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Full-Text Articles in Laboratory and Basic Science Research
Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati
Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati
Rajesh Pati
A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.
Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati
Micropropagation Of Mangifera Indica L. Cv. Kurakkan Through Somatic Embryogenesis, Rajesh Pati
Rajesh Pati
Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4- D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15oC. Somatic embryos were matured on modified MS medium fortified with …