Laboratory and Basic Science Research Commons^™

Modeling Biphasic, Non-Sigmoidal Dose-Response Relationships: Comparison Of Brain- Cousens And Cedergreen Models For A Biochemical Dataset, Venkat D. Abbaraju, Tamaraty L. Robinson, Brian P. Weiser

Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship

Biphasic, non-sigmoidal dose-response relationships are frequently observed in biochemistry and pharmacology, but they are not always analyzed with appropriate statistical methods. Here, we examine curve fitting methods for “hormetic” dose-response relationships where low and high doses of an effector produce opposite responses. We provide the full dataset used for modeling, and we provide the code for analyzing the dataset in SAS using two established mathematical models of hormesis, the Brain-Cousens model and the Cedergreen model. We show how to obtain and interpret curve parameters such as the ED50 that arise from modeling, and we discuss how curve parameters might change …

The Impact Of Natural Rubber As A Toughening Agent On The Strength, Degradability, And Toxicity Of An Algae-Based Bioplastic, Megan Driscoll

Honors College

With the growing attention on widespread plastic usage and its impact on the environment and human health, the need for sustainable alternatives to petroleum-based plastics is more important than ever. One of the most promising solutions is bioplastics; however, current bioplastics struggle to compete with the material properties of petroleum- based plastics. Agar is a sustainable algae-derived hydrocolloid polysaccharide that can be used for bioplastics and biofilms. Despite promising characteristics, bioplastics made from agar are brittle. Common additives, such as the plasticizer glycerol, offset brittleness but sacrifice strength in return. This study looks at the impact of natural rubber as …

Great East Lake 2021 Water Quality Highlight Report, Amanda Mcquaid, Robert Craycraft

UNH Cooperative Extension

A Water Quality Highlight Report by UNH CE Lakes Lay Monitoring Program for Great East Lake (Wakefield, NH; Acton , ME) for sampling efforts in 2021.

Belleau Lake 2021 Water Quality Highlight Report, Amanda Mcquaid, Robert Craycraft

UNH Cooperative Extension

A Water Quality Highlight Report by UNH CE Lakes Lay Monitoring Program for Belleau Lake in Wakefield, NH for 2021.

Balch Pond 2021 Water Quality Highlight Report, Amanda Mcquaid, Robert Craycraft

UNH Cooperative Extension

A Water Quality Highlight Report by UNH CE Lakes Lay Monitoring Program for Balch Pond (Wakefield, NH; Acton and Newfield, ME) for sampling efforts up to 2021.

Lingering Impacts Of Hurricane Hugo On Rhizophora Mangle (Red Mangrove) Population Genetics On St. John, Usvi, Paul Ax Bologna, James J. Campanella, Dena J. Restaino, Zachary A. Fetske, Matthew Lourenco, John V. Smalley

Marine Biology and Coastal Sciences Faculty Scholarship and Creative Works

Stochastic events can have catastrophic effects on island populations through a series of genetic stressors from reduced population size. We investigated five populations of red mangrove (Rhizophora mangle) from St. John, USVI, an UNESCO Biosphere Reserve, which were impacted by Hurricane Hugo in 1989. Our goal was to determine diversity and to ascertain potential population bottlenecks two decades after the event. With the lowest observed heterozygosity, highest inbreeding coefficient, and evidence of a major bottleneck, our results demonstrated that the Great Lameshur mangroves, devastated by Hurricane Hugo, were the least diverse stand of trees. The other four populations from St. …

The Effects Of Copper Exposure On Fish Locomotion And Predator-Prey Interactions, Tiffany N. Yanez

FIU Electronic Theses and Dissertations

This study determined the effects of the copper water quality criterion (WQC) by the EPA on [1] swimming performance and [2] predator-prey interactions of the Sailfin Molly, across a salinity gradient. Fish acclimated to FW (0 ppt) and 8-ppt saltwater were exposed to 11.3 and 8.44 µg/L Cu for 96 h, respectively. At the end of the exposures, fish swimming performance was determined by using the critical swimming speed, U_crit, the speed at which a fish cannot longer maintain position in the water column. U_critwas then measured again after a 4-week depuration period to determine if …

