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Genetics and Genomics Commons™

Open Access. Powered by Scholars. Published by Universities.®

2017

RNA-seq

Articles 1 - 9 of 9

Full-Text Articles in Genetics and Genomics

Finding Function In The Unknown, Kelly Boyd, Emma Highland, Amanda Misch, Amber Hu, Sushma Reddy, Catherine Putonti Sep 2017

Finding Function In The Unknown, Kelly Boyd, Emma Highland, Amanda Misch, Amber Hu, Sushma Reddy, Catherine Putonti

Catherine Putonti

Through high-throughput RNA sequencing (RNAseq), transcriptomes for a single cell, tissue, or organism(s) can be ascertained at a high resolution. While a number of bioinformatic tools have been developed for transcriptome analyses, significant challenges exist for studies of non-model organisms. Without a reference sequence available, raw reads must first be assembled de novo followed by the tedious task of BLAST searches and data mining for functional information. We have created a pipeline, PyRanger, to automate this process. The pipeline includes functionality to assess a single transcriptome and also facilitate comparative transcriptomic studies.

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Unravelling Organelle Genome Transcription Using Publicly Available Rna-Sequencing Data, Matheus Sanita Lima Aug 2017

Unravelling Organelle Genome Transcription Using Publicly Available Rna-Sequencing Data, Matheus Sanita Lima

Electronic Thesis and Dissertation Repository

The study of organelles helped forge theories of genome evolution because of their unconventional genomes and gene expression regimes. The organelle genomics field (~35 years old) has seen the development of next generation sequencing (NGS) techniques and the consequent skyrocketing of genomic and transcriptomic data. However, these data are being underused in the studies of organelle genome transcription. My thesis investigates how NGS has affected the field of organelle genomics at both the DNA and RNA levels. First, I demonstrate that although organelle genomes are being sequenced as never before, they are un-characterized as they are published mostly as “organelle …

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66th National Poultry Breeder Roundtable Poster_Jibin Zhang.Pdf, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont May 2017

66th National Poultry Breeder Roundtable Poster_Jibin Zhang.Pdf, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont

Jibin Zhang

Fast-growing modern broilers are susceptible to cardiac arrhythmia and heart failure. To understand the developmental regulation related to these problems, cardiac gene expression in Illinois and Ross broilers was investigated at 6 and 21 days post hatch (dph) using RNA-seq (n=5-6 for each group). Because Illinois is a legacy broiler line with isometric heart growth, and Ross is a modern broiler line with a negative allometric heart growth, comparison of gene expression between the two lines and the two time points provides an avenue to understand genetic regulation underlying their different cardiac development.
Sequence data were mapped to the Galgal5 …

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Transcriptome Analysis Reveals Potential Mechanisms Underlying Differential Heart Development In Fast- And Slow-Growing Broilers Under Heat Stress, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont Apr 2017

Transcriptome Analysis Reveals Potential Mechanisms Underlying Differential Heart Development In Fast- And Slow-Growing Broilers Under Heat Stress, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont

Jibin Zhang

Background
Modern fast-growing broilers are susceptible to heart failure under heat stress because their relatively small hearts cannot meet increased need of blood pumping. To improve the cardiac tolerance to heat stress in modern broilers through breeding, we need to find the important genes and pathways that contribute to imbalanced cardiac development and frequent occurrence of heat-related heart dysfunction. Two broiler lines – Ross 708 and Illinois – were included in this study as a fast-growing model and a slow-growing model respectively. Each broiler line was separated to two groups at 21 days posthatch. One group was subjected to heat stress …

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Transcriptome Approach For Identifying Potential Biomarkers For Endocrine Disruption Due To Crude Oil Exposure Using Killifish (Fundulus Heteroclitus)., Frank J. Zadlock Iv Mar 2017

