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Research Methods in Life Sciences

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Full-Text Articles in Marine Biology

Quantifying The Respiratory Plasticity Of Common Fishes Of The Indian River Lagoon, Logan Exton Jan 2024

Quantifying The Respiratory Plasticity Of Common Fishes Of The Indian River Lagoon, Logan Exton

Honors Theses

Increasing water temperatures resulting from global climate change introduce new energetic demands for marine organisms. Higher energy input will be required to cope with a subsequently higher metabolic rate, affecting all aspects of an individual’s life and therefore their survival. Because estuaries act as a link between rivers and oceans, they and their inhabitants are considered to be the most threatened by climate change. Therefore, it is crucial to understand how these organisms will respond to increased stressors due to climate change. Checkered pufferfish (Sphoeroides testudineus) are among the most common teleost fish in the Indian River Lagoon, …


Tropical Fish Study In Tahiti, French Polynesia, Miranda Brainard, Caitlyn Swango, Paityn Houglan, Richard Londraville Jan 2024

Tropical Fish Study In Tahiti, French Polynesia, Miranda Brainard, Caitlyn Swango, Paityn Houglan, Richard Londraville

Williams Honors College, Honors Research Projects

In May of 2023, I embarked on an exciting research journey to Moorea, French Polynesia, alongside fellow students and faculty members from the University of Akron and Syracuse University. This expedition was part of the university-sponsored Tropical Vertebrate Biology course, where we delved into the exploration of various tropical species inhabiting the island, including sea urchins, geckos, and my primary focus, the blackspotted rockskipper.

My research team, composed of my co-authors and me, was particularly intrigued by the unique refuge-seeking behavior displayed by blackspotted rockskippers. These amphibious fish are renowned for their remarkable ability to inhabit tide pools and rocky …


Interactions Between Sediment Mechanical Structure And Infaunal Community Structure Following Physical Disturbance, William Cyrus Roger Clemo Dec 2023

Interactions Between Sediment Mechanical Structure And Infaunal Community Structure Following Physical Disturbance, William Cyrus Roger Clemo

<strong> Theses and Dissertations </strong>

Shallow, river-influenced coastal sediments are important for global carbon storage and nutrient cycling and provide a habitat for diverse communities of invertebrates (infauna). Elevated bed shear stress from extreme storms can resuspend, transport, and deposit sediments, disrupting the cohesive structure of muds, and sorting and depositing sand eroded from beaches. These physical disruptions can also resuspend or smother infauna, decreasing abundances and changing community structure. Infaunal activities such as burrowing, tube construction, and feeding can impact sediment structure and stability. However, little is known about how physical disturbance impacts short and long-term sediment habitat suitability and whether disturbance-tolerant infauna influence …


Deep Ocean Vehicle Applications And Modifications, Nichole "Nikki" T. Arm Dec 2023

Deep Ocean Vehicle Applications And Modifications, Nichole "Nikki" T. Arm

Master's Theses

This project had two primary goals: (1) to explore opportunities to further a deep-ocean vehicle’s reach using alternative pressure spheres, and (2) to implement an existing deep-ocean vehicle (lander) in active scientific research.

I gained a greater understanding of the limitations and design choices made for existing pressure spheres using Finite Element Analysis (FEA). My simplified FEA model predicted sphere failure for the existing 30% Fiber Glass 70% Nylon injection molded spheres at an external pressure of 3,954psi or 2,690m ocean-depth (only a 7.38% error compared to the tested minimum failure depth), so I determined it a valid model. I …


Cadmium And Salinity Stressor Antagonism On Vallisneria Neotropicalis, Christopher P. Mikolaitis Jul 2023

Cadmium And Salinity Stressor Antagonism On Vallisneria Neotropicalis, Christopher P. Mikolaitis

<strong> Theses and Dissertations </strong>

Submerged macrophytes form the foundation of freshwater ecosystems. These organisms are sessile and are very susceptible to shifts in their environment. Heavy metals are of particular concern as they can be sequestered indefinitely in sediments and are readily taken up by rooted vegetation. In the presence of saltwater intrusions, these metals can interact with salt ions potentially changing their availability to submerged vegetation. In this study a freshwater macrophyte, Vallisneria neotropicalis, was used as a test species for interactive effects between Cd, a non-essential heavy metal, and salt stress. The metrics used to establish the individual as well as …


