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Full-Text Articles in Marine Biology
Investigations Into Aldefluor As A Novel Method For Identifying Leukemia In Soft-Shell Clams, Katherine F. Norwood
Investigations Into Aldefluor As A Novel Method For Identifying Leukemia In Soft-Shell Clams, Katherine F. Norwood
Student Research Projects
The clam species Mya arenaria is a common model organism in leukemia research. The current method for classifying the degree of cancer progression is by examining cell morphology with light microscopy. This approach is highly qualitative, which makes differentiation of pre-leukemic and semi-leukemic individuals difficult. One quantitative approach that may differentiate individuals is based on levels of aldehyde dehydrogenase (ALDH) expression. The enzyme assay ALDEFLUOR® can actively measure ALDH expression in viable cells, but the effectiveness of certain protocol conditions is dependent upon the cell type.
Investigations Into The Use Of Aldefluor® As An Alternative Method To Classify Leukemia In The Soft-Shell Clam Mya Arenaria., Katherine F. Norwood
Investigations Into The Use Of Aldefluor® As An Alternative Method To Classify Leukemia In The Soft-Shell Clam Mya Arenaria., Katherine F. Norwood
Student Research Projects
The soft-shell clam species Mya arenaria is a common model organism in leukemia research. Currently, the typical method for classifying leukemic from non-leukemic specimens is based on visualization of cell concentration and morphology through light microscopy. This approach is highly qualitative, which makes identification of pre-leukemic and semi-leukemic specimens difficult. One more quantitative approach uses flow cytometry to measure levels of aldehyde dehydrogenase (ALDH) expression. In certain human tissues, such as breast tissue, high ALDH expression is a marker for identifying cancer stem cells. The enzyme assay ALDEFLUOR® can actively measure aldehyde dehydrogenase expression in viable cells, but the …