Genetics and Genomics Commons^™

Methylation-Specific Differentiation Of Vaginal Epithelial Cells For Forensic Tissue Typing By Bisulfite Conversion And Pyrosequencing, Elise Pood

Master's Theses

The identification of bodily fluids and tissues is often applied to criminal investigations to clarify events that may or may not have taken place. Current forensic techniques can identify blood, saliva, seminal fluid, and spermatozoa, but there is a clear absence of reliable testing to identify vaginal epithelial tissue. Though there are serological tests available for this purpose, tissue-specific methylation markers have recently been investigated as a candidate for the identification of blood, saliva, and spermatozoa.

In this study, tissue-specific methylation markers were analyzed to identify a set of markers for the differentiation of vaginal fluid from blood, saliva, and …

Fate Of Ingested Rna In The Two-Spotted Spider Mite, Obrie D. Scarbrough

Honors Theses

RNA interference, or RNAi, is a gene regulation mechanism that uses small noncoding RNAs (sRNAs) to silence the expression of certain genes. The application of RNAi has been extended to insect pest control. The two-spotted spider mite Tetranychus urticae is a persistent agricultural pest that tends to develop pesticide resistance at an alarming rate, making it a perfect candidate for RNAi technology development. It was hypothesized that unique sRNAs could be isolated from RNA soaked spider mites, and new synthetic RNAs could be synthesized to elicit greater knockdown than was achieved in previous studies. To perform this research, a small …

Tadkb:Family Classification And A Knowledge Base Of Topologically Associating Domains, Tong Liu, Jacob Porter, Chenguang Zhao, Hao Zhu, Nan Wang, Zheng Sun, Yin-Yuan Mo, Zheng Wang

Faculty Publications

Background: Topologically associating domains (TADs) are considered the structural and functional units of the genome. However, there is a lack of an integrated resource for TADs in the literature where researchers can obtain family classifications and detailed information about TADs.

Results: We built an online knowledge base TADKB integrating knowledge for TADs in eleven cell types of human and mouse. For each TAD, TADKB provides the predicted three-dimensional (3D) structures of chromosomes and TADs, and detailed annotations about the protein-coding genes and long non-coding RNAs (lncRNAs) existent in each TAD. Besides the 3D chromosomal structures inferred by population …