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Microbiology Commons

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Biology

University of Tennessee, Knoxville

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Full-Text Articles in Microbiology

The Full Genome Sequence Of An Antarctic Microbe Constructed Using A Rapid, Portable Sequencer And A Hybrid Assembly, Bruce Wyatt Boles Apr 2019

The Full Genome Sequence Of An Antarctic Microbe Constructed Using A Rapid, Portable Sequencer And A Hybrid Assembly, Bruce Wyatt Boles

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Microbial genomes contain combinations of nucleotides that code for genes which subsequently determines the function of the cell. This information provides significant insight into the unique niche of microbes from extreme environments. Here we use two sequencing technologies, the Oxford Nanopore MinION with accompanying software and Illumina HiSeq, to generate a hybrid genome for a Shewanella strain isolated from an Antarctic glacier. Bioinformatic programs, Albacore and SPAdes, allowed us to decrease the time of genome assembly while also obtaining a large quantity of information related to the Shewanella sp. Our combined approach yielded a high quality genome assembly 5.3 …


Synechococcus As An Hooh-Consuming Helper For Prochlorococcus, Abigail Mcgettigan Jarratt May 2018

Synechococcus As An Hooh-Consuming Helper For Prochlorococcus, Abigail Mcgettigan Jarratt

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

The marine cyanobacterium Prochlorococcus is an abundant and globally important microbe that contributes an estimated 40% of bacterial production in the oligotrophic ocean. The success of this photosynthetic bacterium is largely a consequence of its small cell size and streamlined genome, which are advantageous in nutrient-limited environments. However, this genomic streamlining has also led to the loss of the gene (katG) encoding catalase, an enzyme that is essential for the degradation and detoxification of the reactive oxygen species hydrogen peroxide (HOOH). HOOH is naturally present in the illuminated waters of the ocean, and in the absence of catalase, Prochlorococcus is …


Quantitative Depth Profile Of Prochlorococcus In The Pacific Ocean, Benjamin Carter Calfee Apr 2014

Quantitative Depth Profile Of Prochlorococcus In The Pacific Ocean, Benjamin Carter Calfee

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Prochlorococcus is a genus of extremely abundant marine cyanobacterium. This microbe is responsible for the majority of the primary production within marine environments and is thought to be the single most abundant photosynthetic organism. In addition to accounting for such a large portion of the world’s photosynthetic activity, Prochlorococcus functions with a genome much smaller than most other primary producers. Thus due to its general abundance and overall importance in oceanic ecosystems, an experiment was prompted to derive the spatial and numerical separation of the members of this genus across the Pacific Ocean through the use of quantitative polymerase chain …


Comparison Of Bacterial Communities Associated Spider Species Occupying Different Physical Environments, Jack Kang Apr 2014

Comparison Of Bacterial Communities Associated Spider Species Occupying Different Physical Environments, Jack Kang

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

No abstract provided.