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Journal of Marine Science and Technology

Nitrogen

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Full-Text Articles in Marine Biology

Sequence Diversity Of Ammonium Transporter Genes In Cultured And Natural Species Of Marine Phytoplankton, Lee-Kuo Kang, Jeng Chang Feb 2014

Sequence Diversity Of Ammonium Transporter Genes In Cultured And Natural Species Of Marine Phytoplankton, Lee-Kuo Kang, Jeng Chang

Journal of Marine Science and Technology

The sequence database of ammonium transporter (Amt1) genes in eukaryotic phytoplankton was expanded by obtaining new sequences from cultured strains and natural populations collected in the East China Sea (ECS). From unialgal cultures, 6 new Amt1 sequences belonging to the Bacillariophyceae, Dinophyceae, and Prasinophyceae were obtained. A phylogenetic analysis revealed that AMT1s of higher plants were most closely related to those in green algae. In addition, haptophyte and diatom AMT1s formed distinct monophyletic clades. Diatom AMT1s were further divided into 3 orthologous subclasses, and active gene duplications were observed in subclass III. As for mixed-species sequencing using ECS samples, 78 …