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Articles 1 - 14 of 14
Full-Text Articles in Marine Biology
Neutral Genetic Variation In Adult Chinook Salmon (Oncorhynchus Tshawytscha) Affects Brain-To-Body Trade-Off And Brain Laterality, Mallory L. Wiper, Sarah J. Lehnert, Daniel D. Heath, Dennis M. Higgs
Neutral Genetic Variation In Adult Chinook Salmon (Oncorhynchus Tshawytscha) Affects Brain-To-Body Trade-Off And Brain Laterality, Mallory L. Wiper, Sarah J. Lehnert, Daniel D. Heath, Dennis M. Higgs
Great Lakes Institute for Environmental Research Publications
Low levels of heterozygosity can have detrimental effects on life history and growth characteristics of organisms but more subtle effects such as those on trade-offs of expensive tissues and morphological laterality, especially of the brain, have not been explicitly tested. The objective of the current study was to investigate how estimated differences in heterozygosity may potentially affect brain-to-body trade-offs and to explore how these heterozygosity differences may affect differential brain growth, focusing on directional asymmetry in adult Chinook salmon (Oncorhynchus tshawytscha) using the laterality and absolute laterality indices. Level of inbreeding was estimated as mean microsatellite heterozygosity resulting …
Nuclear Genome Size Diversity Of Marine Invertebrate Taxa Using Flow Cytometric Analysis, Kyle Roebuck
Nuclear Genome Size Diversity Of Marine Invertebrate Taxa Using Flow Cytometric Analysis, Kyle Roebuck
HCNSO Student Theses and Dissertations
Genomic analysis provides a substantial amount of information on evolutionary history, novel genes, transcriptomic expression and regulation in response to environmental stimuli, how efficiently organisms utilize their genome, and directional genome evolution. Genome size analysis serves as the first step in the sequencing process, because sequencing and annotation costs are directly correlated with genome size. Invertebrates represent the vast majority of faunal diversity on the planet, and, to a greater extent, the marine environment, although they are vastly understudied when compared to vertebrate genomes. Flow cytometry is a widely used, reliable, and accurate means of estimating genome sizes and has …
Bioinformatic And Experimental Approaches For Deeper Metaproteomic Characterization Of Complex Environmental Samples, Ramsunder Mahadevan Iyer
Bioinformatic And Experimental Approaches For Deeper Metaproteomic Characterization Of Complex Environmental Samples, Ramsunder Mahadevan Iyer
Doctoral Dissertations
The coupling of high performance multi-dimensional liquid chromatography and tandem mass spectrometry for characterization of microbial proteins from complex environmental samples has paved the way for a new era in scientific discovery. The field of metaproteomics, which is the study of protein suite of all the organisms in a biological system, has taken a tremendous leap with the introduction of high-throughput proteomics. However, with corresponding increase in sample complexity, novel challenges have been raised with respect to efficient peptide separation via chromatography and bioinformatic analysis of the resulting high throughput data. In this dissertation, various aspects of metaproteomic characterization, including …
Environmental Dna Detection Of Rare And Invasive Fish Species In Two Great Lakes Tributaries, Katherine D. Balasingham, Ryan P. Walter, Nicholas E. Mandrak, Daniel D. Heath
Environmental Dna Detection Of Rare And Invasive Fish Species In Two Great Lakes Tributaries, Katherine D. Balasingham, Ryan P. Walter, Nicholas E. Mandrak, Daniel D. Heath
Great Lakes Institute for Environmental Research Publications
The extraction and characterization of DNA from aquatic environmental samples offers an alternative, non-invasive approach for the detection of rare species. Environmental DNA, coupled with PCR and next-generation sequencing (“metabarcoding”), has proven to be very sensitive for the detection of rare aquatic species. Our study used a custom designed group-specific primer set and next-generation sequencing for the detection of three species at risk; (Eastern Sand Darter, Ammocrypta pellucida; Northern Madtom, Noturus stigmosus; and Silver Shiner, Notropis photogenis), one invasive species (Round Goby, Neogobius melanostomus) and an additional 78 native species from two large Great Lakes tributary rivers in southern Ontario, …
Environmental And Genetic Determinants Of Transcriptional Plasticity In Chinook Salmon, Kyle W. Wellband, John W. Heath, Daniel D. Heath
Environmental And Genetic Determinants Of Transcriptional Plasticity In Chinook Salmon, Kyle W. Wellband, John W. Heath, Daniel D. Heath
Great Lakes Institute for Environmental Research Publications
