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1979

VIMS Articles

Microbiology

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Full-Text Articles in Marine Biology

Sublethal Stress In Escherichia-Coli - Function Of Salinity, I. C. Anderson, Mw Rhodes, H Kator Dec 1979

Sublethal Stress In Escherichia-Coli - Function Of Salinity, I. C. Anderson, Mw Rhodes, H Kator

VIMS Articles

Sublethal stress in Escherichia coli was detected in various test media after exposure (in vitro) to seawater of various salinities. Stress was measured with an electrochemical detection technique and a,-galactosidase assay. Test media included EC medium, medium A-1, and tryptic soy broth modified to contain lactose for /?-galactosidase assay experiments. Stress was defined as the difference between a predicted electrochemical response time calculated for unstarved cells from a standard curve and the observed electrochemical response time for cells starved in seawater. The higher the salinity, the greater the stress for all test media examined. Stress was most pronounced in EC …