Laboratory and Basic Science Research Commons^™

In Vitro Plaque Formation On Orthodontic Resin Sealants, Leland W. Pack

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to examine in vitro plaque formation on sealant coated enamel. The plaque growth on different types of sealants placed on enamel was compared to each other and to an unsealed enamel control. The antimicrobial properties of sealants were also studied.

Sealant coated bovine enamel disks were immersed in a S. mutans culture medium for four days. The amount of bacterial plaque formation was quantified by removing the deposit, suspending it in solution with subsequent serial dilution. Aliquots of the dilutions were then spread on blood agar plates and incubated for 48 hours. The number …