Development And Application Of Aquatic Toxicology Studies For The Assessment Of Impacts Due To Chemical Stressors Using Non-Standard Indigenous Organisms, Abraham Jeffrey Smith

FIU Electronic Theses and Dissertations

Research in the multidisciplinary science of ecotoxicology is crucial to assess injuries to ecosystem resources from chemical spills or other stressors used to support environmental decision-making. Established guidelines recommend the use of non-standard native species in toxicity investigations. This work focused on the use of native species for aquatic toxicity assessment to make more relevant conclusions on the potential for adverse biological effects to occur as a result to single chemical exposures or exposures to a complex mixture like oil. We apply these studies to investigate petroleum product impacts from the Deepwater Horizon incident and concerns for metal toxicity in …

The Effect Of Target-Specific Biomolecules In Breast Cancer, Mohannad Garoub

FIU Electronic Theses and Dissertations

Cancer is the second leading cause of mortality in the United States and the World, therefore, early effective prevention, diagnosis, and therapy is needed. Estrogens play a major role in the initiation and progression of breast cancer. Elevated lifetime exposure to estrogens is associated with an increased risk of developing breast cancer. Estrogens through influencing mitochondria contribute to estrogen induced breast carcinogenesis; however, the exact mitochondrial mechanisms underlying the estrogen carcinogenic effect in breast tissue are not clearly understood. For this dissertation, the mitotoxic and cytotoxic effects of triphenylphosphonium cation (TPP) and Origanum majorana organic extract (OME) as well as …

Survival Model Of Intrahepatic Cholangiocarcinoma; Sex As A Biological Variable, Mary A. Phillippi, Justin L. Mott, Cody J. Wehrkamp, Ying Xie, David Oupicky, Ashley M. Mohr, Bailey A. Stringham

Hepatobiliary Cancers: Pathobiology and Translational Advances

No abstract provided.

Live And Dead Coral Cover On Three Reefs And Public Perceptions Of Degradation Around Almirante Bay, Bocas Del Toro, Phoebe Thompson

Independent Study Project (ISP) Collection

Almirante Bay is a lagoonal body of water off the western Caribbean coast of Panama. The islands within the bay are popular tourist destinations, drawing visitors from around the world to explore beaches and coral reefs. However, the reefs in Almirante Bay are continually exposed to both natural and anthropogenic stressors, such as temperature inversions, boat pollution, sedimentation from dredging, and chemical runoff from coastal banana plantations. Because the economy of the area is heavily dependent on tourism, this creates an interesting dynamic in that the main source of income (tourism) could be detrimental to a main attraction of the …

Tom Curran, Phd, Frs, Named Executive Director Of Children's Research Institute, Children's Mercy Hospital

Our Story Continues

Tom Curran, PhD, FRS, has been named Chief Scientific Officer and Executive Director of the Children’s Mercy Children’s Research Institute (CRI), effective Feb. 1 [2016]

The Children’s Research Institute at Children’s Mercy Kansas City was established last year to build on the hospital’s century-long history in pediatric research and to focus the hospital’s future research efforts in four key areas:

• Pediatric Genomic Medicine
• Clinical Pharmacology
• Health Services and Outcomes
• Health Care Delivery

Microbial Communities Of The Providence River, Jacqueline Kratch

Honors Projects in Science and Technology

The Providence River has been industrialized for over one-hundred years. Industries such as oil storage and metal recycling facilities have left high levels of pollutant metals, including lead (Pb), in the soil and water. The elevated Pb concentrations in these environments influence the selection of Pb resistance mechanisms in the bacterial community1. One mechanism of heavy metal resistance is the Pb efflux pump, consisting of proteins in the cell membrane that aid in the transport of Pb out of the cell2. In this study we investigated the co-occurrence of Pb efflux pumps and antibiotic efflux pumps in bacteria from Pb …

