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Articles 1 - 8 of 8
Full-Text Articles in Genetics and Genomics
Electrically Mediated Delivery Of Vector Plasmid Dna Elicits An Antitumor Effect, L. Heller, D. Coppola
Electrically Mediated Delivery Of Vector Plasmid Dna Elicits An Antitumor Effect, L. Heller, D. Coppola
Bioelectrics Publications
In vivo electroporation is an efficient means of increasing plasmid DNA delivery to normal tissues, such as skin and muscle, as well as directly to tumors. In the experiments described here, plasmid DNA was delivered by in vivo electroporation to B16 mouse melanomas using two very different pulsing protocols. Reporter expression increased 21- or 42-fold, respectively with electroporation over injection alone. The growth of experimental melanomas with an approximate diameter of 4 mm on the day of treatment was monitored after electroporation delivery of reporter plasmid DNA. Remarkably, short-term complete regressions using one of these pulsing protocols occurred in up …
Jun Dimerization Protein 2 Functions As A Progesterone Receptor N-Terminal Domain Coactivator, James S. Adelman, Suzanne E. Wardell, Viroj Boonyaratanakornkit, Ami Aronheim
Jun Dimerization Protein 2 Functions As A Progesterone Receptor N-Terminal Domain Coactivator, James S. Adelman, Suzanne E. Wardell, Viroj Boonyaratanakornkit, Ami Aronheim
James S. Adelman
The progesterone receptor (PR) contains two transcription activation function (AF) domains, constitutive AF-1 in the N terminus and AF-2 in the C terminus. AF-2 activity is mediated by a hormone-dependent interaction with a family of steroid receptor coactivators (SRCs). SRC-1 can also stimulate AF-1 activity through a secondary domain that interacts simultaneously with the primary AF-2 interaction site. Other protein interactions and mechanisms that mediate AF-1 activity are not well defined. By interaction cloning, we identified an AP-1 family member, Jun dimerization protein 2 (JDP-2), as a novel PR-interacting protein. JDP-2 was first defined as a c-Jun interacting protein that …
Mechanism Of Abietadiene Synthase Catalysis: Stereochemistry And Stabilization Of The Cryptic Pimarenyl Carbocation Intermediates, Reuben J. Peters, Matthew M. Ravn, Robert M. Coates, Rodney Croteau
Mechanism Of Abietadiene Synthase Catalysis: Stereochemistry And Stabilization Of The Cryptic Pimarenyl Carbocation Intermediates, Reuben J. Peters, Matthew M. Ravn, Robert M. Coates, Rodney Croteau
Reuben J. Peters
Abietadiene synthase (AS) catalyzes the complex cyclization-rearrangement of (E,E,E)-geranylgeranyl diphosphate (8, GGPP) to a mixture of abietadiene (1a), double bond isomers 2a-4a and pimaradienes 5a-7a as a key step in the biosynthesis of the abietane resin acid constituents (1b-4b) of conifer oleoresin. The reaction proceeds at two active sites by way of the intermediate, copalyl diphosphate (9). In the second site, a putative tricyclic pimaradiene or pimarenyl(+) carbocation intermediate of undefined C13 stereochemistry and annular double bond position is formed. Three 8-oxy-17-nor analogues of 9 (17 and 19a,b) and three isomeric 15,16-bisnorpimarenyl-N-methylamines (26a-c) were synthesized and evaluated as alternative substrates …
Production Of A Monoclonal Antibody Against Benzo[Α]Pyrene Diol Epoxide Dna Adducts, Brian Peden Austin
Production Of A Monoclonal Antibody Against Benzo[Α]Pyrene Diol Epoxide Dna Adducts, Brian Peden Austin
Chemistry & Biochemistry Theses & Dissertations
Benzo[α]pyrene is a ubiquitous pollutant produced from the incomplete combustion of organic material such as fossil fuels. It is found in the workplace, urban air, drinking water, and the food supply. Recently, it has been proposed that benzo[α]pyrene may be the causative agent in the formation of lung adenocarcinomas among some Taiwanese women exposed to cooking oil fumes without adequate ventilation. In this study, calf thymus DNA was modified in vitro with benzo[α]pyrene-diol epoxide (BPDE) to a level consistent with that found in biological samples. This DNA of low modification was used as an immunogen in the production of a …
Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder
Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder
Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology
No abstract provided.
Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder
Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder
Maria Cekanova MS, RNDr, PhD
No abstract provided.
Genetic Stock Structure Of The Sailfish, Istiophorus Platypterus, Based On Nuclear And Mitochondrial Dna, Jan Renee Mcdowell
Genetic Stock Structure Of The Sailfish, Istiophorus Platypterus, Based On Nuclear And Mitochondrial Dna, Jan Renee Mcdowell
Dissertations, Theses, and Masters Projects
The results of life history studies involving the sailfish, Istiophorus platypterus, from the Atlantic, Pacific, and Indian oceans were reviewed. Results of studies agree on several aspects of sailfish biology; sailfish exhibit opportunistic feeding behavior, follow the 28??C isotherm, spawn in summer mature at approximately age 3, and are the least migratory of the billfishes. However, results are in conflict concerning sex ratio and whether sailfish are single or multiple spawners. Although many researchers have reported that sailfish are sexually dimorphic, their fragile otoliths make them difficult to age, aging studies have not been validated, and sexual dimorphism has not …
Molecular And Morphological Evolution Of The Amphipod Radiation Of Lake Baikal, Kenneth S. Macdonald Iii
Molecular And Morphological Evolution Of The Amphipod Radiation Of Lake Baikal, Kenneth S. Macdonald Iii
Dissertations, Theses, and Masters Projects
The amphipods of Lake Baikal, Russia, are a taxonomically and morphologically diverse group, and have been cited as a classic example of adaptive radiation. An unfortunate consequence of this diversity has been an instability of Baikal amphipod classification, with frequent revisions spurred by differing opinions about the phylogenetic utility of different characters. Classifications have never been based on a rigorous phylogenetic analysis of morphological data and only recently has the phylogeny of Lake Baikal's amphipods been examined using molecular tools. Unfortunately, most of these studies have led to a limited expansion of our understanding of Baikal amphipod systematics. Phylogenetic relationships …