Variation in gene transcription is widely believed to be the mechanistic basis of phenotypically plastic traits; however, comparatively little is known about the inheritance patterns of transcriptional variation that would allow us to predict its response to selection. In addition, acclimation to different environmental conditions influences acute transcriptional responses to stress and it is unclear if these effects are heritable. To address these gaps in knowledge, we assayed levels of messenger RNA for 14 candidate genes at rest and in response to a 24-h confinement stress for 72 half-sib families of Chinook salmon reared in two different environments (hatchery and …
Differential Invasion Success In Aquatic Invasive Species: The Role Of Within- And Among-Population Genetic Diversity, Kyle W. Wellband, Harri Pettitt-Wade, Aaron T. Fisk, Daniel D. Heath
Differential Invasion Success In Aquatic Invasive Species: The Role Of Within- And Among-Population Genetic Diversity, Kyle W. Wellband, Harri Pettitt-Wade, Aaron T. Fisk, Daniel D. Heath
Great Lakes Institute for Environmental Research Publications
© 2017, Springer International Publishing AG Switzerland. Despite a well-developed theoretical basis for the role of genetic diversity in the colonization process, contemporary investigations of genetic diversity in biological invasions have downplayed its importance. Observed reductions in genetic diversity have been argued to have a limited effect on the success of establishment and impact based on empirical studies; however, those studies rarely include assessment of failed or comparatively less-successful biological invasions. We address this gap by comparing genetic diversity at microsatellite loci for taxonomically and geographically paired aquatic invasive species. Our four species pairs contain one highly successful and one …
Bioaccumulation Of Polychlorinated Biphenyls (Pcbs) In Atlantic Sea Bream (Archosargus Rhomboidalis) From Kingston Harbour, Jamaica, Ann-Teneil O'Connor, Dwight Robinson, Tara P. Dasgupta, Aaron T. Fisk, Ken G. Drouillard
Bioaccumulation Of Polychlorinated Biphenyls (Pcbs) In Atlantic Sea Bream (Archosargus Rhomboidalis) From Kingston Harbour, Jamaica, Ann-Teneil O'Connor, Dwight Robinson, Tara P. Dasgupta, Aaron T. Fisk, Ken G. Drouillard
Great Lakes Institute for Environmental Research Publications
Multiple sizes of Sea bream were collected from Kingston Harbour, Jamaica, to assess steady state bioaccumulation of polychlorinated biphenyls (PCBs) in a tropical fish. Sea beam fork lengths ranged from 7.3-21.5 cm (n=36 fish) and tissue lipids decreased with body length. Larger fish had lower δ13C isotopes compared to smaller fish, suggesting a change in diet. Linear regressions showed no differences in lipid equivalent sum PCB concentrations with size. However, differences in individual congener bioaccumulation trajectories occurred. Less hydrophobic PCBs decreased with increasing body length, intermediate PCBs showed no trend, whereas highly hydrophobic (above log KOW of …
Transcriptomic And Epigenetic Responses To Environmental Stress In Marine Bivalves With A Focus On Harmful Algal Blooms, Maria Victoria Suarez Ulloa
Transcriptomic And Epigenetic Responses To Environmental Stress In Marine Bivalves With A Focus On Harmful Algal Blooms, Maria Victoria Suarez Ulloa
FIU Electronic Theses and Dissertations
Global change poses new threats for life in the oceans forcing marine organisms to respond through molecular acclimatory and adaptive strategies. Although bivalve molluscs are particularly tolerant and resilient to environmental stress, they must now face the challenge of more frequent and severe Harmful Algal Blooms (HABs) episodes. These massive outbreaks of microalgae produce toxins that accumulate in the tissues of these filter-feeder organisms, causing changes in their gene expression profiles, which in turn modify their phenotype in order to maintain homeostasis. Such modifications in gene expression are modulated by epigenetic mechanisms elicited by specific environmental stimuli, laying the foundations …
Divergent Responses Of Larval And Juvenile Blue Mussels To Low Salinity Exposure, Melissa A. May
Divergent Responses Of Larval And Juvenile Blue Mussels To Low Salinity Exposure, Melissa A. May
Electronic Theses and Dissertations
In this study, we compared the osmotic stress response of larval and juvenile blue mussels (Mytilus edulis) at the transcriptomic, metabolomic, and whole organism levels. Blue mussels inhabit coastal areas, where they face climate-induced reductions in nearshore salinity. Despite their ecological and economic importance, scientists do not fully understand the underlying transcriptomic and cellular mechanisms of the osmotic stress response in blue mussels or how the ability to respond to stress changes throughout development. Blue mussels spend the first weeks of life developing through several larval stages in the plankton. These early life history stages are more vulnerable …
Characterization Of Andrenocortical Tissue Morphology, Histology, And Steroid Synthesis Among Finetooth (Carcharhinus Isodon), Blacktip (Carcharhinus Limbatus), Atlantic Sharpnose (Rhizoprionodon Terraenovae), And Bonnethead (Sphyrna Tiburo) Sharks, Danielle Elizabeth Bailey
Master's Theses
