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Full-Text Articles in Marine Biology

Isolation, Structure Elucidation, And Synthesis Of Natural Products From Marine Cyanobacteria, Keren Solomon Aug 2020

Isolation, Structure Elucidation, And Synthesis Of Natural Products From Marine Cyanobacteria, Keren Solomon

Electronic Theses and Dissertations

This thesis describes the isolation, structure elucidation, and synthesis of natural products from marine cyanobacteria. A crude extract from a cyanobacterium collected in Curacao showed selective affinity for the dopamine D5 receptor in a screen against a panel of CNS receptors. Due to the high similarity of the D5 and D1 receptor, to date there are no known ligands that differentiate them. Attempts to purify the compound responsible for this affinity led to the isolation of the known compound caylobolide A. A second extract from a cyanobacterium collected in Panama underwent bioassay-guided fractionation and yielded the novel …


Understanding The Toxicity Of Single Hydrocarbons, Oil, And Dispersed Oil: A Species Sensitivity Assessment For Five Atlantic Coral Species, Nicholas R. Turner Apr 2020

Understanding The Toxicity Of Single Hydrocarbons, Oil, And Dispersed Oil: A Species Sensitivity Assessment For Five Atlantic Coral Species, Nicholas R. Turner

HCNSO Student Theses and Dissertations

Coral reefs are keystone coastal ecosystems that are at risk of exposure to petroleum hydrocarbons from a range of sources, including oil spill incidents and chronic runoff, and are usually one of the highest valued natural resources for protection in Net Environmental Benefit Analysis (NEBA)/Spill Impact Mitigation Assessment (SIMA) of response methods and environmental damage. Previous research evaluating hydrocarbon impacts to corals has resulted in no clear characterization of sensitivity, as work has generally focused on higher-level effects, compounded by significant variability in experimental methodology. This represents an important knowledge gap in oil spill preparedness and response as it relates …


A Reexamination Of The Freshwater Mussel Community Of The Little Miami River, Emily Davis Apr 2020

A Reexamination Of The Freshwater Mussel Community Of The Little Miami River, Emily Davis

Undergraduate Honors Thesis Projects

Understanding the changes in biodiversity and local freshwater mussel populations is critical to mitigate the risks to these populations. Freshwater ecosystems continue to degrade rapidly. The freshwater mussel community of the Little Miami River is degraded and has been in decline for many years. The biodiversity contained within freshwater ecosystems is lost at unprecedented rates influenced by human activity. Freshwater mussels play a key role in understanding the effects of human activity on freshwater ecosystems as well as maintaining the health of freshwater habitats. However, they are also sensitive to changes and disturbances, in their ecosystems. This study involved monitoring …


Impacts Of The Deepwater Horizon Oil Spill On Reproduction And Ontogeny Of Gulf Killifish (Fundulus Grandis), Charles Alexander Brown Jan 2020

Impacts Of The Deepwater Horizon Oil Spill On Reproduction And Ontogeny Of Gulf Killifish (Fundulus Grandis), Charles Alexander Brown

LSU Doctoral Dissertations

On April 20, 2010, the Deepwater Horizon oil spill released an estimated 779 million liters of Macondo-252 crude oil into the Gulf of Mexico, making it the largest marine oil spill in history. Over a thousand kilometers of marshland that many species of fish use as a spawning grounds and nurseries was oiled, exposing breeding adult fish and their offspring to oil. My dissertation investigates the impacts of the Deepwater Horizon oil spill and associated remediation efforts on the reproduction and ontogeny of a sentinel ecotoxicological species, the Gulf killifish (Fundulus grandis). Concerns were raised regarding use of …


Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara Jan 2020

Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara

Williams Honors College, Honors Research Projects

I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization. …