Fecal Pellet Production By North Atlantic Zooplankton, Michael Gibson May 2023

Fecal Pellet Production By North Atlantic Zooplankton, Michael Gibson

Undergraduate Honors Theses

Fecal pellet carbon (FPC) production by zooplankton is a significant component of the ocean’s biological carbon pump: the suite of biological processes that mediate export of carbon to the deep ocean, ultimately leading to the sequestration of atmospheric carbon dioxide in the ocean. In this study, mesozooplankton (zooplankton 0.2 mm to ~2 cm) were collected from the epipelagic zone in the temperate North Atlantic Ocean during day and night in May 2021. Zooplankton were live separated into five size fractions and incubated on board ship in natural surface seawater to measure fecal pellet production rate of the mixed mesozooplankton community. …


Movement, Behavior, And Trophic Ecology Of A Pelagic Predator Guild In The Eastern Tropical Pacific Ocean, Ryan Keith Logan Mar 2023

Movement, Behavior, And Trophic Ecology Of A Pelagic Predator Guild In The Eastern Tropical Pacific Ocean, Ryan Keith Logan

All HCAS Student Capstones, Theses, and Dissertations

Pelagic apex predators exert strong influences on ecological communities, and often support valuable commercial or recreational fisheries worldwide. Yet, due to their rarity and pelagic lifestyle, many species, such as billfishes, have proven particularly difficult to study at resolutions necessary to define dynamics of recovery from fishery interaction, physical interaction with environmental features and prey exploitation, and competitive interactions among other billfish predators. This leads to a paucity of knowledge on billfish ecology and habitat use, and hinders management efforts. With the ever-improving and miniaturization of technology and oceanographic datasets, the ability to define and quantify these interactions of fish …


Impacts On Fast-Start Performance: How Do Group Size And Habitat Degradation Alter The Escape Behavior Of A Schooling Coral Reef Fish?, Monica D. Bacchus Aug 2022

Impacts On Fast-Start Performance: How Do Group Size And Habitat Degradation Alter The Escape Behavior Of A Schooling Coral Reef Fish?, Monica D. Bacchus

All HCAS Student Capstones, Theses, and Dissertations

Escaping predation is essential for species survival, but prey must effectively match their response to the perceived threat imposed by a predator. Fish evaluate their surroundings using several sensory stimuli, including olfactory, visual, auditory, and mechanical cues. A range of taxa use the fast-start response to evade predators, including fishes, sharks, and larval amphibians. While the fast-start response (rapid bursts of swimming) is extensively studied in solitary fishes, the factors that mediate the collective escape response in schools of fish have historically been investigated far less. To address this knowledge gap, the collective escape behavior and individual escape performance of …


The Effect Of Visitor Density And Interaction On The Behavior Of Four Ray Species (Hypanus Sabina, Hypanus Say, Pseudobatos Lentiginosus, And Rhinoptera Bonasus) Housed In An Aquatic Touch Pool, Aimee Marie Little Jan 2022

The Effect Of Visitor Density And Interaction On The Behavior Of Four Ray Species (Hypanus Sabina, Hypanus Say, Pseudobatos Lentiginosus, And Rhinoptera Bonasus) Housed In An Aquatic Touch Pool, Aimee Marie Little

UNF Graduate Theses and Dissertations

Human-Animal Interactions (HAI) in zoological institutions are thought to be important in helping visitors to establish a connection with animals and thus making them more likely to contribute to conservation efforts. However, animals can respond to visitor interaction in both negative and positive ways. The growing focus on animal welfare in zoological institutions emphasizes the need for assessing different environmental inputs, including visitor interaction, and how these inputs influence behavioral outputs associated with welfare. A touch pool exhibit presents a novel interactive experience that allows visitors to directly interact with various aquatic species, including elasmobranchs, whose conservation has important implications …


Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams Jan 2022

Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams

Honors Theses and Capstones

This Senior Thesis project aimed to be able to screen samples that came in from a PhD student in Georgia for the presence of two oyster diseases, MSX and Dermo. This study was done using PCR followed by visualizing on E-gels for initial presence of disease. These were then followed by qPCR and visualization on Criterion gels. These Criterion gels were able to be analyzed in order to calculate the pg/uL of MSX and Dermo in the original ocean samples. Since the samples were sent in over the course of February-August ‘21 the presence over time was also able to …


Optimization Of Grow-Out Of Bouldering Coral Microfragments: Land Vs. Offshore Nursery, Ashlee A. Steinberg Apr 2021

Optimization Of Grow-Out Of Bouldering Coral Microfragments: Land Vs. Offshore Nursery, Ashlee A. Steinberg

All HCAS Student Capstones, Theses, and Dissertations

Corals along the Florida Reef Tract and the wider Caribbean have been declining for decades. Low densities of adult colonies hinder the ability of corals to replenish themselves through sexual reproduction, thus reef managers are focusing on restoration actions that increase coral biomass. Microfragmentation is a way to quickly increase the biomass of bouldering corals by cutting the coral into small pieces which forces the coral to allocate its resources away from reproduction and back into growth, increasing its growth rate. This study assessed the optimal location for grow-out (land vs. offshore nursery) and its synergy with size of fragment …


