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Full-Text Articles in Marine Biology
Variation In The Fluorescence Of Green Fluorescent Protein In Various Strains Of Escherichia Coli, Paul R. Letellier Jr.
Variation In The Fluorescence Of Green Fluorescent Protein In Various Strains Of Escherichia Coli, Paul R. Letellier Jr.
Biological Sciences Theses & Dissertations
Green fluorescent protein (GFP) was first identified in the jellyfish Aequorea victoria and generated interest due to its ability to noninvasively tag and visualize cells, cellular structures, and cellular proteins. Furthermore, the expression of green fluorescent protein in Esherichia coli cells as a tool for reporting gene expression and promotor activity has become important in the understanding of gene regulation. Expression of the gfp gene was found to vary depending on the strain of E. coli possessing the gfp gene. As a result of this fluctuation in fluorescence, gene expression and hence promotor activity may be difficult to detect or …
Morphological And Molecular Defects In Zebrafish Embryos (Danio Rerio) After Chromated Copper Arsenate Exposure, Kerry Jean Lee
Morphological And Molecular Defects In Zebrafish Embryos (Danio Rerio) After Chromated Copper Arsenate Exposure, Kerry Jean Lee
Biological Sciences Theses & Dissertations
This investigation identified the morphological defects caused by different concentrations of chromium, copper, and arsenic (CCA) on the developing zebrafish embryo; in addition to defects caused by the three combined components (complete CCA). A change in the DNA of the developing embryos in response to the three components of CCA and complete CCA was also determined. Early Dania rerio embryos were cultured for five days with various concentrations of CCA components and complete CCA. Morphological defects were assessed with light microscopy and DNA fragmentation was determined by agarose gel electrophoresis. Embryonic exposure of D. rerio to sub lethal concentrations of …
Feeding Behavior And Conditioning In Two Heterotrophic Dinoflagellates, Todd A. Egerton
Feeding Behavior And Conditioning In Two Heterotrophic Dinoflagellates, Todd A. Egerton
Biological Sciences Theses & Dissertations
Growth and abundance of heterotrophic dinoflagellate predators are generally attributed to the availability of algal prey. Several species of dinoflagellates feed on a wide variety of food types including fish. However the actual feeding preferences of dinoflagellates have been much less studied. In the few studies that have been carried out on dinoflagellate feeding preference, none have looked at possible factors that may affect preference. I conducted three experiments on the toxic dinoflagellate Pfiesteria piscicida and the related unnamed species Cryptoperidiniopsis brodyi which, (1) calculated the feeding preferences between algal Rhodomonas prey and fish blood cells and identified factors that …