Adrenocortical (interrenal) tissue is composed of steroidogenic cells that produce corticosteroids involved in the stress response and hydromineral balance. Previous research characterizing the elasmobranch interrenal suggests that the number of interrenal bodies that produce the single primary corticosteroid, 1a-hydroxycorticosterone (1a-OHB), varies among species. However, potential species-specific differences in the amount of interrenal tissue and major steroid products produced have been understudied. To address this critical gap in our understanding of elasmobranch biology, this study examined interrenal morphology, cell structure, steroidogenic enzyme distribution (3bHSD), and steroid production in four shark species: Finetooth Carcharhinus isodon, Blacktip Carcharhinus limbatus, Atlantic Sharpnose …
Conservation Physiology And The Quest For A ‘Good’ Anthropocene, Christine L. Madliger, Craig E. Franklin, Kevin R. Hultine, Mark Van Kleunen, Robert J. Lennox, Oliver P. Love, Jodie L. Rummer, Steven J. Cooke
Conservation Physiology And The Quest For A ‘Good’ Anthropocene, Christine L. Madliger, Craig E. Franklin, Kevin R. Hultine, Mark Van Kleunen, Robert J. Lennox, Oliver P. Love, Jodie L. Rummer, Steven J. Cooke
Great Lakes Institute for Environmental Research Publications
It has been proposed that we are now living in a new geological epoch known as the Anthropocene, which is specifically defined by the impacts that humans are having on the Earth’s biological diversity and geology. Although the proposal of this term was borne out of an acknowledgement of the negative changes we are imparting on the globe (e.g. climate change, pollution, coastal erosion, species extinctions), there has recently been action amongst a variety of disciplines aimed at achieving a ‘good Anthropocene’ that strives to balance societal needs and the preservation of the natural world. Here, we outline ways that …
Environmental Pseudomonads Inhibit Cystic Fibrosis Patient-Derived Pseudomonas Aeruginosa, Payel Chatterjee, Elizabeth Davis, Fengan Yu, Sarah James, Julia H. Wildschutte, Daniel D. Wiegmann, David H. Sherman, Robert M. Mckay, John J. Lipuma, Hans Wildschutte
Environmental Pseudomonads Inhibit Cystic Fibrosis Patient-Derived Pseudomonas Aeruginosa, Payel Chatterjee, Elizabeth Davis, Fengan Yu, Sarah James, Julia H. Wildschutte, Daniel D. Wiegmann, David H. Sherman, Robert M. Mckay, John J. Lipuma, Hans Wildschutte
Great Lakes Institute for Environmental Research Publications
Pseudomonas aeruginosa is an opportunistic pathogen which is evolving resistance to many currently used antibiotics. While much research has been devoted to the roles of pathogenic P. aeruginosa in cystic fibrosis (CF) patients, less is known of its ecological properties. P. aeruginosa dominates the lungs during chronic infection in CF patients, yet its abundance in some environments is less than that of other diverse groups of pseudomonads. Here, we sought to determine if clinical isolates of P. aeruginosa are vulnerable to environmental pseudomonads that dominate soil and water habitats in one-to-one competitions which may provide a source of inhibitory factors. …
Frontiers In Otolith Chemistry: Insights, Advances And Applications, B. D. Walther, K. E. Limburg, C. M. Jones, J. J. Schaffler
Frontiers In Otolith Chemistry: Insights, Advances And Applications, B. D. Walther, K. E. Limburg, C. M. Jones, J. J. Schaffler
OES Faculty Publications
The rapid proliferation of publications employing chemical assays of fish hard parts, and otoliths in particular, has led to many novel insights into the migration patterns, life history strategies and mixed stock dynamics of fishes across the globe (Campana et al., 2000; Elsdon et al., 2008; Walther & Limburg, 2012). These insights include uncovering diverse migratory strategies within species and populations (Kerr et al., 2009; Hogan et al., 2014; Schoen et al., 2016), quantifying rates of mixing among stocks across management boundaries (Rooker et al., 2008; Walther & Thorrold, 2010) and estimating the relative …
Beyond Zar: The Use And Abuse Of Classification Statistics For Otolith Chemistry, C. M. Jones, M. Palmers, J. J. Schaffler
Beyond Zar: The Use And Abuse Of Classification Statistics For Otolith Chemistry, C. M. Jones, M. Palmers, J. J. Schaffler
OES Faculty Publications
Classification method performance was evaluated using otolith chemistry of juvenile Atlantic menhaden Brevoortia tyrannus when assumptions of data normality were met and were violated. Four methods were tested [linear discriminant function analysis (LDFA), quadratic discriminant function analysis (QDFA), random forest (RF) and artificial neural networks (ANN)] using computer simulation to determine their performance when variable-group means ranged from small to large and their performance under conditions of typical skewness to double the amount of skewness typically observed. Using the kappa index, the parametric methods performed best after applying appropriate data transformation, gaining 2% better performance with LDFA performing slightly better …