Brief Embryonic Strychnine Exposure In Zebrafish Causes Long-Term Adult Behavioral Impairment With Indications Of Embyronic Synaptic Changes, Nicole M. Roy, Brianna Arpie, Joseph Lugo, Elwood Linney, Edward D. Levin, Daniel Cerutti

Biology Faculty Publications

Zebrafish provide a powerful model of the impacts of embryonic toxicant exposure on neural development that may result in long-term behavioral dysfunction. In this study, zebrafish embryos were treated with 1.5mM strychnine for short embryonic time windows to induce transient changes in inhibitory neural signaling, and were subsequently raised in untreated water until adulthood. PCR analysis showed indications that strychnine exposure altered expression of some genes related to glycinergic, GABAergic and glutamatergic neuronal synapses during embryonic development. In adulthood, treated fish showed significant changes in swimming speed and tank diving behavior compared to controls. Taken together, these data show that …

Ppar Agonists Down-Regulate The Expression Of Atp10c Mrna During Adipogenesis, A Peretich, Maria Cekanova Ms, Rndr, Phd, S Hurst, Sj Baek, Madhu Dahr

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.

A Switch From Polyandry To Serial Monogamy: Results From A Three Year Tagging Study Of Horseshoe Crabs In Long Island Sound, Jennifer Mattei Ph.D., Paul Goodell, Christine Depierro, Meghann Burke

Biology Faculty Publications

Part of an ongoing population study of the North American Horseshoe Crab, Limulus polyphemus, in Long Island Sound was completed during the 2003 – 2005 spawning seasons at Milford Point, in Milford, CT. Horseshoe crabs range more widely throughout Long Island Sound than expected and exhibit weak site fidelity. Animals originally tagged in Milford were found as far west as Stamford and as far east as Clinton, CT. during the 3 year study. Out of 522 female horseshoe crabs tagged in 2004 only 4 came back to spawn again at Milford Pt. in 2005. The sex ratios of tagged and …

Expression Of G-Protein Inwardly Rectifying Potassium Channels (Girks) In Lung Cancer Cell Lines, Howard Plummer 3rd, Madhu Dhar, Maria Cekanova Ms, Rndr, Phd, Hildegard Schuller

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

BACKGROUND: Previous data from our laboratory has indicated that there is a functional link between the beta-adrenergic receptor signaling pathway and the G-protein inwardly rectifying potassium channel (GIRK1) in human breast cancer cell lines. We wanted to determine if GIRK channels were expressed in lung cancers and if a similar link exists in lung cancer. METHODS: GIRK1-4 expression and levels were determined by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. GIRK protein levels were determined by western blots and cell proliferation was determined by a 5-bromo-2'-deoxyuridine (BrdU) assay. RESULTS: GIRK1 mRNA was expressed in three of six small …

Expression And Localization Of Estrogen Receptor-Alpha Protein In Normal And Abnormal Term Placentae And Stimulation Of Trophoblast Differentiation By Estradiol, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Dc Henley, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Estrogens play an important role in the regulation of placental function, and 17-beta-estradiol (E2) production rises eighty fold during human pregnancy. Although term placenta has been found to specifically bind estrogens, cellular localization of estrogen receptor alpha (ER-alpha) in trophoblast remains unclear. We used western blot analysis and immunohistochemistry with h-151 and ID5 monoclonal antibodies to determine the expression and cellular localization of ER-alpha protein in human placentae and cultured trophoblast cells. Western blot analysis revealed a ~65 kDa ER-alpha band in MCF-7 breast carcinoma cells (positive control). A similar band was detected in five normal term placentae exhibiting strong …

Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single ~52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity …

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.