Marine Biology Commons^™

Effects Of Methyl Parathion On The Cellular Immune Responses In Giant Black Tiger Shrimp, Penaeus Monodon, Nantarika. Bodhipaksha

Dissertations, Theses, and Masters Projects

The effects of an organophosphorus pesticide, methyl parathion, on cellular immune defense mechanisms of the giant black tiger shrimp (Penaeus monodon) were studied. Animals were exposed for 96 hours at concentrations equal to, above and below the LC&\sb{lcub}50{rcub}& (3 ppb). Phagocytes were obtained from the heart and circulating hemolymph. Cellular immune responses, including chemotaxis, phagocytosis and the respiratory burst were examined. Chemotactic activity was determined by means of a modified Boyden double chamber technique. There was a dose-dependent decrease in the chemotactic activities of both circulating hemocytes and cardiac phagocytes. Phagocytic activity (percent phagocytosis) was examined by the microscopic enumeration …