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Activation Of Amp-Activated Protein Kinase As An Early Indicator For Stress In The Lobster, Homarus Americanus, Stephanie Podolski
Activation Of Amp-Activated Protein Kinase As An Early Indicator For Stress In The Lobster, Homarus Americanus, Stephanie Podolski
All Theses And Dissertations
Variations in water temperature, salinity, pH, and oxygen concentration are stressors that marine invertebrates face on a daily basis. Each of these physiological stressors creates a large cellular demand for energy. In mammals, energy metabolism is regulated by the enzyme AMP-activated protein kinase (AMPK), which is highly conserved during evolution. This project was designed to test the hypothesis that AMPK is present and activated by temperature, hypoxia, and anoxia stress in the lobster, Homarus americanus.
Animals were exposed to a rapid and progressive increase in temperature (6ºC per hour) beginning at 14 ºC. We measured lactate concentrations and AMPK …