Open Access. Powered by Scholars. Published by Universities.®

Microbiology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 4 of 4

Full-Text Articles in Microbiology

16s Rrna Gene Sequence And Phylogenetic Tree Of Lactobacillus Species From The Vagina Of Healthy Nigerian Women, Kingsley Anukam, Emmanuel Osazuwa, Ijeoma Ahonkhai, Gregor Reid Oct 2005

16s Rrna Gene Sequence And Phylogenetic Tree Of Lactobacillus Species From The Vagina Of Healthy Nigerian Women, Kingsley Anukam, Emmanuel Osazuwa, Ijeoma Ahonkhai, Gregor Reid

Kingsley C Anukam

Lactobacilli are ubiquitous in nature and in humans they play a very significant role in the general health maintenance of the host. Identification of Lactobacilli has previously been based on cultutre-dependent methods and recently molecular techniques involving gene sequencing are now the ‘gold standard’. Scarce information exists in Africa on the real identity of Lactobacillus species, albeit phylogenetic distances among the species present in the human vagina. 185 vaginal swabs were collected from healthy premenopausal women (18-48 years). Bacterial DNA was extracted, amplified using PCR, with group specific Lactobacillus primers, and processed by denaturing gradient gel electrophoresis (DGGE). DGGE bands …


Two Forms Of The Large Tumor Suppressor Gene (Lats1) Protein Expressed In The Vertebrate Retina, Novrouz B. Akhmedov, Clyde K. Yamashita, Dai Tran, Natik I. Piri, Gustavo D. Aguirre, Debora B. Farber Mar 2005

Two Forms Of The Large Tumor Suppressor Gene (Lats1) Protein Expressed In The Vertebrate Retina, Novrouz B. Akhmedov, Clyde K. Yamashita, Dai Tran, Natik I. Piri, Gustavo D. Aguirre, Debora B. Farber

Gustavo D. Aguirre, VMD, PhD

The large tumor suppressor gene (Lats1) encodes a protein kinase that is highly conserved from fly to human, and plays a crucial role in the prevention of tumor formation by controlling mitosis progression. We have found that in addition to the previously isolated 7.5 kb long form of Lats1 (Lats1L) mRNA, a less abundant, shorter, 3.4 kb primary transcript (Lats1S) also is expressed in the vertebrate retina. Compared toLats1L, the sequence of Lats1S mRNA has a deletion of exons 6, 7, and 8 that corresponds to 792 bp …


What Communities Should Do Pre-Event To Support Public Health Post-Event Assessments, Surveillance And Monitoring, Thomas Lyons Carr Iii Feb 2005

What Communities Should Do Pre-Event To Support Public Health Post-Event Assessments, Surveillance And Monitoring, Thomas Lyons Carr Iii

Thomas Lyons (Thom) Carr III Appl.Sc., CEM

[Abstract written March 2008, TLC] Under worst-case planning assumptions used by some major metropolitan areas, a Neighbor-to-Neighbor self-help program model is the primary link between citizens and the professional response personnel of the responsible government agencies.

In the Neighbor-to-Neighbor self-help program model or a Community Emergency Management Plan (CEMP) calls on the citizens in neighborhoods to identify and establish cluster emergency preparedness committees, Cluster Emergency Coordination Centers (CECC) and Community Emergency Response Teams (CERT). Missing from these plans or what is not articulated is how constant Public Health Post-Event Surveillance, Monitoring and Assessments will be done. Given the worst-case planning …


Lack Of Antigen-Specific Tissue Remodeling In Mice Deficient In The Macrophage Galactose-Type Calcium-Type Lectin 1/Cd301a., Thandi M. Onami, K. Sato, Y. Imai, N. Higashi, Y. Kumamoto, S. M. Hedrick, T. Irimura Jan 2005

Lack Of Antigen-Specific Tissue Remodeling In Mice Deficient In The Macrophage Galactose-Type Calcium-Type Lectin 1/Cd301a., Thandi M. Onami, K. Sato, Y. Imai, N. Higashi, Y. Kumamoto, S. M. Hedrick, T. Irimura

Thandi M. Onami

Macrophage galactose-type C-type lectins (MGLs), which were recently named CD301, have 2 homologues in mice: MGL1 and MGL2. MGLs are expressed on macrophages and immature dendritic cells. The persistent presence of granulation tissue induced by a protein antigen was observed in wild-type mice but not in mice lacking an endogenous, macrophage-specific, galactose-type calcium-type lectin 1 (MGL1) in an air pouch model. The anti-MGL1 antibody suppressed the granulation tissue formation in wild-type mice. A large number of cells, present only in the pouch of MGL1-deficient mice, were not myeloid or lymphoid lineage cells and the number significantly declined after administration of …