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Full-Text Articles in Marine Biology
The Sponge Microbiome Project, Lucas Moitinho-Silva, Shaun Nielsen, Amnon Amir, Antonio Gonzalez, Gail Ackermann, Carlo Cerrano, Carmen Astudillo-Garcia, Cole Easson, Detmer Sipkema, Fang Liu, Georg Steinert, Giorgos Kotoulas, Grace Mccormack, Guofang Feng, James J. Bell, Jan Vicente, Johannes R. Bjork, Jose M. Montoya, Julie B. Olson, Julie Reveillaud, Laura Steindler, Mari-Carmen Pineda, Maria V. Marra, Micha Ilan, Michael W. Taylor, Paraskevi Polymenakou, Patrick M. Erwin, Peter J. Schupp, Rachel L. Simister, Rob Knight, Robert W. Thacker, Rodrigo Costa, Russell T. Hill, Susanna Lopez-Legentil, Thanos Dailianis, Timothy Ravasi, Ute Hentschel, Zhiyong Li, Nicole S. Webster, Torsten Thomas
The Sponge Microbiome Project, Lucas Moitinho-Silva, Shaun Nielsen, Amnon Amir, Antonio Gonzalez, Gail Ackermann, Carlo Cerrano, Carmen Astudillo-Garcia, Cole Easson, Detmer Sipkema, Fang Liu, Georg Steinert, Giorgos Kotoulas, Grace Mccormack, Guofang Feng, James J. Bell, Jan Vicente, Johannes R. Bjork, Jose M. Montoya, Julie B. Olson, Julie Reveillaud, Laura Steindler, Mari-Carmen Pineda, Maria V. Marra, Micha Ilan, Michael W. Taylor, Paraskevi Polymenakou, Patrick M. Erwin, Peter J. Schupp, Rachel L. Simister, Rob Knight, Robert W. Thacker, Rodrigo Costa, Russell T. Hill, Susanna Lopez-Legentil, Thanos Dailianis, Timothy Ravasi, Ute Hentschel, Zhiyong Li, Nicole S. Webster, Torsten Thomas
Marine & Environmental Sciences Faculty Articles
Marine sponges (phylum Porifera) are a diverse, phylogenetically deep-branching clade known for forming intimate partnerships with complex communities of microorganisms. To date, 16S rRNA gene sequencing studies have largely utilised different extraction and amplification methodologies to target the microbial communities of a limited number of sponge species, severely limiting comparative analyses of sponge microbial diversity and structure. Here, we provide an extensive and standardised dataset that will facilitate sponge microbiome comparisons across large spatial, temporal, and environmental scales. Samples from marine sponges (n = 3569 specimens), seawater (n = 370), marine sediments (n = 65) and other …
Microbiome And Bacterioplankton Rrna Gene Sequence Data Collected From Gulf Of Mexico Seawater Samples, Cruises Dp01 And Dp02 From May 2015 - August 2015, Cole Easson, Jose V. Lopez
Microbiome And Bacterioplankton Rrna Gene Sequence Data Collected From Gulf Of Mexico Seawater Samples, Cruises Dp01 And Dp02 From May 2015 - August 2015, Cole Easson, Jose V. Lopez
DEEPEND Datasets
Seawater was collected and filtered for microbiome and bacterioplankton sequencing and analyses at various depths during two planned DEEPEND cruise expeditions to the GOM. Filters were stored and then processed for total environmental genomic DNA according to standard methods (see earthmicrobiome.org). 16S rRNA amplicon libraries covering the V4 hypervariable region was generated with universal PCR primers and then sequenced on an Illumina MiSeq DNA sequencing platform. Bioinformatics analyses in QIIME and R was used to quality filter and assess microbial community diversity and taxonomic composition. These baseline characterizations track alpha and beta diversity at different depths ranging from 0 – …