Distributions And Abundances Of The Red Tide Dinoflagellate Alexandrium Catenella In The Eastern Gulf Of Maine And Bay Of Fundy In Relation To Diatoms In June, July And August Of 2019, Kimberly Lina D'Adamo Aug 2020

Distributions And Abundances Of The Red Tide Dinoflagellate Alexandrium Catenella In The Eastern Gulf Of Maine And Bay Of Fundy In Relation To Diatoms In June, July And August Of 2019, Kimberly Lina D'Adamo

Electronic Theses and Dissertations

A series of three oceanographic survey cruises were conducted in June, July, and August of 2019 in the northeastern Gulf of Maine and Bay of Fundy. Surface water samples were collected and analyzed for enumerations of cell densities of the dinoflagellate Alexandrium catenella in relation to cell densities of diatoms. Hydrographic profiles of temperature, salinity, and nutrients (silicate and nitrate) were also made at each station. Data were analyzed to determine if there was any statistically significant evidence of allelopathic interference imparted by diatoms that impede A. catenella. A. catenella cells were most abundant in June, reaching 6,195 cells per …


Nearshore Vertical Distribution Of Barnacle Cyprids: Temporal Patterns And Hydrographic Variability, Gabriela Yamhure Apr 2020

Nearshore Vertical Distribution Of Barnacle Cyprids: Temporal Patterns And Hydrographic Variability, Gabriela Yamhure

Theses

Most benthic organisms living in the intertidal zone have planktonic larvae that reside temporarily in the water column before settling in their adult habitats. Larvae aggregate in offshore larval pools, and transport horizontally and vertically in the water to remain in the nearshore and during their pelagic life. While some horizontal transport of larvae can be attributed to advection, behavioral responses, like vertical swimming and buoyancy control, allow larvae to position themselves at depths where flow direction can be exploited. Thus, knowledge on how vertical larval distribution relates to physical processes can be fundamental to better understand larval transport. These …


Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara Jan 2020

Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara

Williams Honors College, Honors Research Projects

I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization. …


Physiological Response Of Elasmobrachs During Propofol Immersion, Matthew Levendosky Jan 2020

Physiological Response Of Elasmobrachs During Propofol Immersion, Matthew Levendosky

Electronic Theses and Dissertations

Sensory experiments require anesthesia so the animal is immobilized, however fish anesthetics have shown to depress sensory responses. Newer anesthetics may offer similar anesthetic relief, but differ in means of action so sensory responses may be unaffected. Propofol has been used intravenously on small elasmobranchs but may provide prolonged effects if used as an immersion anesthetic. Objectives of this study were 1. Determine appropriate concentration of anesthetic to minimize induction and recovery for animals anesthetized at a surgical plane of anesthesia and 2. Measure physiological response of the pupil to light stimuli during anesthetic immersion. To address these objectives, I …


The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli May 2019

The Effects Of Salinity On Zootechnical Performance Of Spotted Seatrout (Cynoscion Nebulosus) In Recirculating Aquaculture Systems, Eric Gigli

Master's Theses

The spotted seatrout (Cynoscion nebulosus) is a euryhaline finfish candidate for marine aquaculture in the northern Gulf of Mexico. Aquaculture of this species currently uses salinities of 25 psu or greater making production challenging in areas where high salinity water is not available. This study aimed to document the effects of salinity on zootechnical performance of spotted seatrout embryos, early larvae, and juveniles in recirculating aquaculture systems to assist with the development of protocols for low salinity culture.

Embryos obtained from a volitional spawn at 29 psu were incubated at three salinities (12.5, 18.75, or 25 psu) until …


Microplastics In Full View: Birds As Bioindicators Of Malta's Coastal Ecosystem Health, Dalton Brauer Dec 2018

Microplastics In Full View: Birds As Bioindicators Of Malta's Coastal Ecosystem Health, Dalton Brauer

Masters Theses, 2010-2019

Plastic pollution has recently become a widely studied topic, yet research on microplastics has remained lacking for specific geographic regions. Microplastics are small plastics resulting from degradation or the dumping of raw material and can lead to deleterious impacts on the coastal marine environment and its organisms. To assess Malta’s coastal environmental health, water birds (inshore, offshore and pelagic species) were used as bioindicators by assessing the presence and abundance of plastic within their stomach contents. The project hoped to fill some of the current gaps in knowledge on microplastics within Malta by creating a working baseline, as well as …


