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Full-Text Articles in Marine Biology
The Use Of Extractable Lipofuscin For Age Determination Of Crustaceans: Reply To Sheehy (2008), H. Rodger Harvey, David H. Secor, Se-Jong Ju
The Use Of Extractable Lipofuscin For Age Determination Of Crustaceans: Reply To Sheehy (2008), H. Rodger Harvey, David H. Secor, Se-Jong Ju
OES Faculty Publications
The heterogeneous mixture of metabolic by-products termed lipofuscin (LF) or age pigments has long been known to accumulate in post mitotic cells with increasing age. In crustaceans several approaches have been developed over the years to track LF accumulation and provide a proxy for chronological age. Histological approaches have been traditionally used for LF determination of crustaceans (e.g. Sheehy et al. 1994, Mar Biol 121:237-245), but over the last decade extraction approaches for neural tissues have been introduced (Ju et al. 1999, Mar Ecol Prog Ser 185:171-179) and tested (Ju et al. 2001, Mar Ecol Prog Ser 224:197-205; 2003, Fish …
Demographic Assessment Of The Blue Crab (Callinectes Sapidus) In Chesapeake Bay Using Extractable Lipofuscins As Age Markers, Se-Jong Ju, David H. Secor, H. Rodger Harvey
Demographic Assessment Of The Blue Crab (Callinectes Sapidus) In Chesapeake Bay Using Extractable Lipofuscins As Age Markers, Se-Jong Ju, David H. Secor, H. Rodger Harvey
OES Faculty Publications
The blue crab (Callinectes sapidus) plays an important economic and ecological role in estuaries and coastal habitats from the Gulf of Mexico to the east coast of North America, but demographic assessments are limited by length-based methods. We applied an alternative aging method using biochemical measures of metabolic byproducts (lipofuscins) sequestered in the neural tissue of eyestalks to examine population age structure. From Chesapeake Bay, subsamples of animals collected from the 1998-99 (n-769) and 1999-2000 (n=367) winter dredge surveys were collected and lipofuscin was measured. Modal analysis of the lipofuscin index provided separation into three modes, whereas carapace-width …
Growth Rate Variability And Lipofuscin Accumulation Rates In The Blue Crab Callinectes Sapidus, Se-Jong Ju, David H. Secor, H. Rodger Harvey
Growth Rate Variability And Lipofuscin Accumulation Rates In The Blue Crab Callinectes Sapidus, Se-Jong Ju, David H. Secor, H. Rodger Harvey
OES Faculty Publications
To better understand growth and age-pigment (lipofuscin) accumulation rates of the blue crab Callinectes sapidus under natural conditions, juveniles (33 to 94 mm carapace width) were reared in outdoor ponds for over 1 yr. Growth rates, measured by carapace width, during summer and fall exceeded all those reported in the literature; the initial carapace width of 59 ± 14 mm (mean ± SD) increased to 164 ± 15 mm within a 3 mo period. No growth occurred during winter months (November to April) at low water temperatures. Growth rates of crabs in ponds were substantially higher (von Bertalanffy growth parameter …
Use Of Extractable Lipofuscin For Age Determination Of Blue Crab Callinectes Sapidus, Se-Jong Ju, David H. Secor, H. Rodger Harvey
Use Of Extractable Lipofuscin For Age Determination Of Blue Crab Callinectes Sapidus, Se-Jong Ju, David H. Secor, H. Rodger Harvey
OES Faculty Publications
The blue crab Callinectes sapidus is an economically and ecologically important species in many temperate estuaries, yet stock assessments have been limited to length-based methods for demographic analyses. We evaluated the potential of age pigments (lipofuscins) sequestered in neural tissue of eye-stalks and brains to estimate the age of blue crabs collected from Chesapeake Bay and Chincoteague Bay. The rate of lipofuscin accumulation was determined using crabs of known age reared in the laboratory. Age pigments were extracted from neural tissues (eye-stalk or brain), quantified, and normalized to protein content to allow comparisons across tissue types and crab sizes. Field-collected …