Marine Biology Commons^™

Multi-System Analysis Of Nitrogen Use By Phytoplankton And Heterotrophic Bacteria, Paul B. Bradley

Dissertations, Theses, and Masters Projects

Traditional measurements of phytoplankton N uptake have been confounded by bacterial retention on filters used in 15N uptake studies, and such methodological obstacles have limited our understanding of phytoplankton-bacterial interactions regarding N cycling. In this research, uptake of various inorganic and organic N substrates by phytoplankton and bacteria was measured in several marine ecosystems using two distinct approaches: size fractionation into phytoplankton and bacterial size classes, and flow cytometric (FCM) sorting of autotrophic cells. Comprehensive assessments of N uptake dynamics were conducted in Chesapeake Bay, the Mid-Atlantic Bight, and Raunefjord, Norway, with supplementary data collected from the York River, Virginia …