Transcriptome Approach For Identifying Potential Biomarkers For Endocrine Disruption Due To Crude Oil Exposure Using Killifish (Fundulus Heteroclitus)., Frank J. Zadlock Iv

Seton Hall University Dissertations and Theses (ETDs)

The killifish, Fundulus heteroclitus, is a common fish model in aquatic toxicology. However, little is known in this organism about how endocrine disrupting compounds, (EDCs) including polycyclic aromatic hydrocarbons (PAHs), impact the reproductive status from a molecular standpoint. The objective of this project is to apply high throughput sequencing to F. heteroclitus testes to examine the molecular mechanisms that impair reproduction when exposed to crude oil. First, a crude oil exposure experiment was conducted. Following exposure, semi-quantitative PCR was performed to detect changes in gene expression of the following genes known to respond to EDCs: gonadal aromatase (CYP19a), vitellogenin …

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Comparative Transcriptomics Of Elasmobranchs And Teleosts Highlight Important Processes In Adaptive Immunity And Regional Endothermy, Nicholas J. Marra, Vincent P. Richards, Angela Early, Steve M. Bogdanowicz, Paulina D. Pavinski Bitar, Michael J. Stanhope, Mahmood S. Shivji Jan 2017

Comparative Transcriptomics Of Elasmobranchs And Teleosts Highlight Important Processes In Adaptive Immunity And Regional Endothermy, Nicholas J. Marra, Vincent P. Richards, Angela Early, Steve M. Bogdanowicz, Paulina D. Pavinski Bitar, Michael J. Stanhope, Mahmood S. Shivji

Biology Faculty Articles

Background: Comparative genomic and/or transcriptomic analyses involving elasmobranchs remain limited, with genome level comparisons of the elasmobranch immune system to that of higher vertebrates, non-existent. This paper reports a comparative RNA-seq analysis of heart tissue from seven species, including four elasmobranchs and three teleosts, focusing on immunity, but concomitantly seeking to identify genetic similarities shared by the two lamnid sharks and the single billfish in our study, which could be linked to convergent evolution of regional endothermy.

Results: Across seven species, we identified an average of 10,877 Swiss-Prot annotated genes from an average of 32,474 open reading frames within each …

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Genomics Rna-Seq Analysis Part 1-Cyverse De, Fastqc, Trimmomatic (Updated 11/17), Ray A. Enke, Melika Rahmani-Mofrad Dec 2016

Genomics Rna-Seq Analysis Part 1-Cyverse De, Fastqc, Trimmomatic (Updated 11/17), Ray A. Enke, Melika Rahmani-Mofrad

Ray Enke Ph.D.

This in class exercise is designed to teach students about the basic features of CyVerse Discovery Environment (DE) as well as how to run FastQC and Trimmomatic steps of a general eukaryotic RNA-seq analysis pipeline using CyVerse DE apps.

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Genomics Rna-Seq Analysis Part 2_ Kallisto Indexing And Quantification (Updated 11/17), Ray A. Enke, Melika Rahmani-Mofrad Dec 2016

Genomics Rna-Seq Analysis Part 2_ Kallisto Indexing And Quantification (Updated 11/17), Ray A. Enke, Melika Rahmani-Mofrad

Ray Enke Ph.D.

This in class exercise is a hands on activity designed to teach students about how to run Kallisto indexing quantification using CyVerse DE apps as part of a eukaryotic RNA-seq analysis pipeline.

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Genomics Rna-Seq Analysis Part 3-Sleuth Data Visualization (Updated 11/17), Ray A. Enke, Scott Schumacker Dec 2016

Genomics Rna-Seq Analysis Part 3-Sleuth Data Visualization (Updated 11/17), Ray A. Enke, Scott Schumacker

Ray Enke Ph.D.

This in class exercise is a hands on activity designed to teach students about how to run Sleuth statistical modeling and RStudio data visualization package using Kallisto pseudoalignment output files as part of a eukaryotic RNA-seq analysis pipeline.

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