Effects Of Trophic Relationships On Oyster Reef Restoration Success In The Mississippi Sound, Virginia Robin Fleer Dec 2017

Effects Of Trophic Relationships On Oyster Reef Restoration Success In The Mississippi Sound, Virginia Robin Fleer

Dissertations

Natural and anthropogenic changes resulting from altered hydrology, hurricanes, variable precipitation, and the BP oil spill have all taken their toll on oyster reefs in Mississippi. In response, oyster reef restoration efforts are currently underway within the Northern Gulf of Mexico. In order to understand why these efforts succeed or fail, it is crucial to consider predator-prey relationships within the context of the trophic dynamics of oyster reefs. Thus, for this dissertation study I integrated a multidisciplinary approach to understanding key trophic interactions affecting oyster recruitment, growth and survival, comprising field sampling, manipulative lab experiments, and individual-based modeling. Spat settlement …


Measuring Fertilization In Populations Of Sea Scallop (Placopecten Magellanicus): Developing And Testing Methods In The Laboratory And Field, Skylar Bayer May 2017

Measuring Fertilization In Populations Of Sea Scallop (Placopecten Magellanicus): Developing And Testing Methods In The Laboratory And Field, Skylar Bayer

Electronic Theses and Dissertations

Most marine organisms are broadcast spawners, releasing their sperm and eggs into the water column. Methods of measuring in situ fertilization have proven successful with a few model species, which are reviewed in my introductory chapter. However, many commercially exploited species, such as the sea scallop Placopecten magellanicus, have been neglected. Sea scallop populations have greatly increased from fishing closures, but the mechanism behind this response is uncertain, particularly in regard to fertilization. In this dissertation I developed a methodology of measuring fertilization success and spawning events of P. magellanicus, tested it in laboratory and field settings, and …


Dna Barcoding Reveals A New Morphotype Of The Sugar Kelp, Saccharina Latissima, Dallin Brimley, Zakery Ford, Travis Gould, Brandon Guerra, Emily Haggett, Ian Jones, Elyse Johnson, Ken Peterson, Matthew Prout Apr 2017

Dna Barcoding Reveals A New Morphotype Of The Sugar Kelp, Saccharina Latissima, Dallin Brimley, Zakery Ford, Travis Gould, Brandon Guerra, Emily Haggett, Ian Jones, Elyse Johnson, Ken Peterson, Matthew Prout

Thinking Matters Symposium Archive

Phytoplankton blooms follow predictable annual cycles in the Gulf of Maine, characterized by a large spring bloom and a smaller bloom in the fall. Marine phytoplankton form the foundation of primary production in Gulf of Maine waters, and thus community changes in composition, and abundance could have cascading effects on our coastal ecosystems.

We set out to monitor the community composition, diversity, and abundance of the spring micro-phytoplankton bloom, at a Friends of Casco Bay water quality monitoring site, in South Portland, Maine. The Gulf of Maine is experiencing accelerated warming rates, and this type of monitoring can aid in …


Survey Gear Comparisons And Shark Nursery Habitat Use In Southeast Georgia Estuaries, Jeffrey Cohen Carpenter Jan 2017

Survey Gear Comparisons And Shark Nursery Habitat Use In Southeast Georgia Estuaries, Jeffrey Cohen Carpenter

UNF Graduate Theses and Dissertations

Gill nets and longlines were compared as shark nursery sampling methodologies in inshore waters of Georgia to (1) assess differences in gear selectivity, bias, and stress of capture and (2) determine potential relationships between habitat features and shark distribution and abundance. Gear selectivity varied between gears as a function of both species and life stage resulting in significantly different estimates of species and life stage compositions. Juvenile bonnetheads (Sphyrna tiburo) and young of the year blacktip sharks (Carcharhinus limbatus) experienced significantly higher stress from gill net capture than longline. Major sources of bias are thought to …


Behavior And Physiology Of Hermit Crabs During Burial: Shell Abandonment And Lactate Accumulation, Janelle Allison Shives Jun 2010

Behavior And Physiology Of Hermit Crabs During Burial: Shell Abandonment And Lactate Accumulation, Janelle Allison Shives

Loma Linda University Electronic Theses, Dissertations & Projects

Organisms living in the intertidal zone must adapt to environmental and physical stressors. One physical stressor that these organisms may face, and that may require specific behavioral and physiological responses, is burial by sediment. In this thesis I report the results of experiments in which I subjected the intertidal hermit crab, Pagurus samuelis, to burial and analyzed aspects of subsequent behavior and physiology. In the first set of experiments, hermit crabs were buried with the shell aperture facing either up or down, and at one of three depths (2, 4, or 6 cm). The factors hermit crab weight, shell …