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Full-Text Articles in Marine Biology

Application Of The Analytic Hierarchy Process And Geographic Information System In Selecting Sites For A Seafood Market Study, Shielameh Peralta-Milan, Osamu Baba, Severino Iii Salmo Mar 2024

Application Of The Analytic Hierarchy Process And Geographic Information System In Selecting Sites For A Seafood Market Study, Shielameh Peralta-Milan, Osamu Baba, Severino Iii Salmo

The Philippine Agricultural Scientist

Study site selection is essential for the success of many fisheries-related programs. A thorough site selection could provide transparency in the decision-making process and optimize resources. In this research, the analytic hierarchy process (AHP) approach was used to select sites for a market study of reef-associated seafood in Alaminos City, Northwestern Philippines. Out of the 10 coastal barangays, four (Lucap, Sabangan, Telbang, and Victoria) were initially chosen, and the difference between these sites and the priority sites generated from AHP was examined. Five decision criteria and 10 alternatives (coastal barangays) were analyzed to calculate priority weights using pairwise comparisons. The …


Protective Responses Of The Intestinal Epithelial Cell Line Ht-29 Cells Exposed To Dephosphorylated Salmonella Flagellin [Research Note], Heyeon Baik, Jaiesoon Cho Dec 2023

Protective Responses Of The Intestinal Epithelial Cell Line Ht-29 Cells Exposed To Dephosphorylated Salmonella Flagellin [Research Note], Heyeon Baik, Jaiesoon Cho

The Philippine Agricultural Scientist

This study aimed to describe the effects of Salmonella Typhimurium flagellin (SFL) dephosphorylated by sweet potato purple acid phosphatase (PAP) on the protective responses of the intestinal epithelial cell line HT-29 cells. The enzyme was reported to display a broad substrate specificity for various organic phosphorylated conjugates and phosphoproteins. Dephosphorylation of SFL by sweet potato PAP decreased to 35% in the presence of 0.05 mM vanadate as compared with the negative control (p < 0.05). Intact SFL and the SFL treated with sweet potato PAP did not remarkably induce the activation of caspase-3 in HT-29 cells at all the tested levels of the substrate. Intact SFL maximally induced the release of IL (interleukin)-8 in HT-29 cells at 1000 ng/mL (p < 0.05). However, the SFL treated with the enzyme inhibited the release of IL-8 at over 100 ng/mL of the substrate as compared with intact SFL, resulting in an approximately 8-fold decrease even at 1000 ng/mL (p < 0.05). The SFL treated with the enzyme decreased the activation of the total ERK1/2 in the cells to 1.9 and 1.7–fold at 10 and 1000 ng/mL of the substrate, respectively, as compared with intact SFL (p < 0.05). In conclusion, sweet potato PAP could be a promising tool for controlling excessive inflammation during Salmonella infection in animal husbandry, and the enzyme could be a safe alternative that can overcome the drawbacks of chemotherapy.


Alternate Day Feeding As A Cost-Effective Strategy For Tank Culture Of The Pacific Shortfin Eel Anguilla Bicolor Pacifica, Frolan A. Aya, John Carlo L. Unida, Maria Rowena R. Romana-Eguia, Nerissa D. Salayo Sep 2023

Alternate Day Feeding As A Cost-Effective Strategy For Tank Culture Of The Pacific Shortfin Eel Anguilla Bicolor Pacifica, Frolan A. Aya, John Carlo L. Unida, Maria Rowena R. Romana-Eguia, Nerissa D. Salayo

The Philippine Agricultural Scientist

There is a growing interest in the aquaculture of tropical anguillid eels as an export commodity. However, studies on feeding strategies, and the present demand to reduce feed costs need to be addressed to ensure the economic viability of eel farming. In this study, the effects of daily (DF) and alternate day (ADF) feeding on growth, feed utilization, body composition, blood chemistry, liver and intestinal morphology, and economic viability in the Pacific shortfin eel Anguilla bicolor pacifica were examined. Each feeding group of 30 elvers (166.25 ± 16.23 g mean initial wt) were randomly stocked in triplicate 4 m3 …


Reproduction, Early Development, And Larval Rearing Of The Climbing Perch Anabas Testudineus (Teleostei: Anabantidae) In Captivity, Frolan A. Aya, Reylan C. Gutierrez, Luis Maria B. Garcia Jun 2023

Reproduction, Early Development, And Larval Rearing Of The Climbing Perch Anabas Testudineus (Teleostei: Anabantidae) In Captivity, Frolan A. Aya, Reylan C. Gutierrez, Luis Maria B. Garcia

The Philippine Agricultural Scientist

Induced spawning, early development, and larval rearing of the climbing perch Anabas testudineus were examined under captive conditions. Five female climbing perch [125.33 ± 3.27 mm SL; 70.67 ± 5.59 g BW] were paired with apparently mature males (108.50 ± 3.97 mm SL; 39.27 ± 4.70 g BW) and induced to spawn with 5,000 IU human chorionic gonadotropin (hCG) + 0.5 mL Ovaprim kg BW-1. All hCG + Ovaprim-injected fish spawned 9 – 11 h after hormone administration but saline-injected fish (control group) failed to spawn. Mean egg production per female, fertilization and hatching rates, and larval production …


Behavior Changes And Lc50 Of Dried Garlic (Allium Sativum) Acute Toxicity In Nile Tilapia (Oreochromis Niloticus) Juvenile [Research Note], Gregoria Erazo Pagador, Haydee Rose Dumaran Paciente, Blan Jericho Caloyloy Jun 2023

Behavior Changes And Lc50 Of Dried Garlic (Allium Sativum) Acute Toxicity In Nile Tilapia (Oreochromis Niloticus) Juvenile [Research Note], Gregoria Erazo Pagador, Haydee Rose Dumaran Paciente, Blan Jericho Caloyloy

The Philippine Agricultural Scientist

Plant-based compounds are emerging substitutes for chemical treatments in aquaculture since they are known to cause less harm to both the environment and its organisms. Garlic (Allium sativum) is a popular herb that has a broad range of uses, especially in the control and treatment of bacterial and parasitic diseases. However, its toxicity to tilapia is not well-studied. Hence, this study was conducted to test the toxicity levels of garlic powder in juvenile Nile tilapia (Oreochromis niloticus) by determining the cumulative mortality and median lethal concentration (LC50). Test fish were exposed through immersion in …


Grow-Out Culture Of Oyster Magallana Bilineata (Röding, 1798) Using Pouches: A Comparison Of Growth And Survival In The River And Earthen Pond, Ma. Junemie Hazel L. Lebata-Ramos, Ellen Flor D. Solis, Mark Jude C. Almeida Mar 2023

Grow-Out Culture Of Oyster Magallana Bilineata (Röding, 1798) Using Pouches: A Comparison Of Growth And Survival In The River And Earthen Pond, Ma. Junemie Hazel L. Lebata-Ramos, Ellen Flor D. Solis, Mark Jude C. Almeida

The Philippine Agricultural Scientist

Oysters are among the most in-demand aquaculture commodities in the Philippines and worldwide. With the decreasing culture area and the deteriorating water quality of oyster beds, there is a need to explore new culture sites and techniques to address the problems of dwindling stocks and the increasing demand for better quality oysters. This study compared the growth and survival of the oysters Magallana bilineata, the new accepted name of Crassostrea iredalei, cultured in an earthen pond and the river using pouches suspended from rafts. Mean growth rates of oysters in length and weight were significantly higher in those …