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Microbiology

William & Mary

Theses/Dissertations

Articles 1 - 17 of 17

Full-Text Articles in Marine Biology

Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts Jan 2020

Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts

Dissertations, Theses, and Masters Projects

Cultural eutrophication, the overproduction of phytoplankton biomass in response to increased nutrient inputs directly associated with human activities, is a major threat to the health of Chesapeake Bay. Strict regulations, which require a reduction in nutrient loading from all sources, have been a key component to restoration efforts. Water reclamation facilities (WRFs), which discharge effluent containing nitrogen (N) and other nutrients into receiving waters, have implemented upgrades in an effort to comply with regulations. These improvements have decreased the concentration of highly labile dissolved inorganic N (DIN), leaving behind significant concentrations of dissolved organic N (DON) whose bioavailability, and therefore …


The Eastern Oyster Microbiome And Its Implications In The Marine Nitrogen Cycle, Ann Arfken Jan 2018

The Eastern Oyster Microbiome And Its Implications In The Marine Nitrogen Cycle, Ann Arfken

Dissertations, Theses, and Masters Projects

Microbial communities associated with a particular space or habitat, or microbiomes, play significant roles in host health and the regulation of biogeochemical cycles. In oysters these microbiomes may be important contributors in the removal of biologically available nitrogen (N) from the coastal and marine environment through the process of denitrification. Denitrification is the microbially mediated step-wise reduction of nitrate (NO3-) or nitrite (NO2-) to N2 gas. Excess nitrogen in the Chesapeake Bay has been implicated in the increase of eutrophication and other detrimental effects including harmful algal blooms, hypoxia, and loss of benthic communities. Oyster reefs have been shown to …


Abundance, Structure And Function Of Zooplankton-Associated Bacterial Communities Within The York River, Va, Samantha L. Bickel Jan 2013

Abundance, Structure And Function Of Zooplankton-Associated Bacterial Communities Within The York River, Va, Samantha L. Bickel

Dissertations, Theses, and Masters Projects

Mesozooplankton function as microbial microhabitats and can support concentrations of bacteria orders of magnitude higher than in the surrounding water. These zooplankton-associated bacteria can have much higher production rates than their free-living counterparts. Portions of the zooplankton microhabitat may also be anoxic and provide refuge for anaerobic bacteria and their associated processes within the oxygenated water column. Despite their common presence in the marine environment, zooplankton-associated bacteria are largely ignored by microbial ecologists and zooplankton ecologists alike. Consequently, factors which influence zooplanktonassociated bacterial abundance, community composition and function, and how zooplankton-associated bacteria compare to free-living bacteria are not well known. …


Iron And Carbon Limitation Of Prokaryotic Growth In The Ocean, Jacques L. Oliver Jan 2005

Iron And Carbon Limitation Of Prokaryotic Growth In The Ocean, Jacques L. Oliver

Dissertations, Theses, and Masters Projects

Studies were undertaken to examine the roles of iron and carbon in modulating prokaryotic growth in the ocean. The context of the first study was an open-open iron fertilization experiment in the high nutrient, low chlorophyll (HNLC) regime in the Southern Ocean. The context of the second study was the oligotrophic, iron-replete, and organic carbon-limited northwest Sargasso Sea. Experimental sea water cultures were amended with an iron chelator, desferrioxamine B (DFOB), and other nutrients to examine the effects of iron and carbon limitation on growth. In the first study prokaryotic abundance, carbon production, and growth rate increased in response to …


Microbial Dynamics And Biogeochemistry In The North Pacific Subtropical Gyre, Matthew J. Church Jan 2003

Microbial Dynamics And Biogeochemistry In The North Pacific Subtropical Gyre, Matthew J. Church

Dissertations, Theses, and Masters Projects

The research presented in this dissertation describes the influence of planktonic bacterial growth on upper ocean organic matter dynamics in the North Pacific Subtropical Gyre (NPSG). Examination of the temporal dynamics in dissolved organic matter (DOM) was coupled with investigations that targeted the influence of heterotrophic bacterial production (HBP) on organic matter fluxes in the NPSG. Nine cruises to the Hawaii Ocean Time-series field site Station ALOHA revealed that HBP accounted for a large flux of organic carbon in the upper ocean of the NPSG. HBP was significantly enhanced by sunlight, with photoenhancement of HBP accounting for 3.2 mol C …


Bacterial Properties And Dissolved Organic Matter Distributions In The Black Sea, Jessica A. Morgan Jan 2003

Bacterial Properties And Dissolved Organic Matter Distributions In The Black Sea, Jessica A. Morgan

Dissertations, Theses, and Masters Projects

No abstract provided.


Molecular Characterization Of Cultured Perkinsus Marinus Isolates, Gwynne D. Brown Jan 2001

Molecular Characterization Of Cultured Perkinsus Marinus Isolates, Gwynne D. Brown

Dissertations, Theses, and Masters Projects

Perkinsus marinus is the causative agent of the oyster disease Dermo in the eastern oyster, Crassostrea virginica. In vitro propagation of the parasite has led to the establishment of multiple isolates by several investigators. Little work, however, has been done to characterize different isolates. In this study multiple isolates were examined for genetic and biochemical diversity. at two loci, the ITS region and the ATAN region, there was as much intra-isolate genetic variation among DNA sequences of some isolates as there was inter-isolate variation. Variation was also observed at a third loci, a subtilisin-like serine protease gene. This is the …


Bacterial Dynamics And Community Structure In The York River Estuary, Gary Edward Schultz Jr Jan 1999

Bacterial Dynamics And Community Structure In The York River Estuary, Gary Edward Schultz Jr

Dissertations, Theses, and Masters Projects

Bacterial community dynamics were investigated over seasonal and basin scales within the York River, VA, estuary. Various parameters characterizing bacterioplankton dynamics were measured weekly at a single station (March 1996 through May 1997) and monthly at six stations (June 1996 through May 1997) spanning the entire salinity gradient (0 - ca. 20 psu over 60 km). Bacterial abundance and production were found to be high throughout the estuary. Bacterial abundance ranged from 4.4 x 108 to 1.3 x 1010 cells-liter-1Incorporation of 3H-thymidine ranged from 10 to 863 pmol-liter-1-hr-1 while 3H-leucine incorporation rates ranged from 25 to 1963 pmol-liter -1-hr-1. Clear …


Carbon Production And Growth Physiology Of Heterotrophic Bacteria In A Subtropical Coral Reef Ecosystem, Peter Dylan Countway Jan 1999

Carbon Production And Growth Physiology Of Heterotrophic Bacteria In A Subtropical Coral Reef Ecosystem, Peter Dylan Countway

Dissertations, Theses, and Masters Projects

No abstract provided.


Limitation Of Bacterial Growth By Dissolved Organic Matter And Iron In The Southern Ocean, Matthew J. Church Jan 1999

Limitation Of Bacterial Growth By Dissolved Organic Matter And Iron In The Southern Ocean, Matthew J. Church

Dissertations, Theses, and Masters Projects

No abstract provided.


Effects Of Sunlight And Seasonal Temperature On Persistence Of Four Bacteriophages In An Estuarine Environment, Terri A. Muller Jan 1995

Effects Of Sunlight And Seasonal Temperature On Persistence Of Four Bacteriophages In An Estuarine Environment, Terri A. Muller

Dissertations, Theses, and Masters Projects

No abstract provided.


Effects Of Simulated Sediment Resuspension Events On The Abundance Of Water Column Bacteria Of Tomales Bay, California, Christopher J. Collumb Jan 1995

Effects Of Simulated Sediment Resuspension Events On The Abundance Of Water Column Bacteria Of Tomales Bay, California, Christopher J. Collumb

Dissertations, Theses, and Masters Projects

No abstract provided.


Growth Rate And Grazing Dynamics Of Coccoid Cyanobacteria In The Lower Chesapeake Bay, Carol J. Falkenhayn Jan 1990

Growth Rate And Grazing Dynamics Of Coccoid Cyanobacteria In The Lower Chesapeake Bay, Carol J. Falkenhayn

Dissertations, Theses, and Masters Projects

No abstract provided.


Frna Coliphages As A Viral Indicator Of Sewage Pollution In An Estuary, David Miller Boyd Jan 1990

Frna Coliphages As A Viral Indicator Of Sewage Pollution In An Estuary, David Miller Boyd

Dissertations, Theses, and Masters Projects

No abstract provided.


Heterotrophic Bacterial Production: Relationships To Biological And Abiological Factors In Estuarine Environments, Eric Thor Koepfler Jan 1989

Heterotrophic Bacterial Production: Relationships To Biological And Abiological Factors In Estuarine Environments, Eric Thor Koepfler

Dissertations, Theses, and Masters Projects

Section 1. Ecotoxicological effects of creosote contamination on benthic bacterial communities in the Elizabeth River, Virginia were investigated using both structural and functional microbial parameters. Results indicated that cell specific and total heterotrophic bacterial production parameters were depressed in a dose dependent manner with increasing sediment PAH concentrations. Toxicity effects upon production were modified by temporal trends associated with temperature as well as spatial sediment characteristics. of the parameters employed, the tritiated thymidine production assay was found to be the most sensitive for detection of ecotoxicological effects. Section 2. Bacterial abundance and production were examined during a destratification event in …


Nanoplankton Dynamics In A Salt Marsh-Mudflat Ecosystem, Helena Maria Galvaeo Jan 1984

Nanoplankton Dynamics In A Salt Marsh-Mudflat Ecosystem, Helena Maria Galvaeo

Dissertations, Theses, and Masters Projects

No abstract provided.


Chitinoclastic Bacteria From The Mid-Atlantic Shelf: Distribution, Taxonomy, And Effect Of Oil On Growth And Chitin Degradation, Darbie Anne Lister Jan 1979

Chitinoclastic Bacteria From The Mid-Atlantic Shelf: Distribution, Taxonomy, And Effect Of Oil On Growth And Chitin Degradation, Darbie Anne Lister

Dissertations, Theses, and Masters Projects

No abstract provided.