Peter J. Timoney, R. Frank Cook, Udeni B. R. Balasuriya
Maxwell H. Gluck Equine Research Center Faculty Publications
Equine arteritis virus (EAV) can establish long-term persistent infection in the reproductive tract of stallions and is shed in the semen. Previous studies showed that long-term persistence is associated with a specific allele of the CXCL16 gene (CXCL16S) and that persistent infection is maintained despite the presence of a local inflammatory and humoral and mucosal antibody responses. In this study, we demonstrated that equine seminal exosomes (SEs) are enriched in a small subset of microRNAs (miRNAs). Most importantly, we demonstrated that long-term EAV persistence is associated with the downregulation of an SE-associated miRNA (eca-mir-128) and with an enhanced …
Evolution Of Equine Arteritis Virus During Persistent Infection In The Reproductive Tract Of The Stallion And The Male Donkey, Bora Nam
Theses and Dissertations--Veterinary Science
Equine arteritis virus (EAV) establishes persistent infection in the stallion reproductive tract, and the carrier stallion continues to shed virus in semen for weeks to years or lifelong. The objective of this study was to elucidate the intra-host evolution of EAV during persistent infection in stallions. Seven EAV seronegative stallions were experimentally infected with EAV KY84 strain and followed for 726 days post-infection, and sequential clinical samples including semen were collected for virus isolation and next-generation sequencing (NGS). In addition, archived sequential semen samples from two stallions that were naturally infected with EAV KY84 for a long-period (up to 10 …