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Full-Text Articles in Genetics and Genomics
Regulation Of D-Xylose Metabolism In Caulobacter Crescentus By A Laci-Type Repressor, Craig Stephens, Beat Christen, Kelly Watanabe, Thomas Fuchs, Urs Jenal
Regulation Of D-Xylose Metabolism In Caulobacter Crescentus By A Laci-Type Repressor, Craig Stephens, Beat Christen, Kelly Watanabe, Thomas Fuchs, Urs Jenal
Biology
In the oligotrophic freshwater bacterium Caulobacter crescentus, D-xylose induces expression of over 50 genes,including the xyl operon, which encodes key enzymes for xylose metabolism. The promoter (PxylX) controllingexpression of the xyl operon is widely used as a tool for inducible heterologous gene expression in C. crescentus. We show here that PxylX and at least one other promoter in the xylose regulon (PxylE) are controlled by theCC3065 (xylR) gene product, a LacI-type repressor. Electrophoretic gel mobility shift assays showed that operator binding by XylR is greatly reduced in the presence of D-xylose. The data support the hypothesis that there is a …
Genetic Analysis Of A Novel Pathway For D-Xylose Metabolism In Caulobacter Crescentus, Craig Stephens, Beat Christen, Thomas Fuchs, Vidyodhaya Sundaram, Kelly Watanabe, Urs Jenal
Genetic Analysis Of A Novel Pathway For D-Xylose Metabolism In Caulobacter Crescentus, Craig Stephens, Beat Christen, Thomas Fuchs, Vidyodhaya Sundaram, Kelly Watanabe, Urs Jenal
Biology
Genetic data suggest that the oligotrophic freshwater bacterium Caulobacter crescentus metabolizes D-xylose through a pathway yielding -ketoglutarate, comparable to the recently described L-arabinose degradation pathway of Azospirillum brasilense. Enzymes of the C. crescentus pathway, including an NAD -dependent xylose dehydrogenase, are encoded in the xylose-inducible xylXABCD operon (CC